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Clontech Laboratories, Inc. Releases the SMARTer™ Ultra Low RNA Kit for Illumina® Sequencing
Date:3/31/2011

expression in individual cells that was not previously possible.  The ability to assay the expression of individual neurons from brain slices, which we have done, allows one to correlate changes in the electrophysiology of each cell to changes in gene expression."

The SMARTer Ultra Low RNA Kit for Illumina Sequencing has been designed and validated to prepare cDNA samples for sequencing on all of Illumina's sequencing instruments, including HiSeq™ 2000. This kit greatly reduces handling of the RNA sample, thereby minimizing the risk of sample loss and preserving the original message. The efficient incorporation of known sequences at both ends of the cDNA during first strand synthesis enables researchers to perform the entire protocol in a single tube, without a separate adaptor ligation step. Moreover, SMART's high efficiency and sensitivity permits the use of very limited quantities of starting material—as little as 100 pg of total RNA—from a variety of sources. The combination of SMART technology's ability to handle very small quantities of RNA with Illumina's unique combination of long and short reads, single and paired-end sequencing, and capacity for tens of millions to billions of reads per run allows you to annotate coding SNPs, discover transcript isoforms, characterize splice junctions, and determine the relative abundance of transcripts from even the smallest samples.

About Clontech Laboratories, Inc.

Clontech Laboratories, Inc., a wholly-owned subsidiary of Takara Bio Inc., develops, manufactures, and distributes a wide range of life science research reagents under the Clontech and Takara brands. Key products include high-performance qPCR and PCR reagents (including the Ex Taq™, LA Taq™, Titanium®, and Advantage® enzymes); RT enzymes and SMART and SMARTer library construction kits; the innovative In-Fusion® cloning system; Tet-based inducible gene expression syste
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