Navigation Links
Micropharma completes development of first microbiome clinical lab test using digital PCR technology

MONTREAL, Oct. 23, 2013 /PRNewswire-iReach/ -- Micropharma Limited, a pioneer in the development of microbiome based technologies announced today the completed development of a new lab test to quantify bile salt hydrolase (bsh) gene abundance in gut-derived samples. bsh is a bacterial gene that produces an enzyme that is active in the human GI tract and modifies the bile acid population to provide a number of health benefits to the host organism. Scientific reports have recently shown that individuals with inflammatory and metabolic ailments including inflammatory bowel disease, irritable bowel syndrome, hypercholesterolemia and type II diabetes present low quantities of bsh gene in their gastrointestinal microbiome. Microbiome SenseIT bsh, the lab test, will serve to provide health care practitioners and patient with additional information regarding the abundance of the gene. SenseIT bsh is the first of its kind to use Bio-Rad droplet digital PCR technology which allows for absolute quantification of gene copies with accuracies of 95%.

Using bioinformatics tools, researchers at Micropharma identified the genetic sequences of the bsh genes present in the gut to develop Microbiome SenseIT bsh. The test was validated using metagenomic sequencing and qPCR technologies. Currently, the gene quantification is done by sequencing DNA extracted from gut-derived samples followed by a computer-intense bioinformatics analysis which is time consuming and costly. Microbiome SenseIT bsh can produce results within a few hours at a fraction of the cost of sequencing. The test consists of producing approximately 20000 nanoliter-volume droplets, which are filled with a diluted DNA sample. After droplet generation, PCR amplification follows using gene specific primers and fluorescently-labeled probes, so that the droplets containing the bsh gene become fluorescent. Finally, bsh positive and negative droplets are numbered in a droplet counter to produce the final result. Dr. Ganopolsky was quoted as saying "The technique is so sensitive that can detect only one copy of the gene without false positives". In addition, the accuracy of droplet digital PCR is up to 10 times higher than the traditional qPCR, which is necessary in this type of analysis. Micropharma aims to expand the development of the SenseIT platform which represents the first approach to personalized medicine tests based on microbiome analysis.

About Micropharma Limited

Micropharma is a Canadian biotechnology company focused on human health through the microbiome. The company applies a unique approach, ProSelect, to the discovery and commercialization of novel products based on the human microbiome for the diagnosis and treatment of metabolic diseases.  Micropharma works on disease specific products with clear microbiome related mechanisms, and links to biomarkers that are associated with improving human health.  Micropharma is developing diagnostics and oral biologic therapies in the area of metabolic disease including hypercholesterolemia, metabolic syndrome, obesity, and diabetes.  For more info go to

Media Contact: Mitchell Jones, Micropharma Limited, 514-927-5333,

News distributed by PR Newswire iReach:

SOURCE Micropharma Limited
Copyright©2012 PR Newswire.
All rights reserved

Related biology technology :

1. Micropharma Convenes Expert Roundtable on New Role for Probiotics in Cardiovascular Disease in time for National Cholesterol Education Month
2. Micropharma announces timeline to commercial launch of worlds first ingestible gastrointestinal sampling device
3. Micropharma Limited announces new agreement to commercialize novel natural health products
4. Biocartis Completes EUR 71 Million (USD 100 Million) Series C Fund Raising
5. InnoPharma Completes $15 Million Series A Financing
6. Aratana Therapeutics Completes $15 Million Series B Financing
7. CeNeRx BioPharma Completes $4.85 Million Financing
8. PDL BioPharma Completes Regular Quarterly Dividend Payment
9. MiMedx Group Completes $5,000,000 Private Placement
10. goBalto Completes a Successful FDA 21 CFR Part 11 Compliance Audit
11. Auxogyn Completes $20 Million In Series A Funding
Post Your Comments:
(Date:6/23/2016)... -- The Prostate Cancer Foundation (PCF) is pleased to announce 24 new ... prostate cancer. Members of the Class of 2016 were selected from a pool ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... 23, 2016   EpiBiome , a precision microbiome ... in debt financing from Silicon Valley Bank (SVB). The ... to advance its drug development efforts, as well as ... "SVB has been an incredible strategic partner to ... traditional bank would provide," said Dr. Aeron Tynes ...
(Date:6/23/2016)... OTTAWA, ON (PRWEB) , ... June 23, 2016 , ... ... former DNA Technical Leader at the Arkansas State Crime Laboratory, has joined STACS DNA ... joining the STACS DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. ...
(Date:6/23/2016)... June 23, 2016 Apellis Pharmaceuticals, Inc. ... clinical trials of its complement C3 inhibitor, APL-2. ... multiple ascending dose studies designed to assess the ... subcutaneous injection in healthy adult volunteers. ... as a single dose (ranging from 45 to ...
Breaking Biology Technology:
(Date:6/2/2016)... The Weather Company , an IBM Business (NYSE: IBM ... which consumers will be able to interact with IBM Watson ... or text and receive relevant information about the product or ... long sought an advertising solution that can create a one-to-one ... valuable; and can scale across millions of interactions and touchpoints. ...
(Date:5/20/2016)...  VoiceIt is excited to announce its new ... By working together, VoiceIt and VoicePass will offer ... take slightly different approaches to voice biometrics, collaboration ... usability. Both ... "This marketing and technology partnership allows ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
Breaking Biology News(10 mins):