Navigation Links
Biomatrica's PCRboost(TM) Enables 5-Fold Improvement in Amplification of Difficult Samples
Date:3/12/2008

SAN DIEGO, March 12 /PRNewswire/ -- Biomatrica, the leader in room temperature biological sample stabilization, announced the introduction of PCRboost, a reagent that enhances PCR and RT-PCR performance 5-fold or more by improving sensitivity and specificity during amplification of genomic DNA or RNA templates.

PCRboost makes it easy for scientists to amplify templates that are traditionally difficult to amplify and present in limited quantities. At the same time the number of cycles and amount of polymerase used are reduced, resulting in significant time and cost savings.

"With PCRboost, I obtained at least a 5-fold increase in the amplification of formalin-fixed paraffin embedded (FFPE) cervical cancer tissue samples compared to using water in a standard master mix," said Mina Kalantari, Ph.D., University of California, Irvine. "I start out with very small amounts of degraded DNA and then perform bi-sulfite modification for DNA methylation analysis, which leads to further degradation. Using PCRboost, I am now able to see bands that were invisible without it."

PCRboost greatly improves the amplification of difficult samples, enhancing the yield, specificity and consistency of the final product. There are no changes needed to existing PCR and RT-PCR protocols -- researchers simply replace the water in the reaction with PCRboost. Amplification is drastically improved for all samples including trace samples (less than or equal to 1 ng), degraded samples, low copy transcripts, multiplexing (i.e., STR analysis) and samples containing inhibitory factors.

"PCRboost is very easy to use -- you just add the reagent instead of water to your reaction mixture. I love the outcome!" states Kalantari.

PCRboost is currently available in three formats: a Trial Kit, containing 150 ul of PCRboost; a 1 ml tube; and a 10 ml bottle. All three sizes are available for purchase at http://www.biomatrica.com.

About Biomatrica(R), Inc.

Biomatrica (http://www.biomatrica.com) is a San Diego-based biostability company that provides innovative technologies for stabilizing, processing, storing and shipping biological samples at room temperature. The core technology is designed for use in preserving complex biological samples and assays and is based on the principles of anhydrobiosis ("life without water"), a natural mechanism that allows multicellular organisms to survive extreme environments. Biomatrica's current products stabilize DNA and RNA with no sample degradation, thus labs can reduce their reliance on freezers and drastically reduce shipping costs. Biomatrica products are used in laboratories performing life science research, from pharmaceutical and biotechnology companies to academic research and forensics laboratories. Custom services to stabilize additional sample types are also available. Biomatrica also offers SampleWare(R) software, an easy-to-use, customizable laboratory management database that provides scientists with the means to store and organize their sample data, and directly supports samples stabilized by Biomatrica technology.


'/>"/>
SOURCE Biomatrica
Copyright©2008 PR Newswire.
All rights reserved

Related biology technology :

1. Varian Medical Systems Receives FDA 510(k) Clearance for High-Definition Collimator That Enables Ultra-Fine Intensity-Modulated Radiosurgery
2. MEDEA+ Noblanc award highlights improvements in microelectronics
3. China Biologic Products Gains Financial Support from Shandong Provincial Government on the Improvement of Production Facilities
4. Synthetech Earns National Recognition for Performance Improvement Program
5. InVitrias Animal Free Lacromin(TM) Provides a Cost-Effective Improvement Over Transferrin
6. Oncologists Want to See Significant Improvement in Overall Survival Before Adopting Targeted Polytherapy in a First-Line Setting
7. Difficult to Treat Erectile Dysfunction Patients
8. Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types
9. IBM and Imago find a crucial difficulty in semiconductor device scaling
10. McGill University Purchases GenVaults Personal Archive System to Manage Rare Cancer Samples
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/10/2016)... ... February 10, 2016 , ... ... announced that it has joined the Human Vaccines Project, a public-private partnership ... and cancer. , The Human Vaccines Project brings together leading pharmaceutical ...
(Date:2/10/2016)... ... 10, 2016 , ... HOLLOWAY AMERICA, a leading custom stainless ... Mountain Chapter 21st Annual Vendor Exhibition on Thursday, February 18, 2016. The Rocky ... its annual event, which will run from 3:00 p.m. - 8:30 p.m. at ...
(Date:2/10/2016)... ... February 10, 2016 , ... ... a new agreement with Singapore-based Global Stem Cells Network (GSCN) and its affiliate ... and Singapore in the latest adipose and bone marrow therapies. , Through ...
(Date:2/9/2016)... , ... February 08, 2016 , ... ... , Location: Baruch S. Blumberg Institute at the Pennsylvania Biotechnology Center of Bucks ... Institute and The Commonwealth Medical College (TCMC) will hold an open house for ...
Breaking Biology Technology:
(Date:1/20/2016)... 20, 2016 A market that just keeps ... from the explosion in genomics knowledge. Learn all about ... A range of dynamic trends are pushing market growth ... medicine - pharmacogenomics - pathogen evolution - next generation ... - greater understanding of the role of genetic material ...
(Date:1/18/2016)... , Jan. 18, 2016  Extenua Inc., ... that simplifies the use and access of ubiquitous ... go-to-market partnership with American Cyber.  ... extensive experience leading transformational C4ISR and Cyber initiatives ... integrating the latest proven technology solutions," said ...
(Date:1/13/2016)... 2016 http://www.researchandmarkets.com/research/7h6hnn/india_biometrics ... the  "India Biometrics Authentication & Identification ...  report to their offering.  ... the addition of the  "India Biometrics ... & Forecast (2015-2020)"  report to ...
Breaking Biology News(10 mins):