Guelph, Ontario--Just because you don't swallow the worm at the bottom of a bottle of mescal doesn't mean you have avoided the essential worminess of the potent Mexican liquor, according to scientists from the Biodiversity Institute of Ontario (BIO) at the University of Guelph.
They have discovered that the liquid itself contains the DNA of the agave butterfly caterpillar the famously tasty mescal "worm".
As part of a study to test their theory that DNA from a preserved specimen can leak into its preservative liquid, the BIO team tested a sample of liquid from a bottle of Monte Alban brand mescal. The liquor was found to contain DNA, which they amplified and sequenced to obtain a DNA barcode.
When this was compared to thousands of records of Lepidoptera DNA barcodes stored in the Barcode of Life Data Systems (BOLD) database, it was confirmed that the mescal liquid contained DNA related to the family Cossidae, of which the agave butterfly is a member.
Apart from the mescal, the scientists also successfully amplified and sequenced DNA barcodes from the 95 percent ethanol preservative of 70 freshly collected specimens including whole insects (caddisflies and mayflies) and plant leaves as well as seven archival specimens from diverse phyla that were collected seven to 10 years earlier.
A report on their study entitled "Direct PCR amplification and sequencing of specimens' DNA from preservative ethanol" is published today in the online edition of the journal BioTechniques (www.biotechniques.com). The study is part of the technology development phase of the International Barcode of Life project (iBOL).
"These results suggest that DNA extraction is a superfluous step in many [molecular biology] protocols," the report says. "Preservative ethanol can be used as a source of genetic material for non-invasive sampling or when no tissue
|Contact: John Chenery|
International Barcode of Life