A significant challenge utilizing pET vectors for bacterial expression has been the cost and uncertainty associated with vectors that have been patented. In order to tackle this problem, DNA2.0 has developed a new family of vectors called pJexpress that are free of IP constraints. pJexpress vectors are specifically engineered to express proteins at high levels and have been shown to express better than comparable pET vectors. pJexpress vectors carry a copy of the lacI gene and symmetrical lac operators to ensure optimal repression prior to induction. These vectors are available with either the T7 or T5 promoter. DNA2.0's research has shown that the T5 promoter routinely expresses proteins at higher levels comparable to T7 pET vectors and works in any E. coli host. Cloning into the pJexpress series is free of charge to DNA2.0 customers when ordering a gene.
Menlo Park, Calif. (PRWEB) March 12, 2009 -- DNA2.0, the leading gene synthesis and protein engineering company, today introduced pJexpress -- a new family of vectors for bacterial protein expression using either T7 or T5 promoters. Cloning into DNA2.0's pJexpress vectors is performed at no additional cost for gene synthesis customers and provides the freedom of working with expression vectors that are not constrained by intellectual property restrictions. The pJexpress family of vectors is available now from DNA2.0 with the same speed and PhD-level support that customers have come to expect from the world's fastest provider of synthetic genes. DNA2.0 will continue to develop new vectors with additional features in order to provide customers with the most advanced vector technology available.
"DNA2.0's pJexpress vectors routinely express protein more efficiently than traditional pET vectors and without IP constraints asso
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