Navigation Links
How now, inside the cow: Nearly 30,000 novel enzymes for biofuel production improvements
Date:1/27/2011

bial symbionts located in the foregut perform their magic. To better control the process, Hess and his colleagues at the University of Illinois worked with the fistulated cow model that, for scientific research purposes, allows direct access through a tube into the foregut, which can be considered as a fermentation chamber in which oxygen is absent. Instead of feeding the grasses directly to the cows, the switchgrass samples were placed in nylon bags and then inserted into the cow rumen, where they were left to be digested. After 72 hours, the bags were removed, and the DNAs from the microbes involved in digesting the material that were adherent to the switchgrass were isolated and then sequenced.

The amount of data generated for this study of rumen microbes, 270 billion letters of the DNA code, was enormous; about 100-fold greater than the number of letters in the entire human genome. Generating the data was not the most challenging part of this project, Hess noted. "The real challenge was to analyze the vast amount of data for which no reference genome was available and to identify and produce full-size functional enzymes based solely on information obtained from billions and billions of short snippets of DNA sequences," he said.

To analyze the information, the researchers developed a genome assembly strategy that could handle the vast amount of data while making sure to avoid misassemblies that would have led to chimeras artificial genes not present in the microbial community. "Imagine someone mixing hundreds of jigsaw puzzles with millions of pieces each into a big pile," said Sczyrba. "We tried to put as many of these back together, making as few mistakes as possible. It is not an easy problem. You need a good strategy and a lot of computational resources to solve this problem.

By employing different filters Sczyrba whittled down the number of the more than two million predicted genes to 27,755 candidate genes that encoded a spe
'/>"/>

Contact: David Gilbert
degilbert@lbl.gov
925-296-5643
DOE/Joint Genome Institute
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology technology :

