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Global Genetic Engineering Industry
Date:4/26/2012

thers 51

 

Table 12: Gene Therapy Clinical Development by Vectors Used

(2011): Percentage Breakdown of Number of Trials for

Adenovirus, Retrovirus, Plasmid DNA, Vaccinia Virus,

Lipofection and Others 51

Gene Therapy Industry in Europe and the US - A Comparison 51

 

7. GENE AMPLIFICATION TECHNOLOGY 53

Definition of Gene Amplification 53

Target vs. Signal Amplification 53

Available Types of Gene Amplification Technologies 53

Polymerase Chain Reaction 53

The PCR comprises four major components: 53

Reagents and Equipment Required for PCR 54

Reaction Steps 54

Denaturation 54

Annealing 54

Extension 54

Detection of Amplified Product/DNA 55

(Q&Q) PCR 55

Reverse Transcriptase PCR (RT-PCR) 56

Alternative Technologies to PCR 56

OmniPlex 56

Genome-wide disease association studies 56

Pharmacogenomics studies 56

Molecular diagnostics and forensics 56

Additional OmniPlex Applications in Development 56

Strand Displacement Amplification 57

Ligase Chain Reaction 57

LCR Involves a Cyclic two-Step Reaction 57

Gene Amplification Technology (GAT) 57

Hybrid Capture 57

Isothermal DNA Amplification Techniques 58

Rolling Circle Amplification in Diagnostics 58

Ican Isothermal Gene Amplification Technology 58

BioHelix HDA Technology 58

Other Amplification Technologies 58

Signal Amplification Technology 58

Transcription Mediated Amplification 59

DNA Cleavage-based Signal Amplification 59

Ramification Amplification Method 59

End Use Market Analysis 59

Medical and Diagnostics 59

Role of Amplification Technology in the Diagnoses of

Infectious Diseases 60

Diagnosis of Infectious Diseases by PCR 60

Detection of Antimicrobial Resistance 61

PCR Based Nucleic
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