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Cytogenetics - Technologies, Markets and Companies

n expression at the single molecule level 61

Bioinformatic and single molecule detection 62

6. Role of Nanobiotechnology in Cytogenetics 63

Introduction 63

Nanobiology and the cell 63

Visualization on nanoscale 64

Application of AFM for biomolecular imaging 64

Future insights into biomolecular processes by AFM 64

Use of AFM for microdissection of chromosomes 65

Scanning probe microscopy 65

Near-field scanning optical microscopy 65

Multiple single-molecule fluorescence microscopy 66

Nanoscale scanning electron microscopy 66

Nanotechnology-based FISH 66

Study of chromosomes by atomic force microscopy 66

Quantum dot FISH 66

Nanobiotechnology for single molecule detection 67

Nanolaser spectroscopy for detection of cancer in single cells 68

Carbon nanotube transistors for genetic screening 68

Quantum-dots-FRET nanosensors for single molecule detection 69

3D single-molecular imaging by nanotechnology 69

Manipulation of DNA sequence by use of nanoparticles 69

Nanofluidic/nanoarray devices to detect a single molecule of DNA 69

Nanopore technology 70

Portable nanocantilever system for diagnosis 70

Nanobiosensors 71

7. Biomarkers and Cytogenetics 73

Introduction 73

Definitions 73

Biomarkers and cytogenetics 73

Cancer biomarkers 73

Technologies for detection of cancer biomarkers 74

Telomerase as a biomarker of cancer 74

Digital karyotyping for cancer biomarkers 74

Optical systems for in vivo molecular imaging of cancer 75

Circulating cancer cells in blood as biomarkers of cancer 75

Array CGH for biomarker discovery in cancer 76

Genetic biomarkers 76

8. Applications of Cytogenetics 77

Introduction 77

Applications of cytogenetics in research 77

Cytogenetics of embryonic stem cells 77


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