Navigation Links
Biologists discover an extra layer of protection for bacterial spores
Date:5/6/2010

Bacterial spores, the most resistant organisms on earth, carry an extra coating of protection previously undetected, a team of microbiologists reports in the latest issue of the journal Current Biology. Their findings offer additional insight into why spores of the bacteria that cause botulism, tetanus, and anthrax survive methods to eradicate them.

The study was conducted by researchers at New York University's Center for Genomics and Systems Biology, Loyola (Ill.) University's Medical Center, and Princeton University's Department of Molecular Biology.

The researchers studied the spores of a non-pathogenic bacterium, Bacillus subtilis, which is commonly found in soil. Although non-pathogenic, B. subtilis spores exhibit many of the same structural features of the spore-forming pathogens. In this study, the scientists examined the proteins that comprise spores' protective layers. Previous research has shown that 70 different proteins make up these layers. Less understood is how these proteins interact to form the spores' protective coats.

To do this, the researchers examined coat formation of both normal and mutant spores. In the latter case, they removed genes for selected coat proteins, allowing them to determine which proteins were necessary inand extraneous tothe formation of the spores' coats.

To observe proteins' behavior in living cells, the researchers fused the genes encoding the spores' coat proteins to a marker, a Green Fluorescent Protein (GFP). This procedure allowed them to monitor how the proteins localized to form spores' protective coats. A combination of fluorescence microscopy experiments and high-resolution image analysis enabled the researchers to overcome a theoretical limitation of light microscopy, pinpoint the location of the spores' coat proteins with a high degree of precision, and build a map of the spore coat. These experiments suggested the existence of a new outermost layer of the spore coat. They were then able to confirm the existence of this new layer using electron microscopy.

The researchers named this coat layer, located on the spores' outer surface, the "spore crust." While it has not yet been confirmed, it is possible that the spore crust is a common feature of all spore-forming bacteria, such as the botulism, tetanus, and anthrax pathogens.


'/>"/>

Contact: James Devitt
james.devitt@nyu.edu
212-998-6808
New York University
Source:Eurekalert

Related biology technology :

1. Bringing Statistics to Biologists - New Genedata Analyst Launches at Society of Toxicology 49th Annual Meeting
2. Vermillion and Collaborators Discover Protein Biomarker Associated with Peripheral Artery Disease
3. PDL BioPharma Announces Significant Strategic and Portfolio Changes to Focus on Antibody Discovery and Development
4. Novocell Announces Discovery Linking Key Cancer Cell Signaling Pathways With Proliferation and Self-Renewal of Human Embryonic Stem Cells
5. European Centre for Modern Drug Discovery Established in Hamburg
6. Dow AgroSciences and Exelixis Plant Sciences Announce Major Research Collaboration, Asset Purchase to Advance Gene Discovery and Validation
7. Discover Testing Settles the Drinking Water Debate: Tap vs. Bottled
8. Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases
9. Indivumed Supports Roche Diagnostics With Immunohistochemistry Services and Clinical Specimens for Biomarker Discovery and Drug Development
10. University of Leicester scientists discover technique to help friendly bacteria
11. Scientists discover how cancer may take hold
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/10/2017)... ... October 10, 2017 , ... USDM Life Sciences ... the life sciences and healthcare industries, announces a presentation by Subbu Viswanathan and ... presentation, “Automating GxP Validation for Agile Cloud Platforms,” will present a revolutionary approach ...
(Date:10/9/2017)... ... October 09, 2017 , ... At its national board meeting in ... professor in Harvard University’s Departments of Physics and Astronomy, has been selected for membership ... the winning team for the 2015 Breakthrough Prize in Fundamental physics for the discovery ...
(Date:10/7/2017)... (PRWEB) , ... October 06, ... ... leader in Hi-C-based genomic technologies, launched its ProxiMeta™ Hi-C metagenome deconvolution product, ... Hi-C kit and accompanying cloud-based bioinformatics software to perform Hi-C metagenome deconvolution ...
(Date:10/5/2017)... ... 2017 , ... LabRoots , the leading provider of educational and interactive ... back to cancer research with a month-long promotion supporting the advancement of breast cancer ... can use promo code PinkRibbon to get 10 percent off their purchase of every ...
Breaking Biology Technology:
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced ... will feature emerging and evolving technology through ... Innovation Summits will run alongside the expo portion of ... sessions, panels and demonstrations focused on trending topics within ... advanced design and manufacturing event will take place June ...
(Date:4/11/2017)... -- NXT-ID, Inc. (NASDAQ:   NXTD ) ("NXT-ID" ... of independent Directors Mr. Robin D. Richards and ... furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive Officer ... guidance and benefiting from their considerable expertise as we move ...
(Date:4/4/2017)...   EyeLock LLC , a leader of iris-based ... Patent and Trademark Office (USPTO) has issued U.S. Patent ... an iris image with a face image acquired in ... 45 th issued patent. "The ... the multi-modal biometric capabilities that have recently come to ...
Breaking Biology News(10 mins):