Navigation Links
Fluorescent glass SRMs are new tool for spectroscopy

Researchers at the National Institute of Standards and Technology (NIST) have developed two new calibration tools to help correct and validate the performance of analytic instruments that identify substances based on fluorescence.

Recent years have seen a significant increase in the development and use of fluorescence-based analytic techniques. Researchers can detect, measure and identify unknown substancespotentially including chemical and biological weaponsusing spectroscopic techniques. In fluorescence spectroscopy, scientists send a beam of light at a certain wavelength into a sample, exciting electrons in particular analytes or fluorescent labels, which then emit light at longer wavelengths with measurable energy levels. This resulting spectral signature, recorded by a fluorescence spectrometer, is distinct for different fluorescent compounds. Many of these assays are being used in areasincluding clinical diagnostics, environmental monitoring and drug developmentwhere regulatory requirements are strict and may require standards for instrument qualification and method validation.

To meet these needs, NIST has developed two ready-to-use, fluorescent glass Standard Reference Materials (SRMs), about the size of a pack of a gum, whose certified values can be used to correct fluorescence emission spectra for relative intensity. SRM 2940 (Orange emission) has certified values for emission wavelengths from 500 to 800 nanometers when excited with light at 412 nm; SRM 2941 (Green emission) has certified values for emission wavelengths from 450 to 650 nm when excited with light at 427 nm.

To use SRM 2941 to calibrate a fluorescence spectrometer, for instance, investigators would excite the glass with light at 427 nm and collect the resulting fluorescence emission from 450 nm to 650 nm. Spectral correction factors for the instrument then could be determined by comparing the measured intensity values to the certified values. The fluorescence spectrum of any unknown sample taken on that instrument that emits from 450 nm to 650 nm then could be corrected to yield its true spectral shape. These standards also are resistant to photodegradation, making them good performance validation standards. Researchers can use them on a day-to-day basis to validate performance by simply measuring their fluorescence intensity under the same conditions, even for fluorescence instruments with non-tunable wavelength selectors, such as filter-based fluorometers and microscopes.

Standard Reference Materials are among the most widely distributed and used products from NIST. The agency prepares, analyzes and distributes more than 1300 different materials that are used throughout the world to check the accuracy of instruments and test procedures.


Contact: Michael Baum
National Institute of Standards and Technology (NIST)

Related biology technology :

1. Automated Fluorescent-Tag Cycle Sequencing
2. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
5. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
6. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
7. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
8. Performance of fluorescent dyes with LEADseeker Multimodality Imaging System
9. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
10. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
11. Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer
Post Your Comments:
(Date:11/25/2015)... 25, 2015 2 nouvelles études permettent ... les différences entre les souches bactériennes retrouvées dans la ... des êtres humains . Ces recherches  ouvrent une nouvelle ... prise en charge efficace de l,un des problèmes ... chats .    --> 2 nouvelles études ...
(Date:11/25/2015)... (PRWEB) , ... November 25, 2015 , ... ... and the Organization of Black Aerospace Professionals (OPBAP) has been formalized with the ... other AMA team leaders met with OPBAP leaders Capt. Karl Minter and Capt. ...
(Date:11/24/2015)... ... 2015 , ... The United States Golf Association (USGA) today announced Dr. Bruce ... Presented annually since 1961, the USGA Green Section Award recognizes an individual’s distinguished service ... , Clarke, of Iselin, N.J., is an extension specialist of turfgrass pathology in the ...
(Date:11/24/2015)... ... November 24, 2015 , ... International Society ... one of the premier annual events for pharmaceutical manufacturing: 2015 Annual Meeting. The ... where ISPE hosted the largest number of attendees in more than a decade. ...
Breaking Biology Technology:
(Date:11/20/2015)... 20, 2015 NXTD ) ("NXT-ID" ... the growing mobile commerce market and creator of the ... , was recently interviewed on The RedChip Money ... this weekend on Bloomberg Europe , Bloomberg Asia, ... --> NXTD ) ("NXT-ID" or the "Company"), a ...
(Date:11/18/2015)... PHILADELPHIA , Nov. 18, 2015  As new ... in children, doctors and other healthcare providers face challenges ... counsel families and patients. In addition, as more children ... into a patient,s adulthood and old age. ... The Children,s Hospital of Philadelphia (CHOP) . ...
(Date:11/17/2015)... , November 17, 2015 ... 19 novembre  2015.  --> Paris , ... --> DERMALOG, le leader de l,innovation biométrique, a ... fois passeports et empreintes sur la même surface de ... passeports et l,autre pour les empreintes digitales. Désormais, un ...
Breaking Biology News(10 mins):