1. New nanoscale transistors allow sensitive probing inside cells
2. Researchers take the inside route to halt bleeding
3. Intelligence inside metal components
4. Researchers develop new way to see single RNA molecules inside living cells
5. Horizon Therapeutics Selected to Present at InsideVentures Debut Investor Conference March 25-26, Santa Barbara, California
6. Stem Cell Industry Insider Mercedes Walton Discusses President Obamas Stem Cell Executive Order
7. Targeting Metastatic Cancer From the Inside: Epeius Biotech Reveals a New Generation of Tools for Medical Gene Delivery
8. Reimbursement Rate Nearly Tripled for Cylexs ImmuKnow® Assay
9. NOVAVAX Awarded Nearly $1 Million in Grants from IRS Under Qualifying Therapeutic Discovery Project
10. Combined Sales of Seven Emerging Therapies for Breast Cancer, Including Drugs From Sanofi-Aventis and AstraZeneca, Will Total Nearly $5 Billion by 2019
11. Texcel Medical Expands Medical Device Development & Manufacturing Capabilities With New Personnel, Nearly $1 Million in Capital Investments
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
How now, inside the cow: Nearly 30,000 novel enzymes for biofuel production improvements
(Date:8/29/2014)... New York (PRWEB) August 29, 2014 According ... “Global Orthobiologics Market” the global orthobiologics market was valued at ... a market worth USD 5,519.9million in 2019at a CAGR of ... population aged 50 years and above is afflicted by bone ... double by the year 2020. This has and will continue ...
(Date:8/29/2014)... Burnt Hills, NY (PRWEB) August 29, 2014 ... at Burnt Hills Veterinary Hospital are seeking candidates to ... for dogs with osteoarthritis. The ultimate goal of ... of donor stem cells into one or two arthritically ... the treated joints. , Candidates for the current ...
(Date:8/29/2014)... 29, 2014 A major challenge before ... start-up dense realm of Boston-Cambridge, is gaining visibility that ... James Sherley, the Director of Boston’s Adult Stem Cell ... and national visibility of his company an important priority ... In addition to a social media marketing campaign ...
(Date:8/28/2014)... 2014 Best Sanitizers, Inc., a leader ... processing industry, is asking industry professionals to prepare for ... the E2 soap they’re currently using to the Best ... Hand hygiene is critical to fighting cross-contamination and ... Six key criteria are identified to evaluate the effectiveness ...
Breaking Biology Technology:Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 2Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 3Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Best Sanitizers, Inc. Asks Food Industry Professionals: With Fall Harvest On the Way, Is Your E2 Hand Soap Up to the Task? 2
... . - Merge Healthcare , a provider of ... designed to overcome challenges posed by the fusing of ... several product upgrades this year as it attempts to ... product accommodates standard image reading methods obtained by PET ...
... year away, campaigns are hustling to build momentum and ... are using every available technology to make their hearts ... election cycle may be the most democratic in modern ... sifting through all that content will be a challenge ...
... saw its largest single-year job increase in recent years, and ... Greater Madison Area Directory of High-Tech Companies published by ... Purchase online The 21st annual directory, which will ... clusters. They reported more than $5.5 billion in combined revenue ...
Cached Biology Technology:Tired of the spin? ExpertVoter website makes candidate selection easier 2Tired of the spin? ExpertVoter website makes candidate selection easier 3Directory shows high-tech growth spurt in Dane Co. 2Directory shows high-tech growth spurt in Dane Co. 3Directory shows high-tech growth spurt in Dane Co. 4
(Date:8/28/2014)... Athens, Ga. The support of peer groups ... effective breastfeeding programs, according to recent University of ... mothers in the Athens-Clarke County area determined that ... the outcomes of mothers of infants. , "Mothers ... be successful at breastfeeding," said study co-author Alex ...
(Date:8/28/2014)... Advocate and Senior Staff Attorney at the Electronic Frontier ... of biometric and mobile commerce experts set to speak ... Tampa, Florida on September 15, ... Rahman, Director, Technology and Strategy at Samsung, and Philippe de ... this year,s event is Mobility at the Crossroads of ...
(Date:8/28/2014)... MD FASEB MARC (Maximizing Access to Research ... for the American College of Sports Medicine,s Conference ... 2014 in Miami, Florida. These awards are ... doctorates and scientists from underrepresented groups into the ... encourage the participation of young scientists at the ...
Breaking Biology News(10 mins):Breastfeeding study shows need for effective peer counseling programs 2Biometrics UnPlugged Welcomes the Electronic Frontier Foundation's Jennifer Lynch to the Mobility at the Crossroads of Commerce and Privacy Summit 2
... demonstrated that our brains fend off distractions. If we are ... are tired, we can no longer do this. , ... remember useful things. Distracting information is refused and neutral information ... longer seems to work. , Test ...
... a good thing (nutrients or water) actually decreases the ... the productivity of a few species, according to a ... , The reduction in species diversity occurs because increasing ... water, makes an ecosystem more homogeneous and consequently reduces ...
... - the idea of using genetic instructions rather than drugs ... but is not yet a viable therapeutic method. One ... place at the right time. , Engineers at the University ... problem. David M. Lynn and his colleagues have created ...
Cached Biology News:Molecular tools make the cut 2Too much of a good thing? Excess nutrients or water limit biodiversity 2Ultrathin films deliver DNA as possible gene therapy tool 2Ultrathin films deliver DNA as possible gene therapy tool 3
... cDNA Library Panels were designed for full-length ... of PCRs to identify the desired cDNA ... in a 96-well "Master Plate," where each ... Having identified the positive well(s) by gel ...
... acid acceptor activity using aminoacyl-tRNA synthetase and a ... Lys, Pro, Thr, Val, Arg, Glu, His, Leu, ... will yield an A 260 of 1.0 ... path). Physical Form: lyophilized powder ...
... use as a carrier in ... Physical form: Solution in 10 ... 1 mM EDTA Preparation Note: ... Concentration: 10 mg/mL ...
... Cleland’s Reagent, Immobilized White solid. ... form of DTT on polyacrylamide resin with applications ... 233153 , 233156 , and ... no soluble components are added to the system. ...
Biology Products: