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IROA Technologies automates metabolic profiling with launch of labeling and software kits
Date:12/11/2013

ANN ARBOR, Mich., Dec. 11, 2013 /PRNewswire/ -- IROA® is a novel metabolite labeling tool which enables the next-generation metabolomics IROA ClusterFinder™ data analysis software to eliminate artifacts, and find, identify and quantitate biochemical compounds of interest. IROA Biochemical Quantitation Series 100 and 200 kits make it easy for researchers to analyze biochemicals in bacteria and yeast when combined with sample preparation and mass spectrometry. Designed to quantitate metabolic differences between two groups of cell populations, the kits include uniquely stably-labeled carbon sources provided with either YNB or Minimal media. 

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Instead of using 99% 13C-label, in an IROA protocol the control and experimental samples are isotopically labeled with 95 and 5% 13C (respectively), and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis. This labeling strategy provides several advantages over conventional approaches:  1) compounds arising from biosynthesis (experimental and control) have a unique isotopic signature and all unsigned artifactual signals can be removed from the dataset (usually the largest portion of the dataset); 2) sample-to-sample variance from sample extraction and preparation is minimized because the control and experiment are combined into a single sample; 3) measurement of both the molecular weight and the exact number of carbon atoms in each molecule provides extremely accurate mo
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