These studies describe the first isogenic prostate cancer cell lines that metastasize reliably in immune competent mice. Previous studies were in immune deficient mice.
"This oncogene signature shows further value over current biomarkers of prediction and outcomes," said Dr. Pestell. "Such a signature and cell line may also enable the identification of targets for therapies to better treat prostate cancer, which takes the lives of over 27,000 men a year."
In breast cancer, the identification of tumor subsets with various gene signatures has improved clinical care for patients because of targeted therapies.
The work here by the KCC aims to identify gene patterns and subsequent tests in prostate cancer that could serve similar purposes. But to help develop such therapies, model systems that closely resemble human disease are required. To date, there have been several limitations with currently available cell lines.
Although important transplantation experiments have been conducted using human prostate cancer cell lines in immune deficient animals, the immune system plays an important role in prostate cancer onset and progression making it imperative to develop prostate cancer cell lines that can be studied in immune competent animals.
Also, although the transgenic mouse has been an effective model to study the molecular basis of human cancers, the prostate cancer mouse models have long latency and often unpredictable metastasis.
Here, the researchers succeeded in overcoming these issues.
The oncogene-specific prostate cancer molecular signatures were recapitulated in human prostate cancer and validated in distinct populations of patients as a prognostic and diagnostic test.
What's more, the researchers demonstrated how the isogenic prostate cancer cell lines metastasized in immune-competent mice.
"Identification of gene signatures in b
|Contact: Steve Graff|
Thomas Jefferson University