Navigation Links
NSG's Releases New Toluene in Hexane Reference Standard for Resolution and Spectral Bandwidth Verification
Date:6/25/2013

Farmingdale, NY (PRWEB) June 25, 2013

The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve absorption lines separated by small differences in wavelength. If a substance that requires measurement has an absorption line at 257.4nm and there is an interfering line at 260.0 nm, one needs to establish whether the spectrophotometer can resolve the two lines separately or if it "mixes" the two absorption lines into a single line. This brings about erroneous results. Toluene in Hexane is used as a reference for the calculation of SBW. The ratio of the absorption of the solution when read at a maxima (around 268.7nm) and at a minima (around 267.0nm) relates directly to the SBW of the instrument being assessed. Regular use of this technique will assure that the resolution of your instrument is within the required range for Good Laboratory Practice.

Assessing the photometric and wavelength performance of a UV/VIS spectrophotometer is a necessary part of keeping a laboratory in accordance with GLP standards which makes NSG Precision Cells' Toluene in Hexane a necessity. Understanding and accounting for how expanded uncertainties affect these measurements is a critical component of the calibration process.

NSG's Toluene in Hexane reference standard ensures accurate wavelength and absorbance results by verifying the spectrophotometer's resolution. Accurate absorbance and wavelength measurements can only be achieved if due consideration is given to the resolution of the monochromator in use. In modern instruments with grating monochromators, the resolution relates directly to the slit width chosen. The smaller the slit and associated spectral bandwidth, the greater the resolution, but the corresponding reduction in energy means the signal-to-noise ratio falls. When measuring an absorbance band in a high-resolution instrument, it is recommended that the spectral bandwidth (SBW) should not exceed 10% of the natural bandwidth (NBW) of the band. Simple checks of the resolution and bandwidth of your spectrophotometer will ensure that the best possible results are obtained for your analyses. A 0.02% Toluene in Hexane Solution is the European Pharmacopoeia's preferred method for verifying resolution of a spectrophotometer.

Toluene in Hexane is Heat-sealed in a quartz cuvette and traceable to the NIST. Resolution is measured by calculating the ratio of the peak maxima of toluene at 269nm to its peak minima at 266nm. Resulting ratio provides a measure of instrument resolution. To determine spectral bandwidth, compare ratio obtained with table provided with the standard.

The EP recommends comparing the ratio of the peak maxima at 269nm and the minimum at 266nm to determine the spectral resolution of your spectrophotometer. Determine the resolution by running the toluene in hexane against the hexane blank between 265-270nm and calculate the peak minima and maxima. Calculate the ratios of the peak to the trough and compare these results with the reference values. Please note that this is a liquid calibration filter and does not come with the same Lifetime Warranty as NSG's Solid State Standards. The NSG Toluene in Hexane filter must be recalibrated every year according to NIST guidelines.

About NSG Precision Cells

NSG Precision Cells, Inc. was founded in 1958 and has been growing ever since. NSG Precision Cells, Inc. is a well established, highly regarded quartz manufacturer and we specialize in the manufacturing of quartz flowcells, quartz flow channel cuvettes, quartz microchips, quartz cuvettes, and other quartz optical products.

Read the full story at http://www.prweb.com/releases/2013/6/prweb10835537.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. HealthCare Institute of New Jersey Releases 2011 Biopharmaceutical and Medical Technology Economic Impact Data
2. HCCS Releases ICD-10 Countdown Clock With Integrated CMS Twitter Tweets
3. eCareSoft Releases eCS Cirrus™
4. AtheroNova Releases 2011 Financial Results
5. Atlanta LASIK Surgeon Dr. Ashraf Releases YouTube Videos Featuring Patient Testimonials and Discussion of Common Eye Treatments
6. ILVO-T&V Belgium Releases Validation Report of the Charm Sciences Beta-lactam and Tetracycline 2-minute Test for Raw Commingled Milk
7. ISPE Releases New Guidance for Designing and Constructing Packaging, Labeling and Warehousing Facilities
8. Happtique Releases Draft App Certification Program Standards For Public Comment
9. ClearCanvas Releases New Workstation Products Designed to Assist With Image Sharing
10. OneMedPlace Releases Update to Research Report on Amarantus Biosciences Focused on New Positive Ischemic Heart Disease Efficacy Data for MANF in Animals
11. Weight Loss: Natural Way To Lose More Weight Than With Diet & Exercise Alone - Consumer Reporter Releases A New Video
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/23/2017)... NEW YORK , Feb. 23, ... , a leading digital health company, and ... of telemedicine and remote patient monitoring, announce they ... reimbursements.  DN Telehealth maximizes collaboration ... real-time, extending consultations beyond a physical clinical setting ...
(Date:2/23/2017)... JOSE, Calif. , Feb. 23, 2017 /PRNewswire/ ... exclusive license for two key immunotherapy technologies from ... first technology provides a method to monitor a ... such as PD-L1 and CTLA-4.  The second license ... if a patient is likely to have an ...
(Date:2/23/2017)... ... February 23, 2017 , ... Brain Sentinel, ... begin marketing the SPEAC® System, the Brain Sentinel® Seizure Monitoring and Alerting System. ... healthcare facilities during periods of rest. A lightweight, non-invasive monitor is placed on ...
(Date:2/23/2017)... ... February 23, 2017 , ... The Greater Gift ... new partnership with Compass Research . GGI's mission is to advance global health ... a child in need in honor of each clinical trial volunteer. The vision of ...
Breaking Biology Technology:
(Date:2/8/2017)... , Feb. 7, 2017 The biometrics ... by the confluence of organizations, desires to better ... knowledge-based systems (password and challenge questions), biometrics is ... government systems. The market is driven by use ... between consumer and enterprise uses cases, with consumer-facing ...
(Date:2/7/2017)... BEACH, New York , February 7, 2017 /PRNewswire/ ... known as ID Global Solutions Corporation [OTC: IDGS], ("Ipsidy" ... identity management and electronic transaction processing services, is pleased ... reorganization of the Company. Effective January 31, ... of the Board of Directors, CEO and President.  An ...
(Date:2/2/2017)... Feb. 2, 2017   TapImmune, Inc. ... company specializing in the development of innovative peptide ... of cancer and metastatic disease, announced today it ... manufacturing of a second clinical lot of TPIV ... receptor alpha. The manufactured vaccine product will be ...
Breaking Biology News(10 mins):