Navigation Links
Erythromycin A produced in E. coli for first time
Date:11/24/2010

MEDFORD/SOMERVILLE, Mass. -- Researchers at Tufts University School of Engineering have reported the first successful production of the antibiotic erythromycin A, and two variations, using E. coli as the production host.

The work, published in the November 24, 2010, issue of Chemistry and Biology, offers a more cost-effective way to make both erythromycin A and new drugs that will combat the growing incidence of antibiotic resistant pathogens. Equally important, the E. coli production platform offers numerous next-generation engineering opportunities for other natural products with complex biosynthetic pathways.

"We have now established E. coli as a viable option for making erythromycin A and as a platform for the directed production of erythromycin analogs. Our ability to fully manipulate erythromycin A's biosynthetic pathway to expand molecular diversity and antibiotic activity help sets a precedent for producing other similarly complex and medicinally relevant natural products," said lead researcher Blaine Pfeifer, Ph.D., assistant professor of chemical and biological engineering at Tufts.

Erythromycin A is a potent weapon in the treatment of bacterial infections. The bacterium Saccharopolyspora erythraea, which is found in the soil, naturally produces several variants of erythromycin. Erythromycin A is the most common and most biologically active.

Because of the challenges associated with engineering Saccharopolyspora erythraea, researchers have hoped to achieve the complete production of erythromycin A using E. coli. More than 20 enzymes must work in concert to create the erythromycin A molecule. This genetic and biochemical complexity makes synthesis notoriously hard. Previous research had reported manufacture of erythromycin A intermediates in E. coli but not the final product.

"To transfer and reconstitute these biosynthetic pathways is very difficult. In fact, e
'/>"/>

Contact: Kim Thurler
kim.thurler@tufts.edu
617-627-3175
Tufts University
Source:Eurekalert

Page: 1 2

Related biology technology :

1. SemBioSys eligible to proceed with Phase I/II plant-produced insulin trial after submission of IND
2. Martek to be the Sole Source Supplier of DHA and ARA for Infant Formulas Produced By Grupo Ricap
3. SemBioSys begins phase I/II trial of insulin produced in plant seeds
4. SemBioSys receives US$2.5 million from option agreement with MannKind for plant-produced insulin
5. Semiconducting nanotubes produced in quantity at Duke
6. CEL-SCI Corporation to Launch Aseptic Filling for Stem Cell Produced Therapies and Other Biological Products
7. SemBioSys Genetics Inc. Announces Clinical Results with Plant-Produced Insulin
8. Inaugural Pharma Exchange Conference Produced by Informex to Draw Together Milwaukee, Wisconsin Chemical Industry
9. China-Biotics, Inc. Announces Conference Call to Discuss First Quarter 2009 Financial Results
10. MEDRAD Installs First Intego(TM) PET Infusion System
11. Pioneering IVF Technique Produces Region's First Pregnancy
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/22/2014)... September 22, 2014 Pearl's Premium Ultra ... in drought tolerant grass seed this week with the ... The announcement coincides with the fact that it is ... as many believe. , As of the date ... favorable reviews by industry experts and the media including: ...
(Date:9/22/2014)... LEGO-like components developed by researchers at the USC ... to build a 3-D microfluidic system quickly and ... hand. , Microfluidic systems are used in ... precisely manipulate small volumes of fluids for use ... pathogen detection, clinical diagnostic testing, and synthetic chemistry. ...
(Date:9/22/2014)... have generated a new star-shaped molecule made up of interlocking ... created. , Known as a ,Star of David, ... a quarter of a century and the team,s findings are ... Consisting of two molecular triangles, entwined about each other three ... each triangle is 114 atoms in length around the ...
(Date:9/22/2014)... 2014 Lawrence Livermore National Laboratory ... on Friday renewed their institutional commitment by signing ... years to expand the collaborative work in the ... climate, energy and environmental sciences. , This renewal ... signed in December 2009 and is a framework ...
Breaking Biology Technology:Pearl's Premium Ultra Low Maintenance Lawn Seed Announces A Major Breakthrough In Drought Tolerant Grass Seed 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 3Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 4Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 5Lawrence Livermore Renews Pact with Georgetown University to Expand Research and Education in Science and Policy 2
... Solutions, Inc. today,announced the successful completion of a ... lumbar spine by Dr. Vijay Goel,s laboratory at,the ... of Facet,Solutions, ACADIA(TM) Facet Replacement System (AFRS(TM)) with ... a,polymeric disc replacement device in a finite element ...
... can be assembled into units, which can transform the ... electrical current to cooling. An effective utilization requires however ... good electrical, but low thermal conductivity. , - ... can have the desired low thermal conductivity without degrading ...
... from the U.S. Department of Energy (DOE) to commercialize cellulosic ethanol ... ... October 7, 2008-- POET, the largest producer of ethanol in the ... U.S. Department of Energy (DOE) to commercialize cellulosic ethanol production technology. ...
Cached Biology Technology:Facet Solutions Completes Facet Joint and Disc Replacement Study 2New knowledge about thermoelectric materials could give better energy efficiency 2POET Receives Additional $76 Million in Federal Funding For Commercialization of Cellulosic Ethanol 2
(Date:9/23/2014)... 2014 Newest Market IPO,s ... position for significant investor attention with news concerning shareholder updates ... today are: NXT-ID, Inc. (NASDAQ: NXTD ), Alibaba ... (NYSE: MBLY ), GoPro, Inc. (NASDAQ: ... NXT-ID, Inc. (NASDAQ: NXTD ) recently completed an ...
(Date:9/23/2014)... Most organisms, including humans, have parasitic DNA fragments called ... genetic instructions in the process. And that phenomenon can ... at the University of Rochester now report that the ... age when a multi-function protein stops keeping them in ... , In a study published today in Nature ...
(Date:9/22/2014)... September 23, 2014 A new American Cancer Society ... Assistance Program (SNAP), previously known as the food ... with income eligible non-participants. The authors say the ... at enhancing the dietary quality of SNAP participants. ... individuals and households with the resources to obtain ...
Breaking Biology News(10 mins):Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 2Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 3Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 4Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 5Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 6Latest Blockbuster IPOs and Up Listings for Global Tech Stocks Attracting Major Attention as Traders Focus on Key Economic Data Due this Week 7A multi-function protein is key to stopping genomic parasites from 'jumping' 2Federal food program puts food on the table, but dietary quality could be improved 2
... important sources of social and emotional support for "everyday people," ... published by the American Psychological Association. And, the ... people in their lives as to their animals, indicating no ... relationships with other people, or that people relied more on ...
... (STMS) business of global publisher John Wiley & Sons, ... and proportion of its journal titles with an impact ... ISI 2010 Journal Citation Reports (JCR). This ... and includes 58 titles which have received their first ...
... New research reports a high prevalence of vitamin ... Seasonal variation in vitamin D levels was not observed ... published today in Arthritis Care & Research , ... also show no association between disease activity and vitamin ...
Cached Biology News:The truth about cats and dogs: Pets are good for mental health of 'everyday people' 2Wiley-Blackwell announces continued growth in impact factor journals 2Wiley-Blackwell announces continued growth in impact factor journals 3Wiley-Blackwell announces continued growth in impact factor journals 4Wiley-Blackwell announces continued growth in impact factor journals 5Vitamin D insufficiency prevalent among psoriatic arthritis suffers 2