Navigation Links
Gladstone scientists identify role of tiny RNAs in controlling stem cell fate
Date:3/5/2008

SAN FRANCISCO, CA March 6, 2008--Researchers at the Gladstone Institute of Cardiovascular Disease (GICD) and the University of California, San Francisco have identified for the first time how tiny genetic factors called microRNAs may influence the differentiation of pluripotent embryonic stem (ES) cells into cardiac muscle. As reported in the journal Cell Stem Cell, scientists in the lab of GICD Director, Deepak Srivastava, MD, demonstrated that two microRNAs, miR-1 and miR-133, which have been associated with muscle development, not only encourage heart muscle formation, but also actively suppress genes that could turn the ES cells into undesired cells like neurons or bone.

Understanding how pluripotent stem cells can be used in therapy requires that we understand the myriad processes and factors that influence cell fate, said Dr. Srivastava. This work shows that microRNAs can function both in directing how ES cells change into specific cellsas well as preventing these cells from developing into unwanted cell types.

The differentiation of ES cells into heart cells or any other type of adult cell is a very complicated process involving many factors. MicroRNAS, or miRNAs, seem to act as rheostats or dimmer switches to fine-tune levels of important proteins in cells. More than 450 human miRNAs have been described and each is predicted to regulate tens if not hundreds of proteins that may determine cellular differentiation.

While many ES cell-specific miRNAs have been identified, the role of individual miRNAs in ES cell differentiation had not previously been determined. The Gladstone team showed that miRNAs can control how pluripotent stem cells determine their fate, or cell lineage in this case as cardiac muscle cells.

Specifically, they found that miR-1 and miR-133 are active at the early stages of heart cell formation, when an ES cell is first deciding to become mesoderm, one of the three basic tissue layers in mammals and other organisms. Activity of either miR-1 or miR-133 in ES cells caused genes that encourage mesoderm formation to be turned on. Equally important, they caused other genes that would have told the cell to become ectoderm or endoderm to turn off. For example, expression of a specific factor called Delta-like 1 was repressed by miR-1. Removal of this factor from cells by other methods also caused the cells to begin transforming into heart cells.

Our findings provide insight into the fine regulation of cells and genes that is needed for a heart to form, said Kathy Ivey, PhD, a California Institute of Regenerative Medicine (CIRM) postdoctoral fellow and lead author on the study. By better understanding this complicated system, in the future, we may be able to identify ways to treat or prevent childhood and adult diseases that affect the heart.


'/>"/>

Contact: Valerie Tucker
vtucker@gladstone.ucsf.edu
415-734-2019
Gladstone Institutes
Source:Eurekalert

Related biology technology :

1. Gladstone scientists uncover potential mechanism of memory loss in Alzheimers disease
2. K-State Preparing to Share Expertise With Bioscientists
3. Nanotechs health, environmental impacts worry scientists and the public
4. Transplanted cells may hold the key to curing hemophilia A, Einstein scientists report
5. UA optical scientists add new, practical dimension to holography
6. Berkeley scientists bring MRI/NMR to microreactors
7. Venter Institute Scientists Create First Synthetic Bacterial Genome
8. American Scientists Named as Laureates of the 2008 (24th) Japan Prize
9. Scientists discover new method of observing interactions in nanoscale systems
10. Carbon offset warning from international team of scientists
11. Study Examines Media Preferences of Life Scientists in Applied Markets
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2017)... ... April 20, 2017 , ... Parallel6™ , the leader in ... today that they were named one of the 2017 Top 10 eClinical Trial ... in the pharmaceutical industry. , “We take pride in honoring Parallel6 as one of ...
(Date:4/20/2017)... N.J. and PETACH TIKVAH, Israel ... BCLI), a leading developer of adult stem cell technologies for ... Executive Officer, will present at the Alliance for Regenerative Medicine,s ... Day on Thursday, April 27, 2017 at 09:40 EDT in ... Ralph Kern , MD, MHSc, Chief Medical Officer & ...
(Date:4/19/2017)... ... ... A number of new instruments have recently emerged to accommodate different applications ... and Cell Analysis Education Webinar Series , will focus on advances in the Invitrogen™ ... applications. , Many flow cytometers have unique capabilities and the Attune NxT Flow ...
(Date:4/19/2017)... ... April 19, 2017 , ... Nobilis Therapeutics Announces Completion ... to Leverage Clinical Data in its Upcoming Post Traumatic Stress Disorder Trial , ... patient clinical trial assessing efficacy of its NBTX-001, a xenon-based therapeutic in the treatment ...
Breaking Biology Technology:
(Date:4/5/2017)... 5, 2017 Today HYPR Corp. , ... server component of the HYPR platform is officially ... end-to-end security architecture that empowers biometric authentication across Fortune ... already secured over 15 million users across the financial ... connected home product suites and physical access represent a ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
(Date:3/30/2017)... 2017 Trends, opportunities and forecast in this ... technology (fingerprint, AFIS, iris recognition, facial recognition, hand geometry, ... end use industry (government and law enforcement, commercial and ... and others), and by region ( North America ... Asia Pacific , and the Rest of the ...
Breaking Biology News(10 mins):