Navigation Links
Carnegie Mellon scientists create rainbow of fluorescent probes
Date:3/25/2010

SAN FRANCISCOScientists at Carnegie Mellon University's Department of Chemistry and Molecular Biosensor and Imaging Center (MBIC) are advancing the state-of-the-art in live cell fluorescent imaging by developing a new class of fluorescent probes that span the spectrum from violet to the near-infrared. The new technology, called fluoromodules, can be used to monitor biological activities of individual proteins in living cells in real time. At the 239th national meeting of the American Chemical Society, Carnegie Mellon chemists and MBIC scientists will discuss recent advances in their fluoromodule technology that have produced diverse and photostable probes.

Fluoromodules, which consist of dye-protein complexes, provide alternatives to common fluorescent proteins, such as Green Fluorescent Protein (GFP), but with a wider selection of colors and the potential for significantly greater photostability, which allows scientists to image the dye for longer periods of time. This is made possible by the fact that the dye is noncovalently bound to the protein, which allows fresh dye to replace bleached dye.

"We initially isolated and characterized fluoromodules that generate fluorescence from the fluorogenic dyes thiazole orange and malachite green. We are now expanding our repertoire by synthesizing new dyes that emit in the orange and violet regions of the spectrum, and engineering proteins that bind to the new dyes with great affinity," said Chemistry Professor Bruce Armitage, co-director of the Center for Nucleic Acid Science and Technology at Carnegie Mellon and a member of the MBIC team developing the fluoromodules.

Fluoromodules are made of a fluorogen-activating protein (FAP) and a non-fluorescent dye called a fluorogen. The FAP, which is genetically expressed in a cell and tagged to a protein of interest, does not become fluorescent until it binds with its fluorogen. With the novel FAPs and associated fluorogens created by the MBIC tea
'/>"/>

Contact: Jocelyn Duffy
jhduffy@andrew.cmu.edu
412-600-0029
Carnegie Mellon University
Source:Eurekalert

Page: 1 2

Related biology technology :

1. 2010 Carnegie Science Awardees Announced
2. Carnegie Mellon MRI technology that noninvasively locates, quantifies specific cells in the body
3. MIT scientists transform polyethylene into a heat-conducting material
4. Scientists discover how ocean bacterium turns carbon into fuel
5. Scientists glimpse nanobubbles on super nonstick surfaces
6. Princeton scientists find an equation for materials innovation
7. Scientists glimpse nanobubbles on super non-stick surfaces
8. Scientists transplant nose of mosquito, advance fight against malaria
9. Penn material scientists turn light into electrical current using a golden nanoscale system
10. Seeing the quantum in chemistry: JILA scientists control chemical reactions of ultracold molecules
11. NFCR Scientists Discover Brain Tumor's “Escape Path”
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/28/2015)... 28, 2015 RXi Pharmaceuticals Corporation (NASDAQ: ... developing innovative therapeutics, primarily in the areas of dermatology ... offering of 26 million units at a price to ... of approximately $10.4 million. Each unit consists of (i) ... purchase right to purchase 0.50 of a share of ...
(Date:5/28/2015)... , May 28, 2015  GenVec, Inc. (Nasdaq: ... Douglas J. Swirsky , will present a company ... 11 a.m. EDT on Thursday, June 4, 2015. ... Swirsky,s presentation will be available at the following link:  ... on GenVec,s website.  To access, visit www.genvec.com , ...
(Date:5/28/2015)... The Fertility Centers of New England is proud ... taken on a new and expanded role as Medical ... leadership, oversight and quality assurance for the practice. , ... team of experienced reproductive endocrinologists,” said Fertility Centers of ... “Her expertise and compassionate, individualized care have helped set ...
(Date:5/28/2015)... Whitehouse Laboratories is excited to announce ... partnership with PTI Inspection Systems. Whitehouse Labs and PTI ... custom-tailored method development and validation programs using the ... agreement includes a more formal program that facilitates cross ... This enables a more effective team approach, especially as ...
Breaking Biology Technology:RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3GenVec To Present At The Jefferies Healthcare Conference 2Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2Whitehouse Labs Renews & Enhances Partnership with PTI Inspection Systems 2Whitehouse Labs Renews & Enhances Partnership with PTI Inspection Systems 3
... Cellumen, Inc., the Cellular,Systems Biology Company, announced today ... Center for Toxicology Research (NCTR), a,research center of ... Cellumen will use its proprietary CellCiphr(R) toxicity risk,assessment ... toxicity,compounds including both failed and marketed drugs for ...
... ... -, ... stage biotechnology company committed to the,research and product discovery against life-threatening and ... Therapeutics has been led since its,inception in 2006 by James Sapirstein, RPh, ...
... SGX Pharmaceuticals,(Nasdaq: SGXP ) today announced that ... to the U.S. Food and Drug Administration for ... molecule for the treatment of relapsed and refractory ... for CML is Gleevec(R). Although Gleevec is a,highly ...
Cached Biology Technology:Cellumen to Collaborate With the NCTR of the FDA to Apply Cellular Systems Biology (CSB(TM)) to Predictive Toxicology 2Tobira Therapeutics Inc. Announces Senior Management Team 2Tobira Therapeutics Inc. Announces Senior Management Team 3SGX Pharmaceuticals Submits Investigational New Drug Application for SGX393 2SGX Pharmaceuticals Submits Investigational New Drug Application for SGX393 3
(Date:5/21/2015)... May 21, 2015 According ... Market by Solutions (Hardware, Software, Services), by Applications (Surveillance ... others), by End-Users (Military & Defense & Commercial) ... Thermal Imaging Market is expected to grow from ... 2020, at a Compound Annual Growth Rate (CAGR) ...
(Date:5/19/2015)... YORK , May 19, 2015  Technology is ... both internally and in the cloud. Passwords and their ... use of OTP and standards-based specifications such as those ... the outmoded use of passwords presents for BYOD, COPE, ... a unified biometric identity protocol. In ...
(Date:5/11/2015)... , May 11, 2015  Synaptics Incorporated ... human interface solutions, today announced the appointment of ... and Chief Financial Officer, reporting to Rick ... Synaptics, current Chief Financial Officer, Kathleen Bayless ... Mr. Ali brings extensive financial ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2
... rising from deep within the Earth are fueling the ... the rim of the 19,850-foot-high Socompa volcano in the ... team. The new study shows the emission ... vents near the summit of Socompa sustains complex microbial ...
... for example food, can be kept for a long ... University of Gothenburg, Sweden, is close to providing answers ... to carry out various biological functions. The protein,s level ... these functions is dependent on the amount of water ...
... March 3, 2009 -- Ben-Gurion University of the Negev ... technology, featuring two reversible panes that will save energy ... , "The ,Seasons Window, features the only glazing system ... or high radiant temperature near a window and reduces ...
Cached Biology News:Earth's highest known microbial systems fueled by volcanic gases 2Earth's highest known microbial systems fueled by volcanic gases 3Ben-Gurion University of the Negev researchers develop new reversible, green window technology 2
...
... the autofluorescent pigment lipofuscin accumulates in ... including neurons. The presence of lipofuscin ... fluorescence microscopy in the central nervous ... and emission spectra, which overlaps with ...
Purified anti-LAT...
Polyclonal Antibody to AHI1 / Jouberin...
Biology Products: