SAN DIEGO, March 12 /PRNewswire/ -- Biomatrica, the leader in room temperature biological sample stabilization, announced the introduction of PCRboost, a reagent that enhances PCR and RT-PCR performance 5-fold or more by improving sensitivity and specificity during amplification of genomic DNA or RNA templates.
PCRboost makes it easy for scientists to amplify templates that are traditionally difficult to amplify and present in limited quantities. At the same time the number of cycles and amount of polymerase used are reduced, resulting in significant time and cost savings.
"With PCRboost, I obtained at least a 5-fold increase in the amplification of formalin-fixed paraffin embedded (FFPE) cervical cancer tissue samples compared to using water in a standard master mix," said Mina Kalantari, Ph.D., University of California, Irvine. "I start out with very small amounts of degraded DNA and then perform bi-sulfite modification for DNA methylation analysis, which leads to further degradation. Using PCRboost, I am now able to see bands that were invisible without it."
PCRboost greatly improves the amplification of difficult samples, enhancing the yield, specificity and consistency of the final product. There are no changes needed to existing PCR and RT-PCR protocols -- researchers simply replace the water in the reaction with PCRboost. Amplification is drastically improved for all samples including trace samples (less than or equal to 1 ng), degraded samples, low copy transcripts, multiplexing (i.e., STR analysis) and samples containing inhibitory factors.
"PCRboost is very easy to use -- you just add the reagent instead of water to your reaction mixture. I love the outcome!" states Kalantari.
PCRboost is currently available in three formats: a Trial Kit,
containing 150 ul of PCRboost; a 1 ml tube; and a 10 ml bottle. All three
sizes are available for purchase at http://www.biomatri
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