Navigation Links
Biologists discover an extra layer of protection for bacterial spores

Bacterial spores, the most resistant organisms on earth, carry an extra coating of protection previously undetected, a team of microbiologists reports in the latest issue of the journal Current Biology. Their findings offer additional insight into why spores of the bacteria that cause botulism, tetanus, and anthrax survive methods to eradicate them.

The study was conducted by researchers at New York University's Center for Genomics and Systems Biology, Loyola (Ill.) University's Medical Center, and Princeton University's Department of Molecular Biology.

The researchers studied the spores of a non-pathogenic bacterium, Bacillus subtilis, which is commonly found in soil. Although non-pathogenic, B. subtilis spores exhibit many of the same structural features of the spore-forming pathogens. In this study, the scientists examined the proteins that comprise spores' protective layers. Previous research has shown that 70 different proteins make up these layers. Less understood is how these proteins interact to form the spores' protective coats.

To do this, the researchers examined coat formation of both normal and mutant spores. In the latter case, they removed genes for selected coat proteins, allowing them to determine which proteins were necessary inand extraneous tothe formation of the spores' coats.

To observe proteins' behavior in living cells, the researchers fused the genes encoding the spores' coat proteins to a marker, a Green Fluorescent Protein (GFP). This procedure allowed them to monitor how the proteins localized to form spores' protective coats. A combination of fluorescence microscopy experiments and high-resolution image analysis enabled the researchers to overcome a theoretical limitation of light microscopy, pinpoint the location of the spores' coat proteins with a high degree of precision, and build a map of the spore coat. These experiments suggested the existence of a new outermost layer of the spore coat. They were then able to confirm the existence of this new layer using electron microscopy.

The researchers named this coat layer, located on the spores' outer surface, the "spore crust." While it has not yet been confirmed, it is possible that the spore crust is a common feature of all spore-forming bacteria, such as the botulism, tetanus, and anthrax pathogens.


Contact: James Devitt
New York University

Related biology technology :

1. Bringing Statistics to Biologists - New Genedata Analyst Launches at Society of Toxicology 49th Annual Meeting
2. Vermillion and Collaborators Discover Protein Biomarker Associated with Peripheral Artery Disease
3. PDL BioPharma Announces Significant Strategic and Portfolio Changes to Focus on Antibody Discovery and Development
4. Novocell Announces Discovery Linking Key Cancer Cell Signaling Pathways With Proliferation and Self-Renewal of Human Embryonic Stem Cells
5. European Centre for Modern Drug Discovery Established in Hamburg
6. Dow AgroSciences and Exelixis Plant Sciences Announce Major Research Collaboration, Asset Purchase to Advance Gene Discovery and Validation
7. Discover Testing Settles the Drinking Water Debate: Tap vs. Bottled
8. Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases
9. Indivumed Supports Roche Diagnostics With Immunohistochemistry Services and Clinical Specimens for Biomarker Discovery and Drug Development
10. University of Leicester scientists discover technique to help friendly bacteria
11. Scientists discover how cancer may take hold
Post Your Comments:
(Date:11/24/2015)... 24, 2015 --> ... report "Oligonucleotide Synthesis Market by Product & Services (Primer, ... Diagnostic, DNA, RNAi), End-User (Research, Pharmaceutical & Biotech, Diagnostic ... the market is expected to reach USD 1,918.6 Million ... a CAGR of 10.1% during the forecast period. ...
(Date:11/24/2015)... 24, 2015  Tikcro Technologies Ltd. (OTCQB: TIKRF) today announced that its ... at 11:00 a.m. Israel time, at the law ... Allon Street, 36 th Floor, Tel Aviv, Israel ... and Izhak Tamir to the Board of Directors; ... directors; , approval of an amendment to certain terms of options ...
(Date:11/24/2015)... ... November 24, 2015 , ... Creation Technologies would like to ... Deloitte's 2015 Technology Fast 500 list of the fastest growing companies in North ... II medical device that speeds up orthodontic tooth movement by as much as ...
(Date:11/24/2015)... , November 24, 2015 , ... in a European healthcare ... the companies will work closely together in identifying European breakthrough ... medical need. The collaboration is underpinned by a significant investment ... This is the first investment by Bristol-Myers Squibb in a ...
Breaking Biology Technology:
(Date:10/29/2015)... 2015   MedNet Solutions , an innovative SaaS-based ... clinical research, is pleased to announce that it has ... as one of only three finalists for a ... and Growing" category. The Tekne Awards honor Minnesota ... technology innovation and leadership. iMedNet™ eClinical ...
(Date:10/29/2015)... 2015 NXTD ) ("NXT-ID" ... on the growing mobile commerce market and creator ... a leading marketplace to discover and buy innovative ... wallet on StackSocial for this holiday season.   ... "Company"), a biometric authentication company focused on the ...
(Date:10/26/2015)... 26, 2015 ... adds Biometrics Market Shares, Strategies ... well as Emerging Biometrics Technologies: Global ... its collection of IT and Telecommunications ... --> . ...
Breaking Biology News(10 mins):