Navigation Links
Biocept Demonstrates Multiple Phenotypes of Circulating Tumor Cells (CTCs) in Cancer Patients
Date:5/10/2011

SAN DIEGO, May 10, 2011 /PRNewswire/ -- Biocept, Inc., a privately-held laboratory testing company focused on the detection, enumeration and analysis of circulating tumor cells (CTCs) in cancer patients, today announced the publication of a paper describing the identification of different CTC phenotypes using its proprietary Cell Enrichment and Extraction (CEE™) platform.  The paper, appearing in the Journal of Oncology and entitled "Detection of EPCAM-Negative and Cytokeratin-Negative CTCs in Peripheral Blood", supports a growing belief that there are many cancer patients with CTCs that don't meet the standard criteria by which a CTC is defined and, importantly, detected in current practice.  The Biocept technology platform may allow more sensitive and comprehensive CTC tests for cancer patients.

David Hale, Executive Chairman of Biocept, said, "We are very excited with these results, as they support the potential of Biocept's CEE™ platform, not only to provide more sensitivity than current methods and detect more CTCs from the same blood sample, but also to derive more information about, and offer new insights into, a patient's cancer."  He continued, "We are very optimistic that better treatment decisions, and, ultimately, better patient outcomes, will result."  

CTCs are very rare in the blood of cancer patients, ranging from 1 to over a thousand in a standard 10 ml blood sample, which may contain 50-100 billion normal red or white blood cells.  To be able to count or analyze CTCs, an enrichment process is necessary.  Enrichment from blood is typically achieved using antibodies to EpCAM (Epithelial Cell Adhesion Molecule), a protein found on the surface of epithelial cells that enables them to bind to each other and to the extracellular matrix.  CTCs are normally detected after capture using antibodies to cytokeratin (CK), an intracellular structural protein typically found in epithelial cells.  However, EpCAM and CK are not expressed in all tumor cells of epithelial origin, nor in many other tumor types such as sarcomas and malignant melanomas. EpCAM and CK can also be down-regulated or absent in tumor cells undergoing the epithelial-to-mesenchymal (EMT) transition, a process by which tumor cells become less rigid and more pliable, presumably facilitating their migration into blood vessels and capillaries to establish metastases.

With the CEE™ platform Biocept's scientists used an antibody mixture, or cocktail, in addition to an anti-EpCAM antibody, for CTC capture. These antibodies were directed to a range of cell surface antigens, some more typically seen on epithelial-type cells, and others on mesenchymal-type cells.  They also used CEE-Enhanced™ staining (CEE-E) for CTC detection, which is a unique in situ staining method that fluorescently labels the capture antibodies bound to CTCs. This enables the detection of all specifically captured CTCs, including those that are CK-negative.   Biocept scientists evaluated blood samples from 24 stage IV metastatic breast cancer patients, and demonstrated that after capture with the antibody cocktail, 15 of 24  (63%; range 1-60 CTCs) stained positively with anti-CK antibodies, and all 24 were positive for additional CK-negative, CEE-E positive CTCs (100%; range 1-41; median=11). HER2 FISH was performed on the captured cells, and both CK-positive and CK-negative/CEE-E positive cells were found with amplified HER2 genes, confirming them as tumor cells.  A key finding from this study is that the peripheral blood of cancer patients contains CTCs different from those captured and detected with standard approaches employing only antibodies to EpCAM and CK, (i.e., CK- negative, CEE-E positive).  

Stephen Mikolajczyk, Senior Director of R&D and principal scientist at Biocept and lead author on the paper, commented, "We think it is important for the cancer community to recognize that CTCs are heterogeneous, like the tumors they come from, and focusing on a single CTC sub-population, such as an EpCAM+/CK+ phenotype, excludes cells from analysis that may contain valuable information that is highly relevant for some patients."

