Navigation Links
Bacterial Identification System Differentiates Virulent “Big 6” E. Coli Strains in Five Hours
Date:6/5/2013

Woburn, MA (PRWEB) June 05, 2013

PathoGenetix, Inc., developer of an automated system for rapid bacterial identification, has presented new research demonstrating the use of Genome Sequence Scanning™ (GSS™) to identify and differentiate virulent Shiga toxin-producing E. coli (STEC) strains directly from enriched food samples in five hours. The results, which were detailed in a poster presented at the Association of Public Health Laboratories (APHL) Annual Meeting on Sunday, have significant implications for food producers required to test their products for pathogens with certain virulence characteristics, and for public health agencies needing to quickly identify and find the source of foodborne illness outbreaks.

The research tested representative strains of the pathogenic “Big 6” STECs, which contained the stx and eae virulence genes, as well as non-STEC Big 6 E. coli, on PathoGenetix’s Genome Sequence Scanning system. GSS successfully identified and differentiated each serovar of the Big 6 STEC E. coli. In addition, preliminary data showed the potential to differentiate Big 6 STECs from Big 6 E. coli of the same serotype that do not possess the stx and/or eae virulence genes. The current criteria for determining the presence of a Big 6 pathogen is the identification of the presence of both of these virulence genes in a Big 6 serovar.

The most common STEC serotype is O157:H7, which food producers have been testing for since 1994. Last year, the U.S. Department of Agriculture Food Safety and Inspection Service (USDA-FSIS) added six additional pathogenic E. coli serotypes (O26, O45, O103, O111, O121 and O145) to its list of food adulterants that it requires meat producers to test for in certain beef products.

Current screening methods have a high “false positive” rate for virulence due to the commingling of virulence factors that can be present in an enriched food sample. The ability of GSS to reliably differentiate virulent STECs means that the system could be used as a confirmatory method for Big 6 STEC screening assays, and potentially reduce the loss of product associated with false positive results from current STEC screening tests.

The automated GSS system extracts, scans and analyzes microbial DNA directly from a complex sample to provide bacterial serotype and strain type results in five hours. The bacterial strain information provided by GSS is comparable to pulsed field gel electrophoresis (PFGE), the current standard for pathogen typing in foodborne illness outbreak investigations. Unlike PFGE however, GSS does not require pure culture isolates and thus is compatible with newer pathogen detection methods in use in clinical and food industry laboratories. Genome Sequence Scanning will be commercially available in 2014 as the RESOLUTION™ Microbial Genotyping System.

About PathoGenetix, Inc.

PathoGenetix, Inc. is a commercial-stage developer of an automated system for rapid bacterial identification from complex samples. PathoGenetix is a venture-backed company that has received more than $50 million in technology development funding from the Department of Homeland Security. The core GSS technology isolates and analyzes DNA directly from an enriched biological sample—without the need for a cultured isolate—and provides results in just five hours, days faster than current methods. GSS has broad applicability in food safety, industrial microbiology, and clinical diagnostics and research. The first commercial GSS system will be available in 2014 for use in food safety testing and foodborne illness outbreak investigations. Learn more at http://www.pathogenetix.com.

Read the full story at http://www.prweb.com/releases/APHL/Ecoli_STEC/prweb10802733.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. Seeing the Future: How a Revolutionary New Bacterial Screening Device Can Predict a Patient’s Future for Tooth Decay
2. Seeing the Future: How a Revolutionary New Bacterial Screening Device Can Predict a Patient’s Future for Tooth Decay
3. Cempra Presents Post-Phase 2 Analysis of Solithromycins Efficacy and Safety Results from Patients with Community Acquired Bacterial Pneumonia (CABP)
4. Seeing the Future: How a Revolutionary New Bacterial Screening Device Can Predict a Patient’s Future for Tooth Decay
5. Seeing the Future: How a Revolutionary New Bacterial Screening Device Can Predict a Patient’s Future for Tooth Decay
6. PathoGenetix Advances Commercialization of New RESOLUTION Microbial Genotyping System for Rapid Bacterial Serotyping and Strain Typing
7. Synedgen Announces Effective Treatment Against Bacterial Biofilms in Minor Animal Species
8. PathoGenetix and FDA to Evaluate Bacterial Identification System for Use in FDA Public Health and Food Safety Efforts
9. Seeing the Future: How a Revolutionary New Bacterial Screening Device can Predict a Patient’s Future for Tooth Decay
10. Seeing the Future: How a Revolutionary New Bacterial Screening Device Can Predict a Patient’s Future for Tooth Decay
11. Seeing the Future: How a Revolutionary New Bacterial Screening Device can Predict a Patient’s Future for Tooth Decay
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... June 27, 2016 , ... Parallel 6 , the ... today the Clinical Reach Virtual Patient Encounter CONSULT module which enables both ... physician and clinical trial team. , Using the CONSULT module, patients and physicians can ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf ... join the faculty of the University of North Carolina Kenan-Flagler Business School ... and entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s international efforts, ...
(Date:6/27/2016)... --  Ginkgo Bioworks , a leading organism design ... awarded as one of the World Economic Forum,s ... innovative companies. Ginkgo Bioworks is engineering biology to ... in the nutrition, health and consumer goods sectors. ... including Fortune 500 companies to design microbes for ...
(Date:6/24/2016)... , June 24, 2016 Epic ... sensitively detects cancers susceptible to PARP inhibitors by ... tumor cells (CTCs). The new test has already ... therapeutics in multiple cancer types. Over ... DNA damage response pathways, including PARP, ATM, ATR, ...
Breaking Biology Technology:
(Date:6/20/2016)... 2016 Securus Technologies, a leading provider ... public safety, investigation, corrections and monitoring announced that ... has secured the final acceptance by all three ... Access Systems (MAS) installed. Furthermore, Securus will have ... installed by October, 2016. MAS distinguishes between legitimate ...
(Date:6/15/2016)... , June 15, 2016 ... market report titled "Gesture Recognition Market by Application Market - Global ... 2016 - 2024". According to the report, the  global ... billion in 2015 and is estimated to grow ... 48.56 billion by 2024.  Increasing application ...
(Date:6/9/2016)... Finland , June 9, 2016 ... National Police deploy Teleste,s video security solution to ensure the ... France during the major tournament ... data communications systems and services, announced today that its video ... Prefecture to back up public safety across the ...
Breaking Biology News(10 mins):