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Biomarkers - Technologies, Markets and Companies

Nucleoprotein nanodevices for detection of cancer biomarkers 80

Future prospects of application of nanobiotechnology for biomarkers 80

Bioinformatics 81

Biomarker Workflow Guide 81

Analysis of microarray data for selecting useful biomarkers 81

Role of bioinformatics in discovery of protein biomarkers 82

Role of bioinformatics in detection of cancer biomarkers 82

Biomarker databases 83

Gene networks as biomarkers 83

Role of bioinformatics in integrating various data and biomarker discovery 83

Evaluation of biomarker studies 84

3. Biomarkers and Molecular Diagnostics 85

Introduction 85

Molecular diagnostic technologies 85

Polymerase chain reaction 85

Amplification 85

Target selection 86

Detection of amplified DNA 86

Limitations of PCR 86

Real-time PCR systems 87

Limitations of real-time PCR 87

Future applications of real-time qPCR 88

Real-time qPCR for quantification of circulating mtDNA 88

Combined PCR-ELISA 88

Non-PCR methods 89

Linked Linear Amplification 89

Transcription mediated amplification 89

Rapid analysis of gene expression 89

WAVE nucleic acid fragment analysis system 90

DNA probes with conjugated minor groove binder 90

Rolling circle amplification technology 91

Gene-based diagnostics through RCAT 91

RCAT-immunodiagnostics 92

RCAT-biochips 92

RCAT-pharmacogenomics 92

Circle-to-circle amplification 92

Biochips and microarrays 93

Applications of biochips/microarrays 93

Role of biochip/microarrays in discovery of biomarkers 94

Biomarkers and high throughput molecular screening 94

Detection and expression profiling of miRNA 95

Real-time PCR for expression profiling of miRNAs 95

Real-time PCR for expression profiling of miRNAs 95

Use of LNA to explore miRNA 95

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