Navigation Links
Chemists develop innovative nano-sensors for multiple proteins
Date:8/1/2013

Chemists at Johannes Gutenberg University Mainz (JGU) have developed a new method for parallel protein analysis that is, in principle, capable of identifying hundreds or even thousands of different proteins. It could be used to detect the presence of viruses and identify their type in tiny samples. At the same time, it is very cost-effective and quick. "We see possible applications of this technique in medicine, where it could be used, for example, for the rapid diagnosis of a wide range of diseases. It would be almost as easy to use as a pregnancy test strip," said Professor Carsten Snnichsen of the Institute of Physical Chemistry. The test involves placing a tiny drop of blood, saliva, or other bodily fluid on a small test strip, which is then placed in a device developed at the JGU Institute of Physical Chemistry. This device is able to identify the specific proteins in the fluid and thus allows to quickly and reliably differentiate between harmless microorganisms and dangerous pathogens.

In order to detect the many different substances present in a small sample, the sensors need to be as tiny as possible, preferably the size of nano-particles. Snnichsen's team of scientists have designed a sensor no larger than the head of a pin but capable of performing a hundred different individual tests on a surface that is only of one-tenth of a square millimeter in area. The 'test strips' consist of glass capillary tubes that have gold nano-particles as sensor elements on their internal surfaces. "We first prepare our nano-particles using short DNA strands, each of which binds to a specific type of protein," explained Janak Prasad, who developed the functionalization method. When a protein docks with one of these special DNA strands, called aptamers, the corresponding nano-particle changes its color. The color changes can be detected with the aid of a spectrometer. For this purpose, the capillary tubes are placed under a microscope designed, constructed, and provided with the necessary software by the Mainz-based team of chemists.

"We demonstrate a new approach for a multiplexed assay that detects multiple proteins simultaneously by letting a fluid flow past the randomly positioned gold nano-rods," explained Christina Rosman, first author of the study. The team from JGU's Institute of Physical Chemistry used four different target proteins to demonstrate the viability of the new concept, its ability to detect concentrations in the nanomolar range, and the possibility to recycle the sensors for more than one analysis. "We see the potential to extend our method to the simultaneous detection of hundreds or even thousands of different target substances," assert the authors in their article published in the June 2013 issue of the journal Nano Letters. Low-cost serial production of the sensors is feasible if advanced nano-fabrication methods such as nano-printing or optical trapping are used.

There are manifold possible applications of a test for multiple targets in a single procedure. The low-cost sensors could be directly used by physicians in their practices in order to detect and discriminate various types of flu viruses with which their patients could be infected. In addition, the technique would also be suitable for detecting the presence of toxins in the environment or in food, particularly in liquids such as milk or baby food, or the presence of doping or other illicit drugs.


'/>"/>

Contact: Dr. Carsten Sönnichsen
carsten.soennichsen@uni-mainz.de
49-613-139-24313
Johannes Gutenberg Universitaet Mainz
Source:Eurekalert

Related biology technology :

1. University of Utah chemists use nanopores to detect DNA damage
2. Chemists become molecular sculptors, synthesizing tiny, molecular traps
3. Biotech Industry Veteran Joseph Bolen Joins Moderna Therapeutics to be President of Research and Development
4. AACC Pilot Studies Begin to Measure What Is Normal in Childrens Development
5. GlycoVaxyn AG secures CHF 5.1 million Strategic Translation Award from the Wellcome Trust to finance clinical development of Shigella vaccine
6. Anti-Infective Services for Drug Discovery and Development, New Life Science Webinar Hosted by Xtalks
7. PolyTherics and Antitope Combine to Create Leading Provider of Solutions to Enable the Development of Better Biopharmaceuticals
8. Global Ubiquitin Proteasome Research and Development Market to Surpass $5.5 Billion by 2018: BCC Research
9. Surveyed U.S. Payers Are Significantly More Likely To Reimburse Biosimilars Developed By Multinational Manufacturers Compared With Those Developed By Regional Emerging Market Players
10. Life Technologies to Collaborate with Merck Serono for Companion Diagnostics Development and Commercialization
11. Signal-Health Develops Easy Checklist to Identify Possible Sweating Issues in Horses
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/24/2017)... ... July 24, 2017 , ... Each year, Inavero’s Best ... proven their superior service quality as rated by hiring professionals and job candidates. ... based on service quality ratings from their placed talent. , Fewer than 2% ...
(Date:7/20/2017)... ... July 20, 2017 , ... Resoundant, Inc. is ... imaging centers around the U.S. that offer MR Elastography for liver fibrosis staging. ... needle biopsy for staging liver fibrosis assessment. , “MRE:connect was created in response ...
(Date:7/20/2017)... ... July 20, 2017 , ... VIC Technology Venture ... the company’s board of directors. This addition continues to strengthen and diversify VIC’s ... Goforth, CEO and Chairman. “He is a highly accomplished business executive with a broad ...
(Date:7/20/2017)... , ... July 20, 2017 , ... Corporate Directors Forum ... its 27th annual Director of the Year Awards. , The awards will be presented ... This annual event celebrates directors who have made significantly positive contributions in the ...
Breaking Biology Technology:
(Date:3/30/2017)... KONG , March 30, 2017 The ... a system for three-dimensional (3D) fingerprint identification by adopting ground breaking ... into a new realm of speed and accuracy for use in ... at an affordable cost. ... ...
(Date:3/27/2017)... CENTRE, N.Y. , March 27, 2017 /PRNewswire-USNewswire/ ... Healthcare Information and Management Systems Society (HIMSS) Analytics ... Outpatient EMR Adoption Model sm . In addition, ... 12% of U.S. hospitals using an electronic medical ... CHS for its high level of EMR usage ...
(Date:3/23/2017)... -- Research and Markets has announced the addition of ... Industry Forecast to 2025" report to their offering. ... The Global Vehicle Anti-Theft ... 8.8% over the next decade to reach approximately $14.21 billion by ... and forecasts for all the given segments on global as well ...
Breaking Biology News(10 mins):