Navigation Links
Nano detector for deadly anthrax
Date:8/14/2011

An automatic and portable detector that takes just fifteen minutes to analyze a sample suspected of contamination with anthrax is being developed by US researchers. The technology amplifies any anthrax DNA present in the sample and can reveal the presence of just 40 microscopic cells of the deadly bacteria Bacillus anthracis.

B. anthracis, commonly known as anthrax, is a potentially lethal microbe that might be used intentionally to infect victims through contamination of food and water supplies, aerosolized particles, or even dried powders, such as those used in bioterrorist attacks in the USA. Detection is crucial to preventing widespread fatalities in the event of an anthrax attack. However, the complexity of the microbe's biology have so far made it difficult to build a portable system that can be employed quickly in the field. That said, there are several systems available that use PCR to amplify a particular component of the genetic material present in anthrax and then to flag this amplified signal. These systems are fast and sensitive but do not integrate sample preparation and so are not as convenient as a single detector unit would be.

Writing in the International Journal of Biomedical Nanoscience and Nanotechnology this month, Nathaniel Cady of the College of Nanoscale Science and Engineering (CNSE) of the University at Albany and colleagues there and at Cornell University, New York, explain how they have constructed nanofabricated fluidic cartridges that can be used to carry out detection of anthrax. The device is a so-called "lab-on-a-chips" device, or more properly a 3D microfluidic network that contains nanofabricated pillar structures.

The device has fluidic inputs for adding sample and reagents, removing waste, for carrying out DNA purification, and critically an integrated chamber for amplifying only the target DNA in the sample using the polymerase chain reaction (PCR) system. The chip also contains a wave guide for the fluorescence-based identification of the amplified DNA and thus the target microbe. Importantly, the system works without manual intervention other than loading a droplet of sample into the detector.

"The average time required for DNA purification during these experiments was approximately 15 min, and when combined with real-time PCR analysis, this resulted in an average time to detection of 60 min," the team says. The system can detect as few as 40 B. anthracis cells. "Due to its small size and low power requirements, this system can be further developed as a truly portable, hand-held device," the researchers conclude.


'/>"/>

Contact: Nathaniel C. Cady
ncady@uamail.albany.edu
Inderscience Publishers
Source:Eurekalert

Related biology technology :

1. Livermore researchers develop battery-less chemical detector
2. Ultra fast photodetectors out of carbon nanotubes
3. Rice physicists discover ultrasensitive microwave detector
4. Extreme darkness: Carbon nanotube forest covers NISTs ultra-dark detector
5. Looking to leap forward on laser and photodetector technologies
6. Nikon Instruments Introduces Exclusive VAAS Detector Imaging Method for Confocal Microscopy
7. Making blood-sucking deadly for mosquitoes
8. Computational Microbiologist Uses His Expertise with Deadly Viruses to Devise a Superior System for Delivery of Safe, Free Software Downloads
9. Detection of potentially deadly atrial fibrillation dramatically improved by new algorithm
10. Germgards Ultra-Fast Hand Hygiene Solution Protects Against Deadly MRSA, C difficile, and Influenza Virus
11. PharmAthene Achieves Anthrax Vaccine Program Milestone and Successfully Completes Technology Transfer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/9/2016)... ... 09, 2016 , ... DuPont Industrial Biosciences (DuPont) and Archer ... Year Award from Platts Global Energy for their platform technology to produce ... at the 18th Platts Global Energy Awards, held in New York on Thursday, Dec. ...
(Date:12/9/2016)... 2016 The research report by ... in the  Global Label-Free Array Systems Market  accounted for ... 2015. Players such as Biacore, Agilent Technologies, ForteBio, Molecular ... the global market due to their unmatched product portfolio ... upgrades and timely product launches are expected to be ...
(Date:12/9/2016)... 2016 China Cord Blood Corporation (NYSE: ... leading provider of cord blood collection, laboratory testing, hematopoietic ... the results of its 2016 Annual General Meeting, which was ... China . At the Annual ... of KPMG Huazhen LLP as the independent auditors of the ...
(Date:12/9/2016)... According to a new market research report ... Reagent Equipment), Application (Research, PCR, Gene, DNA, NGS, Diagnostic, RNAI), End ... the global market is expected to reach USD 2.20 Billion by ... 10.6% during the forecast period. Continue ... ...
Breaking Biology Technology:
(Date:12/8/2016)... -- Singulex, Inc., the leader in Next Generation Immunodiagnostics powered ... and supply agreement with Thermo Fisher Scientific, the world ... to Thermo Scientific BRAHMS PCT (Procalcitonin), a biomarker which, ... diagnose systemic bacterial infection and sepsis and in ... assessing the risk of critically ill patients for progression ...
(Date:12/7/2016)... , Dec. 7, 2016   Veridium ... announced the appointment of new CEO James ... executive with decades of experience, has served in ... Cisco, where he specialized in expanding a pipeline ... technology portfolios. He most recently served as managing ...
(Date:12/5/2016)... 5, 2016  The Office of Justice Programs, ... CT Scans Enhance or Replace Medico Legal Autopsies?" ... supporting or replacing forensic autopsies with postmortem X-ray ... In response to recommendations made by The ... CT scans as a potential component of medicolegal ...
Breaking Biology News(10 mins):