Navigation Links
Argonne Researchers Develop Method that Aims to Stabilize Antibodies
Date:9/3/2009

. These chains are made up of modules known as constant and variable domains. The light and heavy chain each has a variable domain, which come together to form the antigen binding site. Because of the great diversity of amino acids in the variable domains, different antibodies are capable of interacting with an effectively unlimited number of targets.

Sometimes this variability comes at a price; the amyloid-forming light chains were less stable than their normal counterparts. However, even amyloid-forming light chains have amino acid substitutions that improve stability. When seven of these amino acid changes were introduced into an amyloid-forming variable domain, a billion-fold improvement in thermodynamic stability was obtained reflecting a much higher ratio of native protein folds to unfolded proteins - a major determinate of antibody shelf life.

"Our work at this detailed level has taught us that antibody stabilization was possible, but we needed to find out if antibodies could be stabilized without compromising their function and do so with moderate experimental investment," Stevens said. Recent work suggests these goals are potentially achievable. To proactively improve the stability of a different antibody variable domain, Argonne researchers drew up a short list of 11 candidate amino acid changes. Four of the amino acid changes improved antibody stability and when combined together in the original domain provided a 2,000-fold improvement in stability.

A follow up experiment using a functional antibody fragment was able to improve antibody stability comparably, with no loss of antibody functionality. Both experiments required approximately one month to accomplish instead of the potentially open-ended time required for most protein stabilization projects.

There is a correlation between thermodynamic stability and thermal stability, the billion fold improvement in thermodynamic stability increased the therm
'/>"/>

Source: PRWeb
Copyright©2009 Vocus, Inc.
All rights reserved  

Page: 1 2 3 4

Related biology technology :

1. Argonne researchers win 2 R&D 100 Awards
2. Argonnes Lithium-Ion Battery Technology to be Commercialized by Japans Toda Kogyo
3. Argonne researcher studies what makes quantum dots blink
4. Argonne scientist to give the plenary talk at Royal Society of Chemistry meeting on nanoalloys
5. Researchers design new graphene-based, nano-material with magnetic properties
6. ChemBridge Corporation and Collaborative Drug Discovery Announce Strategic Collaboration to Provide Diverse Chemical Libraries Together With Researchers Own Private Data
7. Dartmouth researchers propose new way to reproduce a black hole
8. Nucleix Researchers Discover DNA Evidence May Easily be Falsified: Company Develops New Detection Technology for Preventing Biological Identity Theft
9. K-States Biosecurity Research Institute Gives Researchers the Tools Needed to Study Diseases That Cost Pig Producers Hundreds of Millions of Dollars Each Year
10. Sigma-Aldrich Acquires ChemNavigator, Inc., to Provide Researchers With Industry-Leading Chemical Compound Selection and Procurement Services
11. UCSF researchers help crack parasite genome, identify drug leads
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Argonne Researchers Develop Method that Aims to Stabilize Antibodies 
(Date:1/14/2014)... January 14, 2014 Date: Friday, April 11, ... Warrington Country Club, 1360 Almshouse Road, Warrington, Pa. ... organization solely dedicated to finding a cure for hepatitis B ... will host its annual Crystal Ball on Friday, April 11 ...
(Date:1/14/2014)... Communications, a leading provider of strategic communications services to corporations and ... the United States and Europe ... JD, is returning to the firm,s Washington, D.C. ... more than two years of service as Associate Commissioner for the ...
(Date:1/14/2014)... The largest international professional organization of scientists ... derivatives thereof has endorsed an educational program that ... challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural Product ...
(Date:1/14/2014)... In recent years, growing suspicion about ... product development and promotion has led to unprecedented levels ... by concerns about the insidious impact of commercialization of ... fines to the world’s biggest pharmas for illegal marketing ...
Breaking Biology Technology:Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3
... PHILADELPHIA, May 28 Premier Research Group plc ... of Anthony Faragasso, MD as Executive Director and ... position, Dr. Faragasso will lead,the global Medical Management ... and safety services, Dr. Faragasso brings to ...
... ... Manufacturing Experience -, SAN DIEGO, May 28 ... Gordon H.,Busenbark to the company,s board of directors. Mr. Busenbark ... privately,held development-stage specialty pharmaceuticals company focused on,diseases of the central ...
... launch of a new collaborative website initially focusing on ... WikiProfessional technology underlying the site has been developed based ... open access journal Genome Biology, WikiProteins provides a method ... The article is written by Barend Mons of the ...
Cached Biology Technology:Premier Research Names Anthony Faragasso, MD as Executive Director and Global Head of Medical Management and Safety 2Nventa Appoints Gordon H. Busenbark to Board of Directors 2Large-scale community protein annotation -- WikiProteins 2
(Date:7/9/2014)... a sparrow-sized fossil from China challenges the commonly held ... gained the ability to fly. The birdlike fossil is ... rather the remains of a tiny tree-climbing animal that ... Dinosaur Museum in Blanding, Utah, and Alan Feduccia of ... Springer,s Journal of Ornithology . , The fossil ...
(Date:7/9/2014)... of the amphibians with the highest distribution in the ... water where it comes into contact with the red ... out by the Spaniard Germn Orizaola from the University ... frogs have developed a defensive response to the invasive ... if they co-exist with the crayfish. , Numerous invasive ...
(Date:7/9/2014)... measurement results accrue. To completely and systematically archive ... In fact, researchers in the life sciences spend ... to an online survey of 70 people working ... for Applied Information Technology FIT in Sankt Augustin. ... no centralized or structured approach to data collection ...
Breaking Biology News(10 mins):Researchers declassify dinosaurs as being the great-great-grandparents of birds 2Frogs have developed rapid defences against the red swamp crayfish 2Frogs have developed rapid defences against the red swamp crayfish 3Managing the data jungle 2Managing the data jungle 3
... The replanting of mangroves on the coasts of the Philippines ... 20-30 typhoons that hit the islands annually. This is ... immediate and massive mangrove replanting, that J.H. Primavera from the ... La Salle University, both in the Philippines, cite in a ...
... billion tons of microbial organismsexpressed in terms of carbon ... published online by Nature , July 20, 2008. ... of carbon stored globally in tropical rainforests. The authors: ... Marine Environmental Sciences (MARUM) at University of Bremen, Germany; ...
... of the nickel metal hydride (NiMH) technology used for ... hybrid gas-electric cars believes the hydrogen economy is already ... the current issue of the International Journal of ... and CEO of Ovshinsky Innovation LLC, based in Bloomfield ...
Cached Biology News:Mangroves key to saving lives 2Nature publishes new evidence about the deep biosphere written by biogeoscientists 2Nature publishes new evidence about the deep biosphere written by biogeoscientists 3Closing the hydrogen economic loop 2
Request Info...
... the optimization of immunostaining. ... the primary antibody, selection and ... Alk. Phos., HRP, fluorescence, or ... evaluation by light or fluorescence ...
Ultra-sensitive, non-radioactive detection of Protein G. Measure Protein G levels quickly and easily....
Request Info...
Biology Products: