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Microbes reprogrammed to ooze oil for renewable biofuel
Date:3/29/2010

iny biofuel production facilities.

Liu realized that if cyanobacteria could be cajoled into overproducing fatty acids, their accumulation within the cells would eventually cause these fatty acids to leak out through the cell membrane, through the process of diffusion. To accomplish this, Liu introduced a specific enzyme, known as thioesterase, into cyanobacteria.

The enzyme is able to uncouple fatty acids from complex carrier proteins, freeing them within the cell where they accumulate, until the cell secretes them. "I use genes that can steal fatty acids from the lipid synthesis pathway," Liu explains noting that thioesterase acts to efficiently clip the bonds associating the fatty acids with more complex molecules. This use of modified thioesterases to cause secretion of fatty acids was first described for Escherichia coli by John Cronan of the University of Illinois more than a decade ago.

A second series of modifications enhances the secretion process, by genetically deleting or modifying two key layers of the cellular envelopeknown as the S and peptidoglycan layersallowing fatty acids to more easily escape outside the cell, where their low water solubility causes them to precipitate out of solution, forming a whitish residue on the surface. Study results show a 3-fold increase in fatty acid yield, after genetic modification of the two membrane layers.

To improve the fatty acid production even further, the group added genes to cause overproduction of fatty acid precursors and removed some cellular pathways that were non-essential to the survival of cyanobacteria. Such modifications ensure that the microbe's resources are devoted to basic survival and lipid production.

Liu emphasizes that the current research has moved along at a lightening clip, with only about 6 months passing from the initial work, through production of the first strainsa fact he attributes to the formidable expertise in the area of micr
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Contact: Joe Caspermeyer
joseph.caspermeyer@asu.edu
480-258-8972
Arizona State University
Source:Eurekalert  

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