Navigation Links
JCI online early table of contents: March 6, 2008
Date:3/6/2008

; E-mail: steinma@mail.rockefeller.edu.

View the PDF of this article at: https://www.the-jci.org/article.php?id=34224


EDITOR'S PICK: Genetic manipulation might model cystic fibrosis

Cystic fibrosis (CF) is caused by mutations in the gene responsible for making the protein CFTR. Progress toward understanding the disease-causing mechanisms and developing new therapies has been hampered by the lack of a good animal model; e.g., CF mice fail to develop many of the symptoms seen in patients with CF. However, two independent reports in the April issue of the Journal of Clinical Investigation have described the first steps toward developing large animal (pig and ferret) models of the disease.

A team of researchers from the University of Iowa, Iowa City, and the University of Missouri Columbia, used recombinant adeno-associated virus (rAAV) vectors to alter the CFTR gene in pig fibroblasts such that was disrupted or contained the most common CF-associated mutation (delta-F508). The nucleus of these cells was used to fertilize the eggs of female pigs, and male piglets carrying one or other the mutated CFTR genes were generated. A similar approach rAAV targeting of the CFTR gene in ferret fibroblasts, followed by the transfer of the nucleus of the cells to the eggs of female ferrets was taken by John Engelhardt and colleagues, at the University of Iowa, Iowa City, to generate male ferrets carrying a mutated CFTR gene. As noted by the authors of both papers, these studies have not only provided animals that might lead to the development of a good animal model of CF, but also have described approaches that might be useful for the development of large animals models of other human genetic diseases.

TITLE: Production of CFTR-null and CFTR-delta-F508 heterozygous pigs by adeno-associated virusmediated gene ta
'/>"/>

