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Cytogenetics - Technologies, Markets and Companies
Date:1/3/2012

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RNA 16

DNA transcription 16

Chromosomes 16

Mitochondrial DNA 17

Genes 17

The genetic code 17

Gene expression 18

The human genome 18

Variations in the human genome 19

Variations in DNA sequences 19

Single nucleotide polymorphisms 19

Copy number variations in the human genome 19

Genotype and haplotypes 21

Complex chromosomal rearrangements 21

Insertions and deletions in the human genome 21

Large scale variation in human genome 22

Structural variations in the human genome 22

Replication of the DNA helix 23

Transposons 23

Mapping and sequencing of structural variation from human genomes 24

2. Technologies used for cytogenetics 25

Introduction 25

Quantitative fluorescent polymerase chain reaction 25

RNA interference and cytogenetics 26

RNA-induced transcriptional silencing complex 26

Single cell genetics by siRNA ablation 26

RNAi and cancer cytogenetics 27

Role of miRNAs in cancer cytogenetics 27

Preimplantation genetic diagnosis 27

Preimplantation genetic haplotyping 28

Bioinformatics and cytogenetics 28

FISH probe design software 28

LS-CAP algorithm 29

Distance-based clustering of CGH data 29

3. Fluorescent In Situ Hybridization 31

Introduction 31

Innovative FISH technologies 33

Direct visual in situ hybridization 33

Direct labeled Satellite FISH probes 33

Chromogenic in situ hybridization (CISH) 33

Primed in situ labeling 34

Interphase FISH 34

FISH with telomere-specific probes 35

High-throughput quantitative FISH 35

Multicolor FISH 36

Multicolor chromosome banding 36

Fiber FISH 36

Use of peptide nucleic acid with FISH 36

RNA-FISH 38

Use of locked nucleic acids with FISH 38

Automation of FISH 38

Single copy FIS
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