Navigation Links
HeliScopeCAGE: A new gene expression analysis technique on a single molecule sequencer
Date:5/18/2011

A new gene expression technique adapted for single molecule sequencing has enabled researchers at the RIKEN Omics Science Center (OSC) to accurately and quantitatively measure gene expression levels using only 100 nanograms of total RNA. The technique, which pairs RIKEN's Cap Analysis of Gene Expression (CAGE) protocol with the Helicos Genetic Analysis System developed by Helicos BioSciences Corporation, opens the door to the detailed analysis of gene expression networks and rare cell populations.

In recent years, next-generation DNA sequencers have produced an increasingly detailed picture of how genes are expressed at the molecular level. The transcriptional output of these genes the RNA copies produced from DNA has revealed a richness of complexity in transcript structure and function, providing insights into the molecular-level properties of cancers and other diseases.

One of the most powerful methods for analyzing RNA transcripts is the Cap Analysis of Gene Expression (CAGE) protocol developed at the RIKEN OSC. A unique approach, CAGE enables not only high-throughput gene expression profiling, but also simultaneous identification of transcriptional start sites (TSS) specific to each tissue, cell or condition.

With HeliScopeCAGE, the OSC research team has adapted the existing CAGE protocol for use with the revolutionary HeliScopeTM Single Molecule Sequencer. Unlike earlier sequencers, the HeliScope Sequencer does not employ polymerase chain reaction (PCR) amplification to multiply a small number of DNA strands for analysis, a process which can introduce biases into data. Instead, the HeliScope Sequencer actually sequences the DNA strand itself, enabling direct, high-precision measurement.

In a paper published in Genome Research, RIKEN researchers confirm that this direct approach reduces biases and generates highly reproducible data from between 5 micrograms to as little as 100 nanograms of total RNA. A comparison using a leukemia cell line (THP-1) and a human cervical cancer cell line (HeLa) further shows that results from the technique are closely correlated to those from traditional microarray analysis. By making possible high-precision gene expression analysis from tiny samples, HeliScopeCAGE greatly expands the scope of research at the OSC, strengthening the institute's role in Japan as a hub for next-generation genome analysis.


'/>"/>

Contact: Alistair R. R. Forrest
public@gsc.riken.jp
81-455-039-222
RIKEN
Source:Eurekalert  

Related biology technology :

1. Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems
2. Signal Genetics and Caris Life Sciences Announce Commercialization Partnership for New Myeloma Gene Expression Test
3. Genedata Expressionist 6 with New Refiner MS Capabilities Debuts at 58th ASMS Conference on Mass Spectrometry
4. Hypoxia Induces Stem Cell Gene Expression, Protein Production and Cell Surface Markers
5. RIKEN Center for Genomics Medicine Chooses Genedata Expressionist for High Throughput Proteomics R&D
6. WaferGen to Launch Human MicroRNA Panel for Gene-Expression Profiling of 885 MicroRNA for Its Service Using the SmartChip(TM) Real-Time PCR System
7. Genedata Expressionist with New Transcriptomics Biomarker Capabilities Advances Drug Development for Personalized Medicine
8. DNA2.0 Unlocks The Secrets Of Optimized Gene Design: Company Identifies Codon Choice Design Principles for Optimal Protein Expression
9. DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches
10. Genedata Launches Expressionist with Expanded Mass Spectrometry Biomarker Discovery Capabilities
11. GenomeQuest, Inc. Announces Digital Gene Expression Solution Within Its Sequence Data Management Platform
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
HeliScopeCAGE: A new gene expression analysis technique on a single molecule sequencer
(Date:1/17/2017)... , Jan. 17, 2017 Roka Bioscience, Inc. (NASDAQ: ... advanced testing solutions for the detection of foodborne pathogens today announced ... Ms. Duseau succeeds Paul Thomas , the Company,s President and ... the Company,s Board of Directors.  The changes are effective today. ... Directors, effective today. ...
(Date:1/17/2017)... 17, 2017 Research and Markets has ... Diagnostics - Technologies, Markets and Companies" to their offering. ... ... remarkably during the past few years. More than 1,000 companies ... and 342 of these are profiled in the report along ...
(Date:1/17/2017)... , ... January 17, 2017 , ... Pono Ola ... peaceful and healthy lifestyle, announced today the official launch of its much-anticipated Pono Board: ... mind. , In development for over a year, the patented Pono Board ...
(Date:1/17/2017)... , Jan. 17, 2017  Protagonist Therapeutics, Inc. ... it has initiated a global Phase 2b induction ... peptide that targets alpha4beta7 integrin. The aim of ... to evaluate the safety/tolerability and efficacy of PTG-100 ... moderate to severe active disease. ...
Breaking Biology Technology:
(Date:1/12/2017)... 2017  Trovagene, Inc. (NASDAQ: TROV ), ... announced that it has signed agreements with seven strategic ... Middle East for commercialization of ... first wave of international distribution agreements for Trovagene,s CLIA ... The initial partners will introduce Trovagene,s liquid ...
(Date:1/6/2017)... , Jan. 6, 2017  Privately-held CalciMedica, ... studies in healthy volunteers of a novel calcium ... treat acute pancreatitis. Acute pancreatitis, ... a mild disorder, but can be very serious.  In ... and sepsis, where extended hospital stays, time in ...
(Date:1/3/2017)... 3, 2017 Onitor, provider of digital health ... Track, an innovative biometric data-driven program designed to aid ... at the 2017 Consumer Electronics Show (CES) in ... the U.S., the World Health Organization (WHO), have identified ... adults who are overweight or obese. WHO also states ...
Breaking Biology News(10 mins):