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AMRESCO’s New VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits Offer Long-lasting, High Sensitivity Detection
Date:3/31/2013

Solon, Ohio (PRWEB) March 31, 2013

Chemiluminescent substrates are standard reagents for the detection of horseradish peroxidase (HRP) in Western blotting. Not all substrates perform equally, however. Critical characteristics to consider for chemiluminescent substrate selection are the sensitivity required to detect a target of interest, the stability of the signal generated and the ability to quantify the signal. AMRESCO’s VisiGlo™ HRP Chemiluminescent Substrate Kit with picogram sensitivity is now one of three VisiGlo™ options available after the addition of VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits to their repertoire. The different formulations, from low to high sensitivity, allow HRP detection across a broad range of Western blotting conditions.

AMRESCO's new VisiGlo™ Prime HRP Chemiluminescent Substrate Kit is ideal for accurate protein quantitation, with a linear dynamic range of signal versus protein concentration encompassing over three orders of magnitude. The substrate has attomolar sensitivity and can detect both low and high abundance protein on a single blot exposure without exhibiting substrate depletion, a phenomenon observed as inverse staining of highly concentrated protein. The chemiluminescent emission from VisiGlo™ Prime is highly stable, lasting hours after substrate incubation to allow time for optimizing exposures. VisiGlo™ Prime is recommended for use with CCD imagers or film.

VisiGlo™ Select HRP Chemiluminescent Substrate Kit combines a broad linear dynamic range and low femtogram sensitivity to enable quantitation of low-abundance targets that other substrates may not reveal. The sensitivity and high signal-to-noise ratio permit target detection with minimal consumption of valuable protein and antibody reagents and allow long exposure times witho
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