Navigation Links
Molecular 'eat now' signal makes cells devour dying neighbors

A team of researchers has devised a Pac-Man-style power pellet that gets normally mild-mannered cells to gobble up their undesirable neighbors. The development may point the way to therapies that enlist patients' own cells to better fend off infection and even cancer, the researchers say.

A description of the work will be published July 15 in the journal Science Signaling.

"Our goal is to build artificial cells programmed to eat up dangerous junk in the body, which could be anything from bacteria to the amyloid-beta plaques that cause Alzheimer's to the body's own rogue cancer cells," says Takanari Inoue, Ph.D., an associate professor of cell biology in the Johns Hopkins University School of Medicine's Institute for Basic Biomedical Sciences, who led the study. "By figuring out how to get normally inert cells to recognize and engulf dying cells, we've taken an important step in that direction."

Identifying and devouring dying cells and other "junk" is usually the job of white blood cells called macrophages and neutrophils, which also go after bacteria and other invaders in a process called phagocytosis. For the new experiments, Inoue teamed up with researchers at the University of Tokyo to strip down phagocytosis, figuring out the minimum tools one cell needs to eat another one.

They started not with macrophages, but with a type of laboratory-grown human cells known as HeLa, which normally can't perform phagocytosis. Their first task was to induce the HeLa cells to attach to nearby dying cells by getting the right "receptors" to the HeLa cells' surface. The researchers knew that part of a receptor protein called MFG-E8 would recognize and stick to a distress signal on the surface of dying cells, and coaxing the HeLa cells to make the protein fragment was straightforward. To get the fragment, termed C2, onto the outside of the cells, the team found a way to stick it to another protein that was bound for the cell's surface, thus taking advantage of the cell's own transportation system. "We put C2 on the same bus as the membrane protein," Inoue says.

As a result, up to six dying cells stuck to each HeLa cell. The bad news was that though they were cozy, the HeLa cells weren't actually eating the dying cells.

Fortunately, Inoue says, the team already had an idea about what to try next: Other research had shown that activating a gene called Rac would cause a cell to engulf beads stuck to its surface. Sure enough, HeLa cells with both surface C2 and activated Rac swallowed dying cells readily, the team found.

"We've shown it's possible to endow ordinary cells with the power to do something unique: take on the role of a specialized macrophage," Inoue says.

Inoue cautions that the investigators don't believe the engulfed cells are being broken down. Getting the HeLa cells to finish the phagocytosis process will be one of the group's next steps.


Contact: Shawna Williams
Johns Hopkins Medicine

Related biology technology :

1. Rice nanophotonics experts create powerful molecular sensor
2. BerGenBio Presents Data at AACR-ISLAC Conference on the Molecular Origins of Lung Cancer
3. Life Technologies to Provide Ion Torrent Sequencing Platform to Quest Diagnostics for Development of New Molecular Tests
4. York U molecular communication researchers send worlds first text message using vodka
5. Caris Life Sciences Receives Patent for Molecular Profiling Service in The Peoples Republic of China
6. Molecular Diagnostics Markets
7. Cynvenio and CollabRx Announce Partnership for Cancer Molecular Diagnostics
8. Webinar: Blood-Based Proteomic Classifier for the Molecular Characterization of Pulmonary Nodules, a Case Study in the Discovery and Validation of a Molecular Diagnostic
9. Molecular Diagnostics - Global Strategic Business Report
10. Biomedical Research Foundation (BRF) Recruits Two Eminent Scientists --Pradeep Garg, Ph.D., And Sudha Garg, Ph.D. -- To Run Newly-Created Louisiana Center For Molecular Imaging And Therapy
11. 2014 Analysis of the European Molecular Diagnostics Market: Segment Forecasts and Supplier Shares
Post Your Comments:
Related Image:
Molecular 'eat now' signal makes cells devour dying neighbors
(Date:6/27/2016)... June 27, 2016 /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" ... by its major shareholders, Clean Technology Fund I, LP ... States based venture capital funds which together ... (on a fully diluted, as converted basis), that they ... their entire equity holdings in Biorem to TUS Holdings ...
(Date:6/27/2016)... ... June 27, 2016 , ... Newly created 4Sight Medical ... to the healthcare market. The company's primary focus is on new product introductions, ... strategies that are necessary to help companies efficiently bring their products to market. ...
(Date:6/24/2016)...  Regular discussions on a range of subjects including policies, ... entities said Poloz. Speaking at a lecture to ... he pointed to the country,s inflation target, which is set ... "In certain areas there needs ... economic goals, why not sit down and address strategy together?" ...
(Date:6/23/2016)... ... 23, 2016 , ... Mosio, a leader in clinical research ... Recruitment and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the ... tools, and strategies for clinical researchers. , “The landscape of how patients receive ...
Breaking Biology Technology:
(Date:6/1/2016)... Favorable Government Initiatives Coupled With ... Identification to Boost Global Biometrics System Market Through 2021  ... report, " Global Biometrics Market By Type, By ... 2011 - 2021", the global biometrics market is projected ... of growing security concerns across various end use sectors ...
(Date:5/9/2016)... , UAE, May 9, 2016 ... it comes to expanding freedom for high net worth ... Even in today,s globally connected world, there is still ... system could ever duplicate sealing your deal with a ... second passports by taking advantage of citizenship via investment ...
(Date:4/26/2016)... 2016 Research and Markets has ... Market 2016-2020"  report to their offering.  , ,     ... The analysts forecast the global multimodal biometrics market ... the period 2016-2020.  Multimodal biometrics is ... as the healthcare, BFSI, transportation, automotive, and government ...
Breaking Biology News(10 mins):