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Cytogenetics - Technologies, Markets and Companies
Date:1/3/2012

H probes 39

peT-FISH™ 39

In vivo FISH 40

Applications of FISH 40

Companies involved in FISH diagnostics 41

4. Genomic Technologies relevant to Cytogenetics 43

Introduction 43

Microarrays/biochips for cytogenetics 43

Tissue microarrays 43

Chromosome copy number analysis 43

Combination of FISH and gene chips 44

Combination of CGH+SNP microarrays 44

SignatureChip® 44

Molecular Combing 45

High density oligonucleotide arrays 45

Next Generation Screening® 46

Comparative genomic hybridization 46

Array-based comparative genomic hybridization 48

aCGH vs karyotyping 48

Comparison of array CGH and multipoint FISH 49

Combined use of tissue microarrays and aCGH 49

Single-cell array CGH 49

Regulatory requirements for array CGH 50

Future prospects of aCGH 50

Whole genome expression microarrays 51

Life Technologies Expression Array System 51

Arrayit's® H25K 52

Optical Mapping 52

Single cell cytogenetics 53

Single cell PCR 53

LATE-PCR 53

AmpliGrid-System 53

Digital Counting 53

Analysis of single-cell gene expression 54

Application of single cell cytogenetics in preimplantation genetic testing 54

Direct molecular analysis without amplification 55

5. Molecular Imaging & Single Molecular Detection 57

Molecular imaging 57

Companies involved in molecular imaging 57

Single molecule detection 58

Spectrally resolved fluorescence lifetime imaging microscopy 58

Single-molecule fluorescence resonance energy transfer 59

Confocal laser scanning 59

Single Molecule Array 59

PCR systems for single molecule detection 60

Real-time PCR 60

Digital PCR 60

Emulsion PCR 61

Rolling circle amplification technology 61

Microfluidic assay for protei
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