Navigation Links
Editing the genome
Date:7/14/2011

rrent methods by two orders of magnitude, said Harris Wang, a research fellow in Church's lab at the Wyss Institute for Biologically Inspired Engineering who shares lead-author credit on the paper with Farren Isaacs, an assistant professor of molecular, cellular and developmental biology at Yale University and former Harvard research fellow, and Peter Carr, a research scientist at the MIT Media Lab.

In the genetic code, most codons specify an amino acid, a protein building block. But a few codons tell the cell when to stop adding amino acids to a protein chain, and it was one of these "stop" codons that the Harvard researchers targeted. With just 314 occurrences, the TAG stop codon is the rarest word in the E. coli genome, making it a prime target for replacement. Using a platform called multiplex automated genome engineering, or MAGE, the team replaced instances of the TAG codon with another stop codon, TAA, in living E. coli cells. (Unveiled by the team in 2009, the MAGE process has been called an evolution machine for its ability to accelerate targeted genetic change in living cells.)

While MAGE, a small-scale engineering process, yielded cells in which TAA codons replaced some but not all TAG codons, the team constructed 32 strains that, taken together, included every possible TAA replacement. Then, using bacteria's innate ability to trade genes through a process called conjugation, the researchers induced the cells to transfer genes containing TAA codons at increasingly larger scales. The new method, called conjugative assembly genome engineering, or CAGE, resembles a playoff bracketa hierarchy that winnows 16 pairs to eight to four to two to onewith each round's winner possessing more TAA codons and fewer TAG, explains Isaacs, who invokes "March Madness."

"We're testing decades-old theories on the conservation of the genetic code," Isaacs said. "And we're showing on a genome-wide scale that we're able to make these changes."

