Navigation Links
Argonne Researchers Develop Method that Aims to Stabilize Antibodies
Date:9/3/2009

. These chains are made up of modules known as constant and variable domains. The light and heavy chain each has a variable domain, which come together to form the antigen binding site. Because of the great diversity of amino acids in the variable domains, different antibodies are capable of interacting with an effectively unlimited number of targets.

Sometimes this variability comes at a price; the amyloid-forming light chains were less stable than their normal counterparts. However, even amyloid-forming light chains have amino acid substitutions that improve stability. When seven of these amino acid changes were introduced into an amyloid-forming variable domain, a billion-fold improvement in thermodynamic stability was obtained reflecting a much higher ratio of native protein folds to unfolded proteins - a major determinate of antibody shelf life.

"Our work at this detailed level has taught us that antibody stabilization was possible, but we needed to find out if antibodies could be stabilized without compromising their function and do so with moderate experimental investment," Stevens said. Recent work suggests these goals are potentially achievable. To proactively improve the stability of a different antibody variable domain, Argonne researchers drew up a short list of 11 candidate amino acid changes. Four of the amino acid changes improved antibody stability and when combined together in the original domain provided a 2,000-fold improvement in stability.

A follow up experiment using a functional antibody fragment was able to improve antibody stability comparably, with no loss of antibody functionality. Both experiments required approximately one month to accomplish instead of the potentially open-ended time required for most protein stabilization projects.

There is a correlation between thermodynamic stability and thermal stability, the billion fold improvement in thermodynamic stability increased the therm
'/>"/>

Source: PRWeb
Copyright©2009 Vocus, Inc.
All rights reserved  

Page: 1 2 3 4

Related biology technology :

1. Argonne researchers win 2 R&D 100 Awards
2. Argonnes Lithium-Ion Battery Technology to be Commercialized by Japans Toda Kogyo
3. Argonne researcher studies what makes quantum dots blink
4. Argonne scientist to give the plenary talk at Royal Society of Chemistry meeting on nanoalloys
5. Researchers design new graphene-based, nano-material with magnetic properties
6. ChemBridge Corporation and Collaborative Drug Discovery Announce Strategic Collaboration to Provide Diverse Chemical Libraries Together With Researchers Own Private Data
7. Dartmouth researchers propose new way to reproduce a black hole
8. Nucleix Researchers Discover DNA Evidence May Easily be Falsified: Company Develops New Detection Technology for Preventing Biological Identity Theft
9. K-States Biosecurity Research Institute Gives Researchers the Tools Needed to Study Diseases That Cost Pig Producers Hundreds of Millions of Dollars Each Year
10. Sigma-Aldrich Acquires ChemNavigator, Inc., to Provide Researchers With Industry-Leading Chemical Compound Selection and Procurement Services
11. UCSF researchers help crack parasite genome, identify drug leads
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Argonne Researchers Develop Method that Aims to Stabilize Antibodies 
(Date:8/27/2015)... ... August 27, 2015 , ... Inc. Magazine released its annual list ... as number 1,361 in growth for the three years through 2014. Being named to ... privately held organizations in the country. , “We are thrilled to make the ...
(Date:8/27/2015)... 27, 2015 In response to recent ... and The US Postal Service to no longer ... ,select agents, by the CDC, Marken reaffirmed today ... sensitive shipments. Marken uses its flexible ... and certified personnel to ensure the safe and ...
(Date:8/26/2015)... ... August 26, 2015 , ... ... today that a second US laboratory is to open in Manhattan, Kansas in ... continued partnership and long-term growth of research and development through collaboration with researchers ...
(Date:8/26/2015)... N.J. , Aug. 26, 2015  Roka Bioscience, ... focused on providing advanced testing solutions for the detection ... CEO will present at the Sidoti & Company Emerging ... conference will be held at the New York Marriott ... presentation will be available through the investor relations section ...
Breaking Biology Technology:iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3Marken Ensures Safe Transportation Of Select Agents And Toxins 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3
... , Jason S. Wood, Varian, ... , Cytochrome P450s (CYPs) ... human body 1 . CYPs are part of the Phase I ... the metabolized drug enabling either easy elimination of the drug or ...
... Z. Yang and S. Sadjadi, Varian, Inc. , ... Amphetamine drugs are often abused and misused in ... and doping laboratories are frequently asked to analyze for the presence ... sample volumes can be collected non-invasively. These drugs generally remain detectable ...
... cells through the B cell receptor or T cells through the ... different members of the same kinase families are used. ZAP-70 and ... and structures. Crosslinking of the CD3 receptor complex leads to the ... 1,2 . These kinases phosphorylate ITAM regions on the receptor which ...
Cached Biology Technology:Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method 2Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method 3Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS 2Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS 3Research Tools for Analysis of ZAP-70 2Research Tools for Analysis of ZAP-70 3
(Date:8/12/2015)... , August 12, 2015 ... of mobile payment innovation and advanced biometrics technology is ... the way consumers rely on using their credit cards, ... reinventing the future for payment services led by companies ... NXTD ), :  Google, Inc. (NASDAQ: GOOG ), ...
(Date:8/11/2015)... Today, ZUK announced its Android smartphone Z1, featuring FPC,s touch ... 2015 that relate to sales of FPC1155 for this smartphone model ... for 2015. Jörgen Lantto, CEO of FPC, comments: " ... manufacturer in China and we are proud ... for Z1 , its first ...
(Date:8/6/2015)... 6, 2015  Today, U.S. Congressman Mike ... ™ to learn firsthand how Silicon Valley technologies ... is a world leader in multi-factor identity management ... helping federal agencies and commercial organizations achieve new ... "The vast majority of network breaches can ...
Breaking Biology News(10 mins):Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 4Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 6FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2Rep. Honda to Visit Crossmatch in Redwood City 2
... have had an accelerated childhood compared to that of modern ... by researchers from Ohio State University and the University of ... tooth growth present in the Neanderthal fossils they examined was ... , And since the rate of tooth growth has ...
... testing methods helped public health officials control and limit ... A virus in 2003 related to the consumption of ... the October 15 issue of The Journal of Infectious ... study, Joseph J. Amon, PhD, MSPH, and colleagues at ...
... testing methods helped public health officials control and limit ... A virus in 2003 related to the consumption of ... the October 15 issue of The Journal of Infectious ... study, Joseph J. Amon, PhD, MSPH, and colleagues at ...
Cached Biology News:Neanderthal teeth grew no faster than comparable modern humans' 2Neanderthal teeth grew no faster than comparable modern humans' 3Disrupting Cocaine-memories To Battle Addiction 2Large-scale Computer Simulations Reveal New Insights Into Antibiotic Resistance 2
... rat tissue extracts (liver, kidney, spleen, and heart) ... extract. It was also tested with microsomal fractions ... A simple and reliable method for measuring NDPase ... of the inorganic phosphate released from NDP by ...
Compound(s) tested through a broad panel of 38 ligand binding and enzyme assays targeting the Central Nervous System. Used to determine selecitivty and potential CNS-Side Effect liabilities of compo...
... through 63 ligand binding ... a broad therapeutic range. ... many non human tissue ... assays. Used to ...
... Receptor (PR) Competitor Assay Kits are ... progesterone receptor binding compounds using fluorescence ... a fusion of glutathione transferase to ... progesterone receptor [PR-LBD(GST)] and a proprietary, ...
Biology Products: