Navigation Links
JCI online early table of contents: March 6, 2008
Date:3/6/2008

; E-mail: steinma@mail.rockefeller.edu.

View the PDF of this article at: https://www.the-jci.org/article.php?id=34224


EDITOR'S PICK: Genetic manipulation might model cystic fibrosis

Cystic fibrosis (CF) is caused by mutations in the gene responsible for making the protein CFTR. Progress toward understanding the disease-causing mechanisms and developing new therapies has been hampered by the lack of a good animal model; e.g., CF mice fail to develop many of the symptoms seen in patients with CF. However, two independent reports in the April issue of the Journal of Clinical Investigation have described the first steps toward developing large animal (pig and ferret) models of the disease.

A team of researchers from the University of Iowa, Iowa City, and the University of Missouri Columbia, used recombinant adeno-associated virus (rAAV) vectors to alter the CFTR gene in pig fibroblasts such that was disrupted or contained the most common CF-associated mutation (delta-F508). The nucleus of these cells was used to fertilize the eggs of female pigs, and male piglets carrying one or other the mutated CFTR genes were generated. A similar approach rAAV targeting of the CFTR gene in ferret fibroblasts, followed by the transfer of the nucleus of the cells to the eggs of female ferrets was taken by John Engelhardt and colleagues, at the University of Iowa, Iowa City, to generate male ferrets carrying a mutated CFTR gene. As noted by the authors of both papers, these studies have not only provided animals that might lead to the development of a good animal model of CF, but also have described approaches that might be useful for the development of large animals models of other human genetic diseases.

TITLE: Production of CFTR-null and CFTR-delta-F508 heterozygous pigs by adeno-associated virusmediated gene ta
'/>"/>