<
'/>"/>
Contact: David Cameron
communications@hms.harvard.edu
617-432-0442
Harvard Medical School
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. New Sigma Advanced Genetic Engineering Labs Offers Research Community Unique Rodent Models Developed Using Exclusive Gene-Editing Technology
2. Sigma-Aldrich Launches Breakthrough Genome-Editing Tools
3. Quest Diagnostics and Genomic Vision Form Strategic Collaboration to Develop Genome-based Laboratory Tests
4. Phalanx Biotech Group Launches Rice Genome OneArray®
5. Further Analysis on Improved Genome Assembly Indicates the Outbreak E. coli has Complex Genetics With Resistance to at Least Eight Antibiotics
6. Cellectis bioresearch Appoints Wako as Exclusive Distributor of its Genome Customization Solutions in Japan
7. GenomeQuest to Lead Biomarker World Congress Panel on Applying Whole Genome Sequencing to Developing Companion Diagnostics
8. Selexis SA and the University of Lausanne Announce the Complete Sequencing of the Selexis SURE CHO-M Genome
9. SG Biofuels Advances Jatropha Biotech Platform with Application of Genetic Markers to Reference Genome
10. Sigma® Life Science Launches Pre-designed CompoZr® Knockout Zinc Finger Nucleases for Entire Human Genome
11. Sigma Life Science Collaborates with Cofactor Genomics to Develop Publically Available Rat Genome Database
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2015)... (PRWEB) April 20, 2015 PMG ... its 12th clinical research site: PMG Research of Christie ... operation of the clinical research department at Christie ... the Southeast to a second region within the United ... physician practice enhances PMG’s access to an additional 80,000 ...
(Date:4/20/2015)... , April 20, 2015  Ardelyx, Inc. ... focused on cardio-renal, gastrointestinal and metabolic diseases, today ... has joined the Company,s Board of Directors. Dr. ... board level experience, having built and led teams ... regulatory approval and into healthcare systems globally. ...
(Date:4/20/2015)... 20, 2015  Eli Lilly and Company (NYSE: ... targeted cancer therapies, reflecting Lilly,s diverse oncology pipeline, during ... 2015, held April 18-22 in Philadelphia ... Patients" is the theme of this year,s AACR meeting ... broadening its portfolio of therapies that accelerate the pace ...
(Date:4/20/2015)... , April 20, 2015 - ... Supramolecular Therapeutic Using the VOLVOX Platform - To be ... -  Supramolecular Assembly of Antibody-drug Conjugates Using ... - Presented on Sunday, Apr 19, 1:00 PM EST ... supramolecular therapeutic program, today announced that the company,s novel ...
Breaking Biology Technology:PMG Research Announces the Formation of its 12th Site in Champaign-Urbana, IL 2PMG Research Announces the Formation of its 12th Site in Champaign-Urbana, IL 3Ardelyx Appoints Dr. Annalisa Jenkins to Its Board of Directors 2Ardelyx Appoints Dr. Annalisa Jenkins to Its Board of Directors 3Ardelyx Appoints Dr. Annalisa Jenkins to Its Board of Directors 4Lilly Releases Early-Stage Pipeline Data at AACR Annual Meeting 2015 2Lilly Releases Early-Stage Pipeline Data at AACR Annual Meeting 2015 3Lilly Releases Early-Stage Pipeline Data at AACR Annual Meeting 2015 4Lilly Releases Early-Stage Pipeline Data at AACR Annual Meeting 2015 5Lilly Releases Early-Stage Pipeline Data at AACR Annual Meeting 2015 6Invictus Oncology Announces Two Posters to be Presented Describing its Platform Technology on Supramolecular Therapeutics at the American Association for Cancer Research Annual Meeting, 2015 2
... Sept. 15 Sigma-Aldrich Corporation (Nasdaq: SIAL ) ... Conference on Tuesday, September 21st at 12:30 PM ET in ... broadcast over the Internet available at http://investor.sigmaaldrich.com/ .  Users ... About Sigma-Aldrich: Sigma-Aldrich is a ...
... Sept. 14, 2010 -- As industries and consumers increasingly ... the Department of Energy,s Oak Ridge National Laboratory is ... A research team led by ORNL,s Nina ... new type of scanning probe microscopy called electrochemical strain ...
... SEATTLE, Sept. 14 Dendreon Corporation (Nasdaq: DNDN ... head of North American commercial operations at Genentech, a wholly-owned ... Board of Directors. "It,s been rewarding to ... periods in Dendreon,s history," said Mr. Clark. "Unfortunately, my job ...
Cached Biology Technology:ORNL scientists reveal battery behavior at the nanoscale 2Dendreon Announces Change in Board of Directors 2
(Date:4/2/2015)... Sweden , April 2, 2015 ... for Q1 2015 attributed to somewhat higher than 125 ... in the year-end report 2014 that revenues for Q1 ... that the company reported for Q4 2014. The operating ... to be negative. The complete interim report will as ...
(Date:4/1/2015)... , Apr. 1, 2015  NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... smart wallets is underway to early access pre-order customers. ... includes usage at retail outlets including Walmart, Target, AT&T, ... report Wocket was accepted at all outlets and very ...
(Date:3/31/2015)... 31, 2015  Elephant Talk Communications Corp. (NYSE MKT: ... of Software Defined Network Architecture (ET Software DNA® 2.0) ... of approximately $20.4 million for the year ended December ... for the year ended December 31, 2013. ... had completed its multi-year transition away from its legacy ...
Breaking Biology News(10 mins):Fingerprint Cards Revises Revenues for Q1 2015 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... addition to social factors when selecting a mate. In ... PLoS Genetics, scientists in China, France, and the ... be involved in mate choice in some human populations. ... Major Histocompatibility Complex (MHC), a large genomic region involved in ...
... comprehensive software package designed to significantly improve the ... been licensed by Emory University to Syntermed, an ... The software, called the Emory Reconstruction Toolbox™ ... Garcia, PhD, and Ji Chen, PhD, and their ...
... the worst that could happen after eating a slice of ... for up to a million women in the U.S., enjoying ... chronic bladder condition that causes pelvic pain. Spicy food -- ... a flare in their symptoms and intensify the pain. Researchers ...
Cached Biology News:Syntermed licenses Emory ERTb software for enhanced cardiac imaging 2Syntermed licenses Emory ERTb software for enhanced cardiac imaging 3The pepperoni pizza hypothesis 2The pepperoni pizza hypothesis 3
Arginine vasopressin receptor (AVP-V2), rat. The peptide used for immunization has no significant similarity with AVP V1a or V1b receptors....
... Immunogen: Goats were immunized ... Antibody was isolated by affinity chromatography using ... anti- human T7 affinity purified antibodies were ... method. Specificity: Antibody ...
Insect Cell Lysis Buffer 50 ml...
Ubiquitin-like protein 4A (Ubiquitin-like protein GDX). [Source:Uniprot/SWISSPROT;Acc:P11441] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Biology Products: