IPSWICH, Mass., Aug. 8, 2012 /PRNewswire/ -- New England Biolabs (NEB) has released a novel DNA polymerase, Q5™ High-Fidelity DNA Polymerase, that provides the highest fidelity amplification currently available, while also offering robust amplification across the broadest range of amplicons, including those that are GC- and AT-rich.
High fidelity amplification is essential for experiments whose outcome depends upon the correct DNA sequence, such as sequencing, SNP detection, protein expression and gene function studies. However, results from such amplifications can be compromised by a DNA polymerase incapable of evenly amplifying diverse types of sequences. For example, high-fidelity PCR of GC-rich regions and other challenging amplicons can lead to lower yields and even non-specific amplification. In addition, any bias in library amplification, associated with next generation sequencing, can lead to challenges in sequence assembly and even "missing" sequence, a problem of growing concern in the sequencing community.
NEB's novel polymerase is fused to the processivity-enhancing Sso7d DNA binding domain, which improves fidelity (more than 50-fold higher than Taq), speed, and performance reliability (amplifying targets up to 20 kb long with extension times as low as 10 seconds per kb). The Q5 buffer system has also been extensively optimized to provide robust performance across both high-GC and high-AT regions, as well as other challenging amplicons.
"High-fidelity polymerases have tended to be more susceptible to difficulties in the PCR amplification of GC-rich or other challenging amplicons. Q5 polymerase has dramatical
|SOURCE New England Biolabs|
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