BOGOTA, Colombia, Sept. 3, 2012 /PRNewswire/ -- Water buffalo breeding, an industry that actually uses artificial insemination, started using a new variant of the procedure in which the sex of the offspring can be chosen and it is called "Sexed Semen."
This experience had never been used for the water buffalo species until this year, when Colbufalos, a company dedicated to buffalo breeding, located in Cordoba-Colombia, and led by Juan Gonzalo Angel, obtained the first successful results.
Sexed semen became a reality in 1989, when Dr. Larry Johnson and his colleagues used a flow cytometer to sort spermatozoa and detect some differences in their DNA. The X-bearing sperm are sorted off in one direction, the Y- bearing sperm in another, and anything of undetermined sex passes straight through as waste. Since that time, this method that bio-technically separates the sperm has been applied to many farming species. And in water buffalo, this technique was investigated by Giorgio Antonio Presicce and his collaborators in 2005.
Colbufalos started their program of artificial insemination (AI) with sexed semen in late 2010, using semen from the Mediterranean race. Although no special considerations were taken to the use the sexed semen straws in the program at the farm, the water buffalo females were sorted by their better genetic characteristics and high production. The first offspring from sexed semen was obtained on December 17th, 2011. Results at first were less than average, having instances of pregnancy less than 30%. In seeking possible causes, great variability in seminal material quality, mobility and concentration was found. For the second attempt, Colbufalos decided to use a different sperm supplier named "Sexing Technologies," and necessary adjustments were made to minimize the variation in the
|SOURCE Colbufalo SAS|
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