Navigation Links
AMRESCO’s New Fluorescent DNA Quantitation Kit Uses a Highly Specific DNA-binding Dye that Allows Accurate Quantitation Even in Crude Extracts
Date:5/6/2013

Solon, Ohio (PRWEB) May 06, 2013

DNA quantitation is crucial to experimental design and interpretation of results, especially in sensitive molecular biology applications such as PCR, or for any comparative analysis of multi-source DNA samples. Although UV spectrophotometric measurement at 260 nm is the most widely practiced method of DNA quantitation, it is limited by high background interference from common impurities, such as RNA and protein. Better accuracy and sensitivity can be achieved using AMRESCO’s new Fluorescent DNA Quantitation Kit. The kit utilizes the fluorescent DNA intercalator, bisBenzimide H33258, otherwise known as Hoechst dye, and a simple method that may be easily scaled for a single cuvette or for high throughput in multi-well plates.

AMRESCO's Fluorescent DNA Quantitation kit includes Fluorescent DNA Dye Solution, Fluorescent DNA Standard and Fluorescent DNA Assay Buffer, 10X in volumes sufficient to perform up to 750 reactions. Assays are performed by fluorometric reading of a DNA standard series and unknown samples prepared in diluted Fluorescent DNA Dye Solution. The selective binding of the bisBenizimide H33258 dye to A-T base pairs results in significant fluorescent emission at 460 nm in the presence of DNA. The dye has low affinity for RNA, which improves specificity and sensitivity of the assay results when using impure samples. The assay also tolerates protein contamination that may be present in crude extracts and various buffer compositions commonly used in DNA extraction. The sensitivity of DNA detection with AMRESCO's Fluorescent DNA Quantitation Kit ranges from the low nanogram range (10 ng/mL) up to 5 micrograms per mL.

For more information about the Fluorescent DNA Quantitation Kit or any other AMRESCO products, visit http://www.amresco-inc.com or call 1-800-448-4442.

AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for molecular biology, life sciences, proteomics, histology and clinical areas of research. The company is ISO certified to ensure customers receive the highest quality products.

Read the full story at http://www.prweb.com/releases/2013/5/prweb10697540.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. AMRESCO’s New VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits Offer Long-lasting, High Sensitivity Detection
2. ATCC Announces Agreement with Life Technologies Corporation to Sell and Distribute Green Fluorescent Protein (GFP)-based Materials
3. Goodbye, fluorescent light bulbs! See your office in a new light
4. Research on China's FISH Probe (Fluorescent in Situ Hybridization Probe) Industry, 2013-2017
5. UCLA team develops highly efficient method for creating flexible, transparent electrodes
6. Stanford engineers perfecting carbon nanotubes for highly energy-efficient computing
7. Researchers create highly conductive and elastic conductors using silver nanowires
8. Highly efficient production of advanced biofuel by metabolically engineered microorganism
9. Highly lethal Ebola virus has diagnostic Achilles heel for biothreat detection, scientists say
10. Medrio, a Leading EDC and eClinical SaaS Provider for Clinical Research, Announces the Highly Anticipated Release of Medrio 8.1 with iPad Support
11. New Resource Provides Herb Industry With Sources of Quality Specifications
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/23/2017)... , Feb. 23, 2017 Aviva ... tools, announced the acquisition of GenWay Biotech Incorporated, ... comprehensive service and product offering for both the ... will facilitate growth and enhance capabilities for both ... and ELISA assays will nicely complement ASB,s objective ...
(Date:2/23/2017)... UK (PRWEB) , ... February 22, 2017 , ... ... optics and photonics , have been named Fellows of the Society this year, ... technical contributions in the multidisciplinary fields of optics, photonics, and imaging as well ...
(Date:2/22/2017)... ... February 22, 2017 , ... ... has acquired Kendall Research Systems, LLC (KRS) clinical development program. KRS ... neural interface technology for research and clinical applications. The terms of the transaction ...
(Date:2/22/2017)... 22, 2017  PrimeVax Immuno-Oncology, Inc. announced today its ... the Annual Biocom Global Life Science Partnering Conference.  The ... AM, at the Torrey Pines Lodge, in San Diego.  ... at Biocom who have chosen our company, amongst numerous ... companies, investors, and clinical researchers," said Mr. Chen. "In ...
Breaking Biology Technology:
(Date:2/21/2017)... LONDON , February 21, 2017 ... um 70 Millionen US-Dollar wachsen. Nach einem Gespräch mit mehr ... es einige Hindernisse zu überwinden gilt, um diese Prognose ... ... unter anderem die Mobilisierung der finanziellen Mittel für die ...
(Date:2/13/2017)... , Feb. 13, 2017  RSA Conference -- ... platform that is designed to enhance fraud detection ... release in the RSA Fraud & Risk Intelligence ... organizations to leverage additional insights from internal and ... to better protect their customers from targeted cybercrime ...
(Date:2/9/2017)... , Feb. 9, 2017 The biomass boiler ... of the biomass boiler market globally in terms of ... biomass boilers. The market for biomass boilers has been ... end-user, application, and country/region. The market based on feedstock ... forest residues, biogas & energy crops, urban residues, and ...
Breaking Biology News(10 mins):