Navigation Links
Investigating how spiders spin their silk, researchers unravel a key step
Date:5/12/2010

ecial storage configuration.

X-ray structure analysis, which is so successful in other domains, was of little help here, since it can only be used to analyze crystals. And up to the instant in which the solid silk fiber is formed, everything takes place in solution. The method of choice was therefore nuclear magnetic resonance spectroscopy (NMR). Using the equipment of the Bavarian NMR Center, Franz Hagn, a biochemist from Horst Kessler's work group at the Institute for Advanced Study (TUM-IAS) at the TU Muenchen, managed to unravel the structure of a control element responsible for the formation of the solid fiber. Now the researchers could, together with Lukas Eisoldt and John Hardy from Thomas Scheibel's group, shed light on this control element's mode of operation.

"Under storage conditions in the silk gland these control domains are connected pair-wise in such a way that the interlinking areas of both chains can not lie parallel to each other," Thomas Scheibel explains. "Interlinking is thus effectively prevented." The protein chains are stored with the polar areas on the outside and the hydrophobic parts of the chain on the inside, ensuring good solubility in the aqueous environment.

When the protected proteins enter the spinning duct, they encounter an environment with an entirely different salt concentration and composition. This renders two salt bridges of the control domain unstable, and the chain can unfold. Furthermore, the flow in the narrow spinning duct results in strong shear forces. The long protein chains are aligned in parallel, thus placing the areas responsible for interlinking side by side. The stable spider silk fiber is formed.

"Our results have shown that the molecular switch we discovered at the C-terminal end of the protein chain is decisive, both for safe storage and for the fiber formation process," says Franz Hagn. An important foundation for these results was established through cooperation of Thomas
'/>"/>

Contact: Andreas Battenberg
battenberg@zv.tum.de
49-892-891-0510
Technische Universitaet Muenchen
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. The Multiple Myeloma Research Consortium (MMRC) and Proteolix Initiate Phase 2 Clinical Trial Investigating Carfilzomib for the Treatment of Multiple Myeloma
2. Oncolytics Biotech Inc. Announces Approval for U.K. Clinical Trial Investigating REOLYSIN(R) in Combination with Cyclophosphamide
3. Hagens Berman Sobol Shapiro Investigating CellCyte
4. Oncolytics Biotech Inc. Announces U.K. Phase II Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin
5. Oncolytics Biotech Inc. Starts Patient Enrolment in U.K. Phase II Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin
6. Oncolytics Biotech Inc. Announces U.S. Phase 2 Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin
7. Oncolytics Biotech Inc. Starts Patient Enrolment in U.S. Phase 2 Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin
8. Innocoll Announces Provisional Findings From Its Three Phase 2 Clinical Trials Investigating CollaRx(R) GENTAMICIN TOPICAL for the Treatment and Prevention of Diabetic Foot Infections
9. New Survey Reveals Many Adults With High Cholesterol Fail to Take Necessary Steps to Improve Their Condition
10. Actress Aida Turturro Tells Americans to Start Now to Talk With Their Healthcare Provider About Taking Control of Their Diabetes
11. National Survey Shows Most Women Are Not Satisfied With Their Progesterone Treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/29/2015)... Translation is frequently listed as one of the ... used in a way that is right for a company, ... product or service offering. , Here are some of ... on a company's bottom line: , 1.    Attract bigger audience. , ... can use, why not offer it globally? Nelson Mandela once ...
(Date:5/28/2015)... 28, 2015 Research ... the addition of Jain PharmaBiotech,s new report ... and Companies" to their offering. ... This report deals with therapeutic drug monitoring, ... patient care by monitoring drug levels in ...
(Date:5/28/2015)... , May 28, 2015 ... ) has announced the addition of ... - Technologies, Markets and Companies"  to ... ,This report describes and evaluates animal ... medicine and pharmaceuticals as well as ...
(Date:5/28/2015)... Research and Markets( http://www.researchandmarkets.com/research/l9dqwt/animal_genetics ) has announced ... Products, by Testing services - Global Forecast to 2020" ... animal genetics market is estimated to be worth around ... of 8-9% during the forecast period of 2014 to ... increasing animal protein consumption, increasing adoption of advanced genetic ...
Breaking Biology Technology:Three Ways Translation Can Help Businesses Grow 2Global Therapeutic Drug Monitoring Market 2015-2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 3Global Animal Genetics (Canine, Equine, Poultry, Porcine, Bovine, and Genetic Material) Market 2015 - Global Forecast to 2020 2
... Ohio, Oct. 18, 2011 Minimally Invasive Devices, Inc ... new product, FloShield PLUS , utilizing the novel Flo-X ... of the end of the laparoscope without removal from the ... MID,s FloShield laparoscopic visualization system. In the first 19 FloShield ...
... Oct. 18, 2011 Ikaria, Inc., a critical care ... to address the significant needs of critically ill patients ... its Chairman and CEO, Daniel Tasse, will present at ... Tasse,s presentation will be given on Tuesday, October 25, ...
... GenVec, Inc. (NASDAQ: GNVC ) today announced ... present a Company overview highlighting recent developments at the 2011 ... at 3:00 p.m. PDT at the Palace Hotel in San ... will be available both live and on replay. The presentation ...
Cached Biology Technology:Ikaria® to Present at 2011 BIO Investor Forum 2GenVec to Present at the 2011 10th Annual BIO Investor Forum 2
(Date:5/18/2015)... Fingerprint Cards (FPC) has received an ... and FPC1035 from one if its module partners. Deliveries are ... 2015 and the sensors will be used by smartphone manufacturers ... 2015 hereby amount to 740 MSEK to date. In addition ... and a number of smaller orders not separately communicated amounting ...
(Date:5/11/2015)... , May 11, 2015  Synaptics Incorporated (NASDAQ: ... interface solutions, today announced the appointment of ... Chief Financial Officer, reporting to Rick Bergman ... current Chief Financial Officer, Kathleen Bayless , ... Mr. Ali brings extensive financial management ...
(Date:5/10/2015)... Fingerprint Cards (FPC) has received an ... the distributor World Peace Industrial Group (WPI), part of WPG ... Asia . Deliveries are planned to take place during ... manufacturers in China . The order value ... of + 1 000 MSEK for 2015.   Jörgen ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2
... virus as for example HIV and bird flu able to ... purpose of the virus? Researchers at the University of Copenhagen ... a collaboration between molecular biologists and physicists. ”The molecular biologists ... interplay between the intercellular parts, while the physicists have the ...
... testosterone levels of healthy men decline as they get older. ... objectively measured differences in the amount of sleep a healthy ... the morning, according to a study published in the April ... by Plamen Penev, MD, PhD, of the University of Chicago, ...
... in the spinal cord of a larval zebrafish, Cornell ... that regulates the speed of the fish’s movement. The ... spinal cords and Parkinson’s disease, where movements slow down ... of Recruitment in Spinal Cord," published in the March ...
Cached Biology News:Danish researches solve virus puzzle 2Fast and slow -- How the spinal cord controls the speed of movement 2
Request Info...
... the secretion of cytoplasmic granules, is ... response of leukocytes (e.g. basophils, neutrophils, ... to histamine, these secretory granules contain ... chemokines, and many proteases (Hallgren, 2001). ...
... The S&S Custom Processing Service enables scientists who ... and data analysis software to have their biological ... 16 analytes. Its as easy as 1-2-3 ... quantified in your samples from the S&S antibody ...
... stain has bright fluorescence when bound to dsDNA ... for detecting dsDNA in gels using laser scanners ... nucleic gel acid stain has also found ... bandshift assays. One mL stains 100 minigels. A ...
Biology Products: