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EUR 1.0 Million Grant Awarded to Exosome Diagnostics and Ludwig Maximilian University from German Federal Ministry for Education and Research to Develop Biofluid-Derived Exosome Diagnostics
Date:2/1/2012

cle mRNA, is of high quality and includes both small and large RNA molecules in multiple copies.  The microvesicle/exosome population provides a stable source of RNA in blood, urine and CSF that, when isolated and prepared properly, can produce high diagnostic sensitivity for key gene mutations and gene expression levels.  

Exosome RNA biofluid diagnostic tests have potentially significant implications for disease identification, treatment and monitoring.  In many cases, tumor tissue is difficult to attain and requires either a biopsy or a surgical procedure. Repeated blood and urine measurements are minimally invasive and can provide critical information on a tumor's genetic status over time, through the course of treatment and recurrence.

"I am very excited about this project," says Dr. Carola Berking, Professor of Dermatology at Ludwig Maximilian University.  "The prospects of detecting specific cancer mutations by a fast and simple blood or urine sampling would be a major advancement and facilitate the whole procedure of personalized medicine starting from the decision to treat, which therapies to use, to the immediate monitoring of treatment effect."

To increase the capability of exosome-based technology, Exosome Diagnostics has been developing an ultra-deep, multiplexed sequencing method for use with mRNA from blood and urine exosome preparations.  The ultra-deep sequencing method allows for high sensitivity detection of rare gene mutations upregulated into exosomes by cancer cells against a background of normal or "wild-type" genes.  The massively parallel nature of next generation sequencing technology allows for hundreds of mutations to be interrogated simultaneously. Many of the mRNA targets to which exosome nucleic acid isolation and extraction technology is currently being applied are of importance in targeted molecular therapy clinical development programs.

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