Navigation Links
Mescal worm test shows DNA leaks into preservative liquids
Date:2/9/2010

Guelph, Ontario--Just because you don't swallow the worm at the bottom of a bottle of mescal doesn't mean you have avoided the essential worminess of the potent Mexican liquor, according to scientists from the Biodiversity Institute of Ontario (BIO) at the University of Guelph.

They have discovered that the liquid itself contains the DNA of the agave butterfly caterpillar the famously tasty mescal "worm".

As part of a study to test their theory that DNA from a preserved specimen can leak into its preservative liquid, the BIO team tested a sample of liquid from a bottle of Monte Alban brand mescal. The liquor was found to contain DNA, which they amplified and sequenced to obtain a DNA barcode.

When this was compared to thousands of records of Lepidoptera DNA barcodes stored in the Barcode of Life Data Systems (BOLD) database, it was confirmed that the mescal liquid contained DNA related to the family Cossidae, of which the agave butterfly is a member.

Apart from the mescal, the scientists also successfully amplified and sequenced DNA barcodes from the 95 percent ethanol preservative of 70 freshly collected specimens including whole insects (caddisflies and mayflies) and plant leaves as well as seven archival specimens from diverse phyla that were collected seven to 10 years earlier.

A report on their study entitled "Direct PCR amplification and sequencing of specimens' DNA from preservative ethanol" is published today in the online edition of the journal BioTechniques (www.biotechniques.com). The study is part of the technology development phase of the International Barcode of Life project (iBOL).

"These results suggest that DNA extraction is a superfluous step in many [molecular biology] protocols," the report says. "Preservative ethanol can be used as a source of genetic material for non-invasive sampling or when no tissue specimen is left for further DNA analysis."

The scientists said that the successful identification of the agave caterpillar DNA is "a surprising result" given that mescal is an imperfect preservation medium containing only 40 percent ethanol and many impurities that can degrade DNA. And while they could not obtain a full-length DNA barcode from the mescal, when they repeated the process on the 95 percent ethanol preservatives used for the freshly collected specimens, their success rate was near perfect.

"Showing that the DNA of a preserved specimen can be extracted from the preservative liquid introduces a range of important possibilities," said Dr. Mehrdad Hajibaebi, a member of the research team. "We can develop inexpensive, high-throughput and non-invasive genetic analysis protocols for situations where the original tissue cannot be touched or when there is simply no sample left for analysis."


'/>"/>
Contact: John Chenery
jchenery@uoguelph.ca
519-780-5483
International Barcode of Life
Source:Eurekalert

Related biology technology :

1. 3 strikes, youre out? Refiling of Amendment to Change Constitution to Criminalize Stem Cell Cures Shows Trouble with Anti-Cures Cause
2. New Study Shows Promise for Hydrogen Sulfide in Reducing Heart-Attack Damage, Ikaria Announces
3. International Survey Shows People Over Forty Ignore Risk of Blindness
4. Aidas Rh-Apo2L Clinical Testing Shows Preliminary Efficacy in Helping to Treat Lung Cancer, Stomach Cancer, Lymphoma, Pancreatic Cancer & Kidney Cancer
5. New Oral MEK Inhibitor, RDEA119, Shows Favorable Anti-Tumor Properties
6. Celator(R) Pharmaceuticals Study Shows CPX-571 Maintains Synergistic Ratio of Irinotecan and Cisplatin to Improve Anti-tumor Activity
7. Video shows buckyballs form by shrink wrapping
8. National Survey Shows Most Women Are Not Satisfied With Their Progesterone Treatment
9. Kosans Hsp90 Inhibitor, Tanespimycin, Shows 55% Clinical Benefit in Patients With Trastuzumab-Refractory Metastatic Breast Cancer
10. What To Do When a Holiday Guest Shows Up With a Pet
11. Federally-Funded Study Shows More Women Can Avoid Hysterectomy for Common Problem
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/10/2017)... ... October 10, 2017 , ... ... antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that give it exclusive ... a technology developed in collaboration with Children’s Hospital Los Angeles (CHLA). ...
(Date:10/10/2017)... CRUZ, Calif. , Oct. 10, 2017 ... grant from the NIH to develop RealSeq®-SC (Single Cell), ... kit for profiling small RNAs (including microRNAs) from single ... Analysis Program highlights the need to accelerate development of ... "New techniques for measuring ...
(Date:10/9/2017)... ... October 09, 2017 , ... The award-winning American Farmer television series ... 2018. American Farmer airs Tuesdays at 8:30aET on RFD-TV. , With global population ... challenge of how to continue to feed a growing nation. At the same time, ...
(Date:10/7/2017)... , ... October 06, 2017 ... ... experience providing advanced instruments and applications consulting for microscopy and surface analysis, ... in application consulting, Nanoscience Analytical offers a broad range of contract analysis ...
Breaking Biology Technology:
(Date:5/16/2017)... TEANECK, N.J. , May 16, 2017  Veratad ... leading provider of online age and identity verification solutions, ... the K(NO)W Identity Conference 2017, May 15 thru May ... Ronald Regan Building and International Trade Center. ... across the globe and in today,s quickly evolving digital ...
(Date:5/6/2017)... , May 5, 2017 RAM ... announced a new breakthrough in biometric authentication based ... quantum mechanical properties to perform biometric authentication. These new ... semiconductor material created by Ram Group and its ... entertainment, transportation, supply chains and security. Ram Group ...
(Date:4/19/2017)... 2017 The global military biometrics ... marked by the presence of several large global players. ... five major players - 3M Cogent, NEC Corporation, M2SYS ... nearly 61% of the global military biometric market in ... global military biometrics market boast global presence, which has ...
Breaking Biology News(10 mins):