Biocept's CEE™ Platform

The CEE™ (cell enrichment and extraction) platform and the family of associated specialized laboratory oncology tests (OncoCEE™) will be provided as a service to physicians, pharmaceutical and biotechnology companies through Biocept's CLIA-certified, CAP-accredited laboratory.  The CEE™ platform is comprised of micro-fluidic capture channels and associated instrumentation, the antibody capture cocktail, CEE-Enhanced™ staining technology, and biomarker assays performed on the CTCs.  Biocept plans to launch its first commercial product, Onco-CEE-BR™ for breast cancer, in Q3, 2011.

About Biocept, Inc.

Biocept Laboratories, headquartered in San Diego, California, is a diagnostic and advanced laboratory services company specializing in the capture, detection, isolation and molecular analysis of circulating tumor cells (CTCs). Biocept's mission is to enhance the lives of cancer patients through the development of innovative diagnostic products and services. Biocept utilizes patented and innovative technologies to deliver clinically relevant information to physicians that enable better patient care. This includes clinical assessments of CTCs, both prognostic and predictive, which may provide physicians with information highly relevant for the treatment of their patients with cancer.

Web site: http://www.biocept.com/


'/>"/>
SOURCE Biocept, Inc.
Copyright©2010 PR Newswire.
All rights reserved

Related biology technology :

1. Biocept, Inc. Announces New Director Appointment - September 28, 2010
2. Biocept, Inc. Announces Two New Director Appointments
3. Biocept, Inc. Announces CEO and Director Appointments
4. Dune Medicals MarginProbe™ Demonstrates Increased Positive Margin Identification Leading to Reduction in Patients Indicated for Re-excision in Landmark Lumpectomy Surgery Trial
5. Precision Therapeutics ChemoFx® Demonstrates Significant Role in Selection of Chemotherapy Doublets in Non Small Cell Lung Cancer
6. Otonomys OTO-104 Demonstrates Hearing Loss Protection and Hearing Recovery in Preclinical Studies
7. Capasso lab demonstrates highly unidirectional whispering gallery microlasers
8. Integrated Analysis Demonstrates Alpha(1)-Augmentation Therapy Significantly Reduces Loss of Lung Tissue in Patients with AAT-deficiency-related Emphysema
9. Molecular Detections Detect-Ready™ MRSA Assay Demonstrates Strong Performance In Independent Comparative Study From Leading UK Hospital
10. Newly Published Caldolor® Clinical Data Demonstrates 77% Reduction in Fever in Hospitalized Patients
11. Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/23/2016)... ... June 23, 2016 , ... UAS ... the launch of their brand, UP4™ Probiotics, into Target stores nationwide. The company, ... proud to add Target to its list of well-respected retailers. This list includes ...
(Date:6/23/2016)... 23, 2016 /PRNewswire/ - FACIT has announced the ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or ... of a portfolio of first-in-class WDR5 inhibitors for ... as WDR5 represent an exciting class of therapies, ... medicine for cancer patients. Substantial advances have been ...
(Date:6/23/2016)... , June 23, 2016 Houston ... with the Cy-Fair Sports Association to serve as ... the agreement, Houston Methodist Willowbrook will provide sponsorship ... and connectivity with association coaches, volunteers, athletes and ... with the Cy-Fair Sports Association and to bring ...
(Date:6/23/2016)... , June 23, 2016  The Prostate Cancer Foundation (PCF) ... precise treatments and faster cures for prostate cancer. Members of the Class of ... 15 countries. Read More About the Class of 2016 ... ... ...
Breaking Biology Technology:
(Date:6/21/2016)... 21, 2016 NuData Security announced today that ... of principal product architect and that Jon ... customer development. Both will report directly to ... moves reflect NuData,s strategic growth in its product ... customer demand and customer focus values. ...
(Date:6/9/2016)... Paris Police Prefecture and ... ensure the safety of people and operations in several locations ... Teleste, an international technology group specialised in broadband ... its video security solution will be utilised by ... across the country. The system roll-out is scheduled for the ...
(Date:6/2/2016)... Perimeter Surveillance & Detection Systems, Biometrics & ... & Other Service  The latest report from ... of the global Border Security market . Visiongain ... billion in 2016. Now: In November 2015 ... and hardware technologies for advanced video surveillance. ...
Breaking Biology News(10 mins):