MADISON, Wis., March 4, 2011 /PRNewswire/ -- Cellular Dynamics International (CDI), the world's largest producer of human tissue cells for drug discovery and safety testing, today announced the publication of research demonstrating an efficient and scalable method of creating DNA integration-free, or footprint-free, induced pluripotent stem cells (iPSCs) under a feeder-free condition using chemically defined media. This method, published on March 1 in PLoS ONE, will likely be valuable for the production of clinical-grade human iPSCs.
Junying Yu, Senior Director, Advanced Development Programs, and lead author of the paper, and her colleagues at CDI improved efficiency over her previously published method that resulted in exogenous DNA-free iPSCs by employing a cocktail containing four small molecules and cultured in chemically defined media free of feeder cells. The addition of small molecules significantly improved episomal reprogramming efficiency. Elimination of feeder cells and the use of chemically defined media for both the derivation and culture of footprint-free iPSCs bring the reprogramming technology one step closer to clinical applications. In addition, the feeder cells represent a source of variability. Eliminating feeder cells makes the process more amenable to industrialization. The method was successfully applied and optimized for several cell types including human skin fibroblasts, adipose tissue-derived cells and cord blood cells.
"This publication underscores CDI's commitment to industrialized human biology," said Dr. Yu. "This method enables efficient, safe, large scale derivation of iPSCs for research applications and sets the stage for potential clinical use in the future."
"I wish to congratulate Dr. Yu on her most recent p
|SOURCE Cellular Dynamics International, Inc.|
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