It's been used to dye the Chicago River green on St. Patrick's Day. It's been used to find latent blood stains at crime scenes. And now researchers at Northwestern University have used it to examine the thinnest material in the world.
The useful tool is the dye fluorescein, and Jiaxing Huang, assistant professor of materials science and engineering at the McCormick School of Engineering and Applied Science, and his research group have used the dye to create a new imaging technique to view graphene, a one-atom thick sheet that scientists believe could be used to produce low-cost carbon-based transparent and flexible electronics.
Their results were recently published in the Journal of the American Chemical Society.
Being the world's thinnest materials, graphene and its derivatives such as graphene oxide are quite challenging to see. Current imaging methods for graphene materials typically involve expensive and time-consuming techniques. For example, atomic force microscopy (AFM), which scans materials with a tiny tip, is frequently used to obtain images of graphene materials. But it is a slow process that can only look at small areas on smooth surfaces. Scanning electron microscopy (SEM), which scans a surface with high-energy electrons, only works if the material is placed in vacuum. Some optical microscopy methods are available, but they require the use of special substrates, too.
"There are really no good techniques that are general enough to meet the diverse imaging needs in the research and development of this group of new materials," Huang says. "For example, people have proposed putting graphene materials on plastic sheets for flexible electronics, but seeing them on plastic has been very challenging. If one cannot exam these materials, quality control is going to be difficult."
Fluorescent labeling has been used routinely to image biological samples, typically by using fluorescent dyes that make the obj
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