Navigation Links
Highly lethal Ebola virus has diagnostic Achilles' heel for biothreat detection, scientists say
Date:4/5/2013

By screening a library of a billion llama antibodies on live Ebola viruses in the Texas Biomedical Research Institute's highest biocontainment laboratory, scientists in San Antonio have identified a potential weakness in the make-up of these deadly agents that can immediately yield a sensitive test.

"Detecting single viral protein components can be challenging, especially at very low levels. However, most viruses are repetitive assemblies of a few components, called antigens, with some existing as polymers which present highly 'avid' targets for antibodies," said Texas Biomed virologist Andrew Hayhurst, Ph.D.

"Think of one pair of microscopic Velcro hooks where one hook is the viral antigen and the other is the antibody and it is a weak interaction. Have a thousand pairs of hooks and it makes a very powerful interaction just like Velcro fasteners on hiking gear," Hayhurst explained.

The screening performed by Hayhurst and assistant Laura Jo Sherwood guided the selection of llama antibodies recognizing a polymer hiding within Ebola called nucleoprotein (NP). Remarkably, each antibody could be used in its own right to form a sensitive test for the Ebola NP, whereas most tests would require two different antibodies driving up costs and characterization times.

This research -- funded by National Institutes of Health (NIH), Defense Threat Reduction Agency Basic Science Program/Office of Naval Research and the Texas Biomedical Research Institute -- was published today in the journal PLOS ONE.

"Ebola NP is rather like a cob of corn displaying hundreds of kernels linked in a repetitive polymer, giving us the perfect molecular magnet to attract llama antibodies that can be assembled into highly avid assays based on a single antibody," Hayhurst said.

"Intriguingly, while using one antibody to polymers and aggregates has been put to use in neurodegenerative disease diagnostics for Parkinson's, Alzheimer's and other disorders, it has lagged behind in emerging viral diagnostics. We showcase its simplicity and effectiveness for viral threat detection here and it may well be useful for detecting other emerging viruses."


'/>"/>

Contact: Joseph Carey
jcarey@txbiomed.org
210-258-9437
Texas Biomedical Research Institute
Source:Eurekalert

Related biology technology :

1. Highly efficient production of advanced biofuel by metabolically engineered microorganism
2. Researchers create highly conductive and elastic conductors using silver nanowires
3. Stanford engineers perfecting carbon nanotubes for highly energy-efficient computing
4. UCLA team develops highly efficient method for creating flexible, transparent electrodes
5. Ebola antibody treatment, produced in plants, protects monkeys from lethal disease
6. DiaCarta Inc. Receives CE Mark for QuantiVirus® HPV E6/E7 mRNA Assay
7. Acne Treatment Brand, Probiotic Action, Comments on the Use of a Virus to Treat Acne
8. New Norovirus "Vomiting Virus" Strain Spreads Like Wildfire, My Cleaning Products Publishes Green Guide to Prevent Norovirus Outbreaks in One's Home
9. Viruses cooperate or conquer to cause maximum destruction
10. Inovio Pharmaceuticals Cytomegalovirus (CMV) Synthetic Vaccine Constructs Generate Strong and Broad T-Cell Responses in Preclinical Study
11. When parasites catch viruses
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/11/2017)... ... 2017 , ... At its national board meeting in North ... the co-founder, CEO and chief research scientist of Minnesota-based Advanced Space Technology and ... ARCS Alumni Hall of Fame . ASTER Labs is a technology development ...
(Date:10/11/2017)... Palo Alto, CA, USA (PRWEB) , ... October 11, 2017 , ... ... is set to take place on 7th and 8th June 2018 in San Francisco, ... and policy influencers as well as several distinguished CEOs, board directors and government officials ...
(Date:10/11/2017)... the Netherlands and LAGUNA HILLS, Calif. ... The Institute of Cancer Research, London ... use MMprofilerâ„¢ with SKY92, SkylineDx,s prognostic tool to risk-stratify patients ... trial known as MUK nine . The University of ... trial, which is partly funded by Myeloma UK, and ICR ...
(Date:10/11/2017)... Florida (PRWEB) , ... October 11, 2017 , ... ... Drug Administration (FDA) has granted orphan drug designation to SBT-100, its novel anti-STAT3 ... for the treatment of osteosarcoma. SBT-100 is able to cross the cell membrane ...
Breaking Biology Technology:
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. (NASDAQ: ... announces the filing of its 2016 Annual Report on Form 10-K ... Commission. ... 10-K is available in the Investor Relations section of the Company,s ... the SEC,s website at http://www.sec.gov . 2016 Year ...
(Date:4/11/2017)... 11, 2017 Crossmatch®, a globally-recognized leader ... today announced that it has been awarded a ... Activity (IARPA) to develop next-generation Presentation Attack Detection ... "Innovation has been a driving force within Crossmatch ... allow us to innovate and develop new technologies ...
(Date:4/11/2017)... 2017 NXT-ID, Inc. (NASDAQ:   NXTD ... the appointment of independent Directors Mr. Robin D. Richards ... of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief ... to their guidance and benefiting from their considerable expertise as ...
Breaking Biology News(10 mins):