Navigation Links
AMRESCO’s New Fluorescent DNA Quantitation Kit Uses a Highly Specific DNA-binding Dye that Allows Accurate Quantitation Even in Crude Extracts
Date:5/6/2013

Solon, Ohio (PRWEB) May 06, 2013

DNA quantitation is crucial to experimental design and interpretation of results, especially in sensitive molecular biology applications such as PCR, or for any comparative analysis of multi-source DNA samples. Although UV spectrophotometric measurement at 260 nm is the most widely practiced method of DNA quantitation, it is limited by high background interference from common impurities, such as RNA and protein. Better accuracy and sensitivity can be achieved using AMRESCO’s new Fluorescent DNA Quantitation Kit. The kit utilizes the fluorescent DNA intercalator, bisBenzimide H33258, otherwise known as Hoechst dye, and a simple method that may be easily scaled for a single cuvette or for high throughput in multi-well plates.

AMRESCO's Fluorescent DNA Quantitation kit includes Fluorescent DNA Dye Solution, Fluorescent DNA Standard and Fluorescent DNA Assay Buffer, 10X in volumes sufficient to perform up to 750 reactions. Assays are performed by fluorometric reading of a DNA standard series and unknown samples prepared in diluted Fluorescent DNA Dye Solution. The selective binding of the bisBenizimide H33258 dye to A-T base pairs results in significant fluorescent emission at 460 nm in the presence of DNA. The dye has low affinity for RNA, which improves specificity and sensitivity of the assay results when using impure samples. The assay also tolerates protein contamination that may be present in crude extracts and various buffer compositions commonly used in DNA extraction. The sensitivity of DNA detection with AMRESCO's Fluorescent DNA Quantitation Kit ranges from the low nanogram range (10 ng/mL) up to 5 micrograms per mL.

For more information about the Fluorescent DNA Quantitation Kit or any other AMRESCO products, visit http://www.amresco-inc.com or call 1-800-448-4442.

AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for molecular biology, life sciences, proteomics, histology and clinical areas of research. The company is ISO certified to ensure customers receive the highest quality products.

Read the full story at http://www.prweb.com/releases/2013/5/prweb10697540.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. AMRESCO’s New VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits Offer Long-lasting, High Sensitivity Detection
2. ATCC Announces Agreement with Life Technologies Corporation to Sell and Distribute Green Fluorescent