Contact: Karen Honey
press_releases@the-jci.org
215-573-1850
Journal of Clinical Investigation
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Social Network for Scientists Marks Ten Years Online
2. BioReliance Corporation Launches iNet(TM) for Online Results Tracking of Biological Safety Tests
3. K-State Research Leading to Software to Help Nations Cattle Producers Identify Biosecurity Risks, Evaluate Impact of Cow-Calf Diseases Online
4. Bio Career Center Launches First Online Jobs Board for Life Science PhDs, MDs
5. New Online Help Desk Offers Safe Lifting Assistance to Healthcare Facilities
6. New Online Learning Tool Fills the Knowledge Gap on Botulinum Toxin Type B for Cervical Dystonia
7. Arpida-Supported CME Symposium Available Online
8. Online Career Site for Life Science Professionals Continues Growth
9. Reclast(R) Receives US FDA Approval as First and Only Once-Yearly Treatment for Women With Postmenopausal Osteoporosis
10. New technique can be breakthrough for early cancer diagnosis
11. The Tauri Group LLC Awarded U.S. Department of Homeland Security Chemical and Biological Early Detection Systems Program Office Support Contract
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/29/2015)... , June 29, 2015   Veracyte, Inc . ... the field of molecular cytology, today announced that it ... prestigious Top Workplaces list for the second year in ... based solely on an annual survey of Bay Area-company ... that the degree to which Veracyte values our employees ...
(Date:6/26/2015)... KANSAS CITY, Kan. , June 26, ... (NASDAQ: PETX ), a pet therapeutics ... commercialization of innovative biopharmaceutical products for companion ... pivotal field effectiveness study of capromorelin (AT-002), ... in dogs.  Capromorelin is a small molecule ...
(Date:6/26/2015)... MUMBAI, India , June 26, 2015 ... a leading global IT services, consulting and business solutions ... Leader in Worldwide Life Sciences R&D Risk-Based Monitoring by ... MarketScape: Worldwide Life Science R&D Risk-Based Monitoring 2015 Vendor ... The report evaluated eight leading IT services vendors with ...
(Date:6/26/2015)... 2015 The report "Biolubricants Market ... Metalworking fluids, Chainsaw oils, Mold Release Agents), End Use ... Global trends and Forecasts to 2020" published by MarketsandMarkets, ... by 2020 at a CAGR of 6.27% between 2015 ... Tables and   66 Figures spread through 170 Pages ...
Breaking Biology Technology:Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 2Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 3Aratana Therapeutics Announces Positive Pivotal Results for AT-002 2Aratana Therapeutics Announces Positive Pivotal Results for AT-002 3Aratana Therapeutics Announces Positive Pivotal Results for AT-002 4Aratana Therapeutics Announces Positive Pivotal Results for AT-002 5TCS Recognized as a Leader in Life Sciences R&D Risk-Based Monitoring by IDC MarketScape 2TCS Recognized as a Leader in Life Sciences R&D Risk-Based Monitoring by IDC MarketScape 3TCS Recognized as a Leader in Life Sciences R&D Risk-Based Monitoring by IDC MarketScape 4Biolubricants Market Worth $2,972.13 Million by 2020 2Biolubricants Market Worth $2,972.13 Million by 2020 3
... Application of the RoboCycler Gradient 96 temperature ... , Stratagenes RoboCycler Gradient 96 temperature cycler ... perform fluorescent-tag cycle,sequencing using the ABI PRISM ... Hot Top Assembly eliminates the need for oil or ...
... Allelic discrimination kits with excellent specificity, sensitivity, and,assay ... Lingyu Chen ,Stratagene , Because of their unique ... differences, making them ideal for mutation detection and,allelic ... developed a,series of Mx4000 molecular beacon allelic discrimination ...
... , Douglas Drake Michelle Cayouette Lisa Filippone ,Bob LeSchofs ,Stratagene , , ... , , Figure 1: The ... Stratagene has developed the new SmartPower , ... electrophoretic application. The SmartPower 4000 power supply has been, ...
Cached Biology Technology:Automated Fluorescent-Tag Cycle Sequencing 2Automated Fluorescent-Tag Cycle Sequencing 3Automated Fluorescent-Tag Cycle Sequencing 4Automated Fluorescent-Tag Cycle Sequencing 5Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 2Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 3Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 4Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 5Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 6Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon,Allelic Discrimination Kits 7A Versatile Power Supply for All Electrophoresis Applications 2A Versatile Power Supply for All Electrophoresis Applications 3A Versatile Power Supply for All Electrophoresis Applications 4A Versatile Power Supply for All Electrophoresis Applications 5
(Date:6/24/2015)... Switzerland , June 24, 2015 ... partnership with emerging biometric password solution one face ... one face in, entering ... a ground-breaking and affordable multifactor-authentication biometric answer to ... recognition technology, provided in partnership with KeyLemon, one ...
(Date:6/23/2015)... Fla. , June 23, 2015 /PRNewswire/ ... management and authentication solutions, today announced enhanced functionality ... multi-factor authentication solution.  The enhancements build on ... of the DigitalPersona Altus platform and provide ... compatibility. In today,s environment of ...
(Date:6/17/2015)... and HILDEN, Germany , June 17, 2015 ... Frankfurt Prime Standard: QIA) today launched new Investigator ® ... laboratories in the United States . The ... analyze multiple key genomic markers (short tandem repeats or STRs) ... Sensor to evaluate the quality of DNA in each sample, ...
Breaking Biology News(10 mins):KeyLemon Announces Partnership With Biometric Password Solution onefacein 2Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5
... The Van Andel Research Institute (VARI) and the ... alliance that will enable both to maximize their worldwide ... institutes jointly announced today the initiation of an "alliance ... Rapids, Mich.-based VARI and Phoenix, Ariz.-based TGen will enable ...
... to identify microbes that may cause preterm labor in some ... contractions and an early birth, and look at different groups ... prematurity. The work of seven researchers will be supported ... Prematurity Research Initiative (PRI) grants. The nearly $2.7 million ...
... against respiratory infections, the body typically produces a little fluid ... research suggests that the influenza virus can tip the balance ... supply of oxygen to the rest of the body. ... but this research suggests that it,s not the presence of ...
Cached Biology News:Van Andel Research Institute and TGen undertake alliance to promote science and health 2Van Andel Research Institute and TGen undertake alliance to promote science and health 3March of Dimes provides $2.7 million in new funding for preterm birth research 2March of Dimes provides $2.7 million in new funding for preterm birth research 3Study: Fluid buildup in lungs is part of the damage done by the flu 2Study: Fluid buildup in lungs is part of the damage done by the flu 3
... Amplification Kit, 100 reactions. Prepare circular ... 4-6 h.Generate microgram quantities of template ... material.Use amplified DNA directly for cycle ... or M13 liquid cultures, colonies, plaques, ...
Polypropylene test tube racks, used in Thermo Forma's line of Test Tube Rack holders...
For quantitative determination of Aldolase in Serum (an indicator of muscle damage). Ready-to-use liquid stable reagent. Formulated to eliminate interference from pyruvates. Application sheets are ...
This Universal 24" X 18" (61 X 45.7cm.) shaker platforms will accept any Thermo Forma shaker flask clip....
Biology Products: