Navigation Links
If oxygen becomes the undoing of proteins
Date:10/12/2011

red by the BMBF, it was possible to show the diverse phases of the inactivation process for the first time using the so-called X-ray absorption spectroscopy. The researchers used the synchroton radiation source Swiss Light Source in Switzerland for this specific type of measurement. It generates particularly strong rays, thus enabling the characterization of metal centers in proteins. Amongst other things, the scientists thus determined the chemical nature of the iron centers and the distance from the surrounding atoms using atomic resolution.

Inactivation in three phases

The team of researchers from Bochum and Berlin used a new experimental procedure. They initially brought the hydrogenase sample into contact with oxygen for a few seconds to minutes and finally for a couple of hours and then suppressed all proceeding reactions by deep-freezing it in liquid nitrogen. The subsequently gained spectroscopic data was used for the development of a model for a three-phase inactivation process. According to this model, an oxygen molecule initially binds to the di-iron center of the hydrogenase, which leads to the development of an aggressive oxygen species. In the subsequent phase, this attacks and modifies the four-iron center. During the final phase, further oxygen molecules bind and the entire complex disintegrates. "The entire process thus consists of a number of consecutive reactions that are distinctly separated in time", says Lambertz. "The velocity of the entire process is possibly dependent on the phase during which the aggressive oxygen species moves from the di-iron to the four-iron center. We are currently elaborating further experiments to investigate this."


'/>"/>
Contact: Camilla Lambertz
Camilla.Lambertz@rub.de
49-234-322-4496
Ruhr-University Bochum
Source:Eurekalert

Page: 1 2

Related biology technology :

1. Oxygen Healthcare (O2h) Announces Expanded Chemistry Collaboration With Heptares Therapeutics
2. Sangart, Inc. Reports Positive Phase IIa Data for Novel Oxygen Therapeutic Agent MP4OX in Severe Trauma Patients
3. Dehaier Medical Systems Signs $1.6 Million Oxygen-Chip Sales Contract
4. Medical Simulation Corporation Partners with Hutchinson Technology in Training of Healthcare Professionals on Noninvasive Tissue Oxygenation Monitoring
5. Heptares Therapeutics Extends Multi-FTE Chemistry Collaboration With Oxygen Healthcare (O2h)
6. Oxygen Biotherapeutics, Inc. Outlines Plans for New Products During Investor Conference Presentation
7. Spectros Corp. Deep-Monitoring Continuum Noninvasive Cerebral Oxygenation Sensors
8. Oxygen Biotherapeutics, Inc. Expands Board of Directors
9. New Oxygen Biotherapeutics, Inc. Investor Presentation Available on Company Website
10. Oxygen Biotherapeutics, Inc. Seeks Shareholder Approval for Reverse Stock Split
11. Oxygen Biotherapeutics, Inc. to Present at Investment Conferences in New York and Zurich
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/15/2014)... RedBrick Health , a fast-growing leader in consumer health ... EmblemHealth , the New York -based ... RedBrick Compass health assessment, combined with the RedBrick Journeys ... EmblemHealth is among the first health plans in the ...
(Date:1/15/2014)... A study has been launched to test ... track could help to tackle the problem of obesity.  This ... GP surgery based in Stowmarket) and academics at University Campus ... Telemetry technology, which is inspired by equipment used to collect ...
(Date:1/15/2014)... Two champions of science, technology, engineering, and ... annual competition for middle and high school students designed ... study. The competition presents students with real-world problems experienced ... Mathematics, and Sciences is a program administered by ...
(Date:1/15/2014)... January 15, 2014 DTS Language Services, ... Online Web Portal for Life Science organizations who need document ... the subject matter of their documents in advance with a ... reduce time-to-delivery of translations, often a critical factor in clinical ...
Breaking Biology Technology:EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5DTS Improves Efficiency for Life Science Document Translations 2
... Proteon Therapeutics,( http://www.proteontherapeutics.com ), a privately ... address the,medical needs of patients with kidney ... Annual Meeting of the American Society of ... Dilates Outflow,Veins in a Preclinical Model of ...
... Dr. Brad Thompson, President,and CEO of Oncolytics Biotech Inc. ... the Company at the Acumen BioFin Rodman & Renshaw ... 3:20 p.m. ET.,The event will be held at the ... live audio webcast of the presentation will be available ...
... agency to provide free home caregiving to disabled ... has announced its,2007 We Care For Veterans campaign ... services to qualified disabled veterans. (Logo: ... the nation are,donating 20 hours each of free ...
Cached Biology Technology:Proteon Therapeutics to Present at Annual Meeting of American Society of Nephrology in San Francisco on November 2, 2007 2Media Advisory - Oncolytics Biotech Inc. to Present at Acumen BioFin Rodman & Renshaw 9th Annual Healthcare Conference 2Homewatch CareGivers Launches 2007 We Care For Veterans Campaign 2
(Date:4/23/2014)... evidence of an interaction between prenatal smoking and ... children, especially in girls. , "The interesting issue ... smoking will have behavioral problems. Some might, ... Professor at Sam Houston State University, College of ... "One possible explanation for this is that ...
(Date:4/23/2014)... turning away from using triclosan as an antimicrobial ingredient ... And now scientists are reporting new evidence that appears ... ACS journal Chemical Research in Toxicology , found ... octylphenol, promoted the growth of human breast cancer cells ... , Kyung-Chul Choi and colleagues note that hormonal imbalances ...
(Date:4/23/2014)... date of the family of bacteria that causes ... and reveals surprising findings about the bacteria,s origin ... health strategies to control this respiratory disease, which ... analysis of 343 strains of the Bordetella ... over the last 100 years illustrates how vaccination ...
Breaking Biology News(10 mins):Genetics risk, prenatal smoking may predict behavioral problems 2In lab tests, the antimicrobial ingredient triclosan spurs growth of breast cancer cells 2Impact of whooping cough vaccination revealed 2
... Institute of Science and his colleagues caused a storm ... years back that tiny airborne particles, known as aerosols, ... change ?having, on a local scale, an even greater ... how these particles influence clouds have generated many uncertainties. ...
... sequencing of the chimpanzee genome last year, geneticists have spent ... closest evolutionary relative, looking for the differences that distinguish the ... human DNA sequence with the most dramatically increased rate of ... still unknown, but it appears to be directly involved in ...
... new study, published today in the current issue ... Sciences has significant implications for the design of ... in human biomedical research. The groundbreaking study, entitled, ... Quantum Dots", demonstrates that quantum dots can one ...
Cached Biology News:Tiny airborne particles are a major cause of climate change 2Tiny airborne particles are a major cause of climate change 3Researchers identify human DNA on the fast track 2Researchers identify human DNA on the fast track 3Connect the Quantum Dots 2
... filter (S37100, Photographic Filters for Fluorescent ... for black-and-white photography of gels stained ... stain, the safer ethidium bromide alternative. ... filter is identical to the SYPRO ...
Enodgenous alkaline phosphotase inhibitor. Levamisole solution is supplied in concentrated form (20x). The final concentration of levamisole in the substrate solution will be 1 mM....
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain....
Standard 2" or 3" Plastic can be ordered with 81 or 100 cell dividers. Mini 2" Plastics Boxes come standard with 25 cell dividers. Plastic boxes can also be ordered with corresponding freezer racks...
Biology Products: