Navigation Links
AMRESCO’s New Fluorescent DNA Quantitation Kit Uses a Highly Specific DNA-binding Dye that Allows Accurate Quantitation Even in Crude Extracts
Date:5/6/2013

Solon, Ohio (PRWEB) May 06, 2013

DNA quantitation is crucial to experimental design and interpretation of results, especially in sensitive molecular biology applications such as PCR, or for any comparative analysis of multi-source DNA samples. Although UV spectrophotometric measurement at 260 nm is the most widely practiced method of DNA quantitation, it is limited by high background interference from common impurities, such as RNA and protein. Better accuracy and sensitivity can be achieved using AMRESCO’s new Fluorescent DNA Quantitation Kit. The kit utilizes the fluorescent DNA intercalator, bisBenzimide H33258, otherwise known as Hoechst dye, and a simple method that may be easily scaled for a single cuvette or for high throughput in multi-well plates.

AMRESCO's Fluorescent DNA Quantitation kit includes Fluorescent DNA Dye Solution, Fluorescent DNA Standard and Fluorescent DNA Assay Buffer, 10X in volumes sufficient to perform up to 750 reactions. Assays are performed by fluorometric reading of a DNA standard series and unknown samples prepared in diluted Fluorescent DNA Dye Solution. The selective binding of the bisBenizimide H33258 dye to A-T base pairs results in significant fluorescent emission at 460 nm in the presence of DNA. The dye has low affinity for RNA, which improves specificity and sensitivity of the assay results when using impure samples. The assay also tolerates protein contamination that may be present in crude extracts and various buffer compositions commonly used in DNA extraction. The sensitivity of DNA detection with AMRESCO's Fluorescent DNA Quantitation Kit ranges from the low
'/>"/>

Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Page: 1 2

Related biology technology :

1. AMRESCO’s New VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits Offer Long-lasting, High Sensitivity Detection
2. ATCC Announces Agreement with Life Technologies Corporation to Sell and Distribute Green Fluorescent Protein (GFP)-based Materials
3. Goodbye, fluorescent light bulbs! See your office in a new light
4. Research on China's FISH Probe (Fluorescent in Situ Hybridization Probe) Industry, 2013-2017
5. UCLA team develops highly efficient method for creating flexible, transparent electrodes
6. Stanford engineers perfecting carbon nanotubes for highly energy-efficient computing
7. Researchers create highly conductive and elastic conductors using silver nanowires
8. Highly efficient production of advanced biofuel by metabolically engineered microorganism
9. Highly lethal Ebola virus has diagnostic Achilles heel for biothreat detection, scientists say
10. Medrio, a Leading EDC and eClinical SaaS Provider for Clinical Research, Announces the Highly Anticipated Release of Medrio 8.1 with iPad Support
11. New Resource Provides Herb Industry With Sources of Quality Specifications
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/21/2014)... With a larger, brighter interface, ... Cole-Parmer® Digital Gear Pump Drive for Micropump® ... for automated process applications. , Simply set time ... count function counts down and displays batches completed ... dispensed across multiple cycles. View the four operating ...
(Date:7/21/2014)... 2014 ResearchMoz presents this ... USA Cancer Biomaker Market to 2018 ... ResearchMoz presents this most up-to-date research on Global ... Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, ... GLOBAL & USA ...
(Date:7/18/2014)... 2014 Regeneron Pharmaceuticals, Inc. (NASDAQ: REGN) today announced ... and operating results on Tuesday, August 5, 2014, before ... a conference call and simultaneous webcast at 8:30 AM ... InformationTo access this call, dial (888) 660-6127 (U.S.) or ... be accessed from the ,Events and Presentations, page of ...
(Date:7/18/2014)... , July 18, 2014  Neurotrope, Inc. (OTCQB: NTRP) ... of Directors appointed Co-Chairmen Charles S. Ramat ... on an interim basis, effective immediately. Messrs. Ramat and ... no longer Chief Executive Officer of Neurotrope Bioscience, Inc., ... and whose employment agreement with Neurotrope Bioscience Inc. terminated ...
Breaking Biology Technology:New Easy-View Cole-Parmer Digital Gear Pump Drive Makes Performance Monitoring Effortless 2Global & USA Cancer Biomaker Market to 2018 (Identification, Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, Business Strategies, Industry Trends, and Pipeline Analysis) - Market Size, Trends, and Forecasts A 2Global & USA Cancer Biomaker Market to 2018 (Identification, Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, Business Strategies, Industry Trends, and Pipeline Analysis) - Market Size, Trends, and Forecasts A 3Global & USA Cancer Biomaker Market to 2018 (Identification, Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, Business Strategies, Industry Trends, and Pipeline Analysis) - Market Size, Trends, and Forecasts A 4Global & USA Cancer Biomaker Market to 2018 (Identification, Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, Business Strategies, Industry Trends, and Pipeline Analysis) - Market Size, Trends, and Forecasts A 5Global & USA Cancer Biomaker Market to 2018 (Identification, Technologies, Market Analysis, Competitor Profiles, Companion Diagnostic Co-Development, Business Strategies, Industry Trends, and Pipeline Analysis) - Market Size, Trends, and Forecasts A 6Neurotrope Board of Directors Appoints Chairmen Ramat and Freiman as Interim Co-CEOs 2Neurotrope Board of Directors Appoints Chairmen Ramat and Freiman as Interim Co-CEOs 3Neurotrope Board of Directors Appoints Chairmen Ramat and Freiman as Interim Co-CEOs 4
... , , , , For purification of endotoxin-free ultrapure , ... Features and benefits , , Less ... DNA , Greater than 90% recovery of plasmid DNA , ... , , , ...
... , , , A crucial step ... To improve the critical steps in the target-labeling process, , ... for use with Affymetrix GeneChip expression arrays. The GeneChip ... of an ongoing collaboration between , QIAGEN and ...
... , , , , , , ... specific and sensitive multiplex PCR without the need for time-consuming , optimization ... , , ... ready-to-use master mix , High specificity ...
Cached Biology Technology:EndoFree Plasmid Kits 2EndoFree Plasmid Kits 3EndoFree Plasmid Kits 4GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro , transcribed cRNA 2QIAGEN Multiplex PCR Kit 2QIAGEN Multiplex PCR Kit 3
(Date:7/22/2014)... , July 22, 2014 NXT-ID, Inc., ... growing m-commerce market, announces its next generation smart wallet, ... consumer oriented television show that airs on the ... NewsWatch regularly features top technology products ... 31st on the History Channel (6:00-6:30am ET/PT) and ...
(Date:7/21/2014)... professor of entomology and cooperating faculty member ... University of California, Riverside, has been awarded the Silver ... Chemical Ecology ( ISCE ). , Awarded first in ... society,s highest honor. It is awarded annually to scientists ... study of chemicals that mediate interactions between living organisms. ...
(Date:7/21/2014)... Want to conserve water and save on your utility ... and published in the current issue of the journal ... List: The Most Effective Actions U.S. Households Can Take ... water use substantially by simple actions such as installing ... consumption. While the suggestions may be familiar, the researchers ...
Breaking Biology News(10 mins):NXT-ID's Next Generation Wocket Smart Wallet to be Featured on NewsWatch, Airing on History Channel, FYI Network and ION Network 2NXT-ID's Next Generation Wocket Smart Wallet to be Featured on NewsWatch, Airing on History Channel, FYI Network and ION Network 3UC Riverside entomologist receives international honor for chemical ecology contributions 2Researchers provide guide to household water conservation 2Researchers provide guide to household water conservation 3
... Texas patient has been enrolled by researchers at The University ... country,s first double-blind clinical trial studying the safety and efficacy ... up to 19 days after an ischemic stroke. The ... a regenerative therapy developed by Aldagen that uses a patient,s ...
... unique properties, which can be used in future drug ... Release confirms that camel blood can help scientists ... the camelid family have particular heavy-chain antibodies. These antibodies ... therapeutic proteins. One of the most powerful advantages of ...
... study determined that women with generalized tonic-clonic seizures (GTCS) ... cycles where an egg is not releasedthan in cycles ... in Epilepsia , a journal of the International ... role in GTCS occurrence. Medical evidence has ...
Cached Biology News:Study uses new stem cell therapy in patients up to 19 days after stroke 2Cancer gene therapy from camels 2Greater seizure frequency seen in women with epilepsy during anovulatory cycle 2
...
Mol wt: monoisotopic mol wt1,045.5423 Da by calculation...
... for cell culture scale-up, these culture chambers ... - 10 9 range and are ... spinner flasks. • High mechanical • strength ... prior to shipping • Certified nonpyrogenic and ...
Anti 4-hydroxy-2-hexenal (4-HHE) Monoclonal Antibody (HNEJ-2)...
Biology Products: