Navigation Links
Harvard's Wyss Institute team creates versatile 3d nanostructures using DNA 'bricks'
Date:12/4/2012

Researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University have created more than 100 three-dimensional (3D) nanostructures using DNA building blocks that function like Lego bricks a major advance from the two-dimensional (2D) structures the same team built a few months ago.

In effect, the advance means researchers just went from being able to build a flat wall of Legos, to building a house. The new method, featured as a cover research article in the 30 November issue of Science, is the next step toward using DNA nanotechnologies for more sophisticated applications than ever possible before, such as "smart" medical devices that target drugs selectively to disease sites, programmable imaging probes, templates for precisely arranging inorganic materials in the manufacturing of next generation computer circuits, and more.

The nanofabrication technique, called "DNA-brick self-assembly," uses short, synthetic strands of DNA that work like interlocking Lego bricks. It capitalizes on the ability to program DNA to form into predesigned shapes thanks to the underlying "recipe" of DNA base pairs: A (adenosine) only binds to T (thymine) and C (cytosine) only binds to G (guanine).

Earlier this year, the Wyss team reported in Nature how they could create a collection of 2D shapes by stacking one DNA brick (42 bases in length) upon another (http://wyss.harvard.edu/viewpressrelease/84/wyss-institute-develops-new-nanodevice-manufacturing-strategy-using-selfassembling-dna-building-blocks-).

But there's a "twist" in the new method required to build in 3D.

The trick is to start with an even smaller DNA brick (32 bases in length), which changes the orientation of every matched-up pair of bricks to a 90 degree anglegiving every two Legos a 3D shape. In this way, the team can use these bricks to build "out" in addition to "up," and eventually form 3D structures, such as a 25-nanometer solid cube containing hundreds of bricks. The cube becomes a "master" DNA "molecular canvas"; in this case, the canvas was comprised of 1000 so-called "voxels," which correspond to eight base-pairs and measure about 2.5 nanometers in size meaning this is architecture at its tiniest.

The master canvas is where the modularity comes in: by simply selecting subsets of specific DNA bricks from the large cubic structure, the team built 102 3D structures with sophisticated surface features, as well as intricate interior cavities and tunnels.

"This is a simple, versatile and robust method," says Peng Yin, Ph.D., Wyss core faculty member and senior author on the study.

Another method used to build 3D structures, called DNA origami, is tougher to use to build complex shapes, Yin said, because it relies on a long "scaffold" strand of DNA that folds to interact with hundreds of shorter "staple" strands and each new shape requires a new scaffold routing strategy and hence new staples. In contrast, the DNA brick method does not use any scaffold strand and therefore has a modular architecture; each brick can be added or removed independently.

"We are moving at lightning speed in our ability to devise ever more powerful ways to use biocompatible DNA molecules as structural building blocks for nanotechnology, which could have great value for medicine as well as non-medical applications," says Wyss Institute Founding Director Don Ingber, M.D., Ph.D.


'/>"/>
Contact: Kristen Kusek
kristen.kusek@wyss.harvard.edu
617-432-8266
Wyss Institute for Biologically Inspired Engineering at Harvard
Source:Eurekalert  

Related biology technology :

1. Harvards Wyss Institute creates living human gut-on-a-chip
2. Masaryk Memorial Cancer Institute Reduces Losses and Administrative Overhead With Ekahau RTLS
3. Van Andel Institute Partners with UNCF to Recruit Students to Biomedical Research Careers
4. The Pharmabiotic Research Institute (PRI) is Preparing to Publish its First Works on the Therapeutic and Medicinal Potential of Probiotics
5. Michael A. Marletta Takes Office as New President of Scripps Research Institute
6. HealthCare Institute of New Jersey Releases 2011 Biopharmaceutical and Medical Technology Economic Impact Data
7. QIAGEN and Max Planck Institute for Infection Biology Collaborate to Develop Assay for Active TB Risk in Individuals With Latent Infection
8. DFH Pharma to Collaborate With National Cancer Institute to Develop Second-Generation HIV Maturation Inhibitor Drugs
9. Niels Bohr Institute gets top researcher from Harvard
10. Van Andel Research Institute Study Provides New Details of Fundamental Cellular Process
11. Lupus Research Institute Awards $3.6 Million for Novel Studies Driving Wide-Ranging New Science in Lupus
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Harvard's Wyss Institute team creates versatile 3d nanostructures using DNA 'bricks'
(Date:7/17/2017)... (PRWEB) , ... July 17, ... ... of technology-enabled clinical trial solutions, today announced safety software company AB Cube ... the eHealth Solutions business segment to advance technology innovation across life sciences ...
(Date:7/17/2017)... ... July 17, 2017 , ... ... has developed and intends both to manufacture and sell reagents used for diagnostic ... Capital Market. , Headquartered in Sandy, Utah, Co-Diagnostics’ intellectual property and technologies are ...
(Date:7/15/2017)... ... July 15, 2017 , ... Cuvette manufacturer FireflySci has been ... time, the people at FFS have learned that their biggest asset was their amazing ... able to launch new products to meet the changing needs of scientists at a ...
(Date:7/14/2017)... Liege, Belgium/ Denville, USA/ Martinsried, Germany (PRWEB) , ... ... ... today announced a newly developed standardized solution for sample preparation of proteins to ... Kit) and the Diagenode Bioruptor® sonication system. , The PreOmics iST Kit ...
Breaking Biology Technology:
(Date:4/6/2017)... 2017 Forecasts by Product Type ... by End-Use (Transportation & Logistics, Government & Public Sector, ... Fossil Generation Facility, Nuclear Power), Industrial, Retail, Business Organisation ... Are you looking for a definitive report on the ... ...
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
(Date:3/28/2017)... -- The report "Video Surveillance Market by ... Devices), Software (Video Analytics, VMS), and Service (VSaaS, Installation ... 2022", published by MarketsandMarkets, the market was valued at ... reach USD 75.64 Billion by 2022, at a CAGR ... considered for the study is 2016 and the forecast ...
Breaking Biology News(10 mins):