Contact: Karen Honey
press_releases@the-jci.org
215-573-1850
Journal of Clinical Investigation
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Social Network for Scientists Marks Ten Years Online
2. BioReliance Corporation Launches iNet(TM) for Online Results Tracking of Biological Safety Tests
3. K-State Research Leading to Software to Help Nations Cattle Producers Identify Biosecurity Risks, Evaluate Impact of Cow-Calf Diseases Online
4. Bio Career Center Launches First Online Jobs Board for Life Science PhDs, MDs
5. New Online Help Desk Offers Safe Lifting Assistance to Healthcare Facilities
6. New Online Learning Tool Fills the Knowledge Gap on Botulinum Toxin Type B for Cervical Dystonia
7. Arpida-Supported CME Symposium Available Online
8. Online Career Site for Life Science Professionals Continues Growth
9. Reclast(R) Receives US FDA Approval as First and Only Once-Yearly Treatment for Women With Postmenopausal Osteoporosis
10. New technique can be breakthrough for early cancer diagnosis
11. The Tauri Group LLC Awarded U.S. Department of Homeland Security Chemical and Biological Early Detection Systems Program Office Support Contract
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/28/2015)... 2015 Research and Markets( http://www.researchandmarkets.com/research/6cg3zw/cell_therapy ) ... report "Cell Therapy - Technologies, Markets and ... report describes and evaluates cell therapy technologies and ... important role in the practice of medicine. Hematopoietic ... bone marrow transplants. Role of cells in drug ...
(Date:5/28/2015)... CARLOS, Calif. , May 28, 2015 /PRNewswire/ ... testing, today announced a study published in ... the Panorama single-nucleotide polymorphism (SNP)-based non-invasive prenatal test ... the first trimester. A study ... that complete molar pregnancies occur in about 1 ...
(Date:5/28/2015)... , May 28, 2015 RXi Pharmaceuticals Corporation ... discovering and developing innovative therapeutics, primarily in the areas ... a public offering of 26 million units at a ... gross proceeds of approximately $10.4 million. Each unit consists ... 13-month overallotment purchase right to purchase 0.50 of a ...
(Date:5/28/2015)... 2015 The Fertility Centers of New ... Danielle Vitiello has taken on a new and expanded ... will provide guidance, leadership, oversight and quality assurance for ... Vitiello among our team of experienced reproductive endocrinologists,” said ... A. Hill, M.D. “Her expertise and compassionate, individualized care ...
Breaking Biology Technology:Global Cell Therapy Market 2015-2020 - Technologies, Markets and Companies 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 3RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2
... MedCap press releases contain false and misleading ... 25 /PRNewswire-FirstCall/-- VaxGen,Inc. (OTC Bulletin Board: VXGN.OB), ... ), Dear Stockholders, On March ... stockholder in,VaxGen, issued a press release entitled ...
... BEIJING, March 25 /Xinhua-PRNewswire/ -- Sinovac Biotech Ltd. (Amex:,SVA), ... that its,Annual General Meeting originally scheduled for March 25, ... the March 25, 2008 Annual General Meeting, the required ... present. In accordance with,Antigua law, the meeting is adjourned ...
... N.J., March 24 Osteotech, Inc.,(Nasdaq: OSTE ... biologic solutions for,regenerative medicine, announced today that the ... validity of its U.S. Patent No.,5,676,146. The ,146 ... demineralized bone, with a radiopaque material, such as ...
Cached Biology Technology:VaxGen Issues Rebuttal to MedCap Letter 2VaxGen Issues Rebuttal to MedCap Letter 3VaxGen Issues Rebuttal to MedCap Letter 4VaxGen Issues Rebuttal to MedCap Letter 5VaxGen Issues Rebuttal to MedCap Letter 6VaxGen Issues Rebuttal to MedCap Letter 7VaxGen Issues Rebuttal to MedCap Letter 8VaxGen Issues Rebuttal to MedCap Letter 9VaxGen Issues Rebuttal to MedCap Letter 10VaxGen Issues Rebuttal to MedCap Letter 11Sinovac Announces Adjournment of Annual General Meeting 2U.S. Patent Office Confirms Validity of Osteotech's U.S. Patent No. 5,676,146 2
(Date:5/21/2015)... 21, 2015 According to ... Solutions (Hardware, Software, Services), by Applications (Surveillance (Airborne, ... by End-Users (Military & Defense & Commercial) - ... Imaging Market is expected to grow from $7252.0 ... at a Compound Annual Growth Rate (CAGR) of ...
(Date:5/20/2015)... 20, 2015 ... announced the addition of the  "5-year ... Recognition Market"  report to their offering.  ... service analyses trends in the iris ... commercial sectors, globally. Despite hardware pricing ...
(Date:5/19/2015)... May 18, 2015 Fingerprint Cards (FPC) ... FPC1025, FPC1145, FPC1155 and FPC1035 from one if its module ... and including Q3 2015 and the sensors will be used ... order values for 2015 hereby amount to 740 MSEK to ... of 140 MSEK and a number of smaller orders not ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2
... research team has developed a process that removes a ... team has programmed a photosynthetic microbe to self-destruct, making ... potentially less costly. "The real costs involved ... turning them into fuel," said Roy Curtiss, director of ...
... , TORONTO, Dec. 7 ... the assets of the healthcare communications company previously operating as ... move forward under new leadership and funding. , IgeaCare Solutions ... Chief Executive Officer. Under his direction, the new company will ...
... air and water pollution along the Athabasca River and ... say pollution levels have increased as a direct result ... biological sciences professor David Schindler was part of the ... study and found high levels of Polycyclic Aromatic Compounds. ...
Cached Biology News:Self-destructing bacteria improve renewable biofuel production 2Self-destructing bacteria improve renewable biofuel production 3IgeaCare Solutions Inc. Emerges Under New Leadership and Funding 2
... PDS-1000 He biolistic system enables 7-10 times more ... It fits into the shocking chamber of the ... shock wave over 7 microcarriers. By uniformly spreading ... larger area, the system maximizes the number of ...
Kit Components: Anti-Acetylated Histone H3 Salmon Sperm DNA/Protein A Agarose All necessary buffers. ...
... mitochondrial transmembrane potential is one of ... following induction of apoptosis. The ... lipophilic cation, termed as MitoLight, as ... a mitochondrial dye that stains mitochondria ...
Liquid. 50 μg/ml in PBS, pH 7.4. PROTECT FROM LIGHT. A convenient form of propidium iodide useful for flow cytometry studies. RTECS SF7949600, CAS 25535-16-4, M.W. 668.4. ...
Biology Products: