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From the mouths of monkeys: Swab technique spots tuberculosis in non-human primates
Date:6/21/2012

Tuberculosis can be a serious threat to monkeys and apes. A new technique for detecting the tuberculosis-causing bacteria could help in protecting the health of primate populations.

The method can spot TB even among infected primates that show no outward sign of disease, but are still capable of spreading infection to others of their kind.

Existing tests for TB in primates are difficult to apply and give unreliable results, often failing to detect infections.

With the new approach, researchers obtained the first published evidence of TB pathogens in the mouths of Asian monkeys living near people.

The study appears in the latest issue of the American Journal of Primatology. Dr. Lisa Jones-Engel, a senior research scientist at the National Primate Research Center at the University of Washington, headed the international project.

Her team worked in six Asian countries and the Rock of Gibraltar, places where people come into frequent contact with macaques.

The monkeys are kept as pets, or live in temples, range freely throughout urban neighborhoods, reside in nature parks or zoos, or perform as entertainers.

The researchers took cheek swabs or tossed the monkeys tasty chew sticks to obtain samples from the oral cavity, as TB usually enters and exits the body through the mouth and nose.

The scientists then analyzed the specimens to look for DNA from tuberculosis bacteria.

They applied a test designed by UW National Primate Research Center Alicia Wilbur and her colleagues, who study the origin and evolution of infectious disease.

Their test amplifies a small segment of DNA known as IS6110 that exists only in certain species of mycobacteria, the causative agents of TB.

Tuberculosis DNA was found among the Asian monkey populations living in areas with high levels of human TB, but not in the Gibraltar macaques. TB prevalence in humans is relatively low i
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Contact: Leila Gray
leilag@u.washington.edu
206-685-0381
University of Washington
Source:Eurekalert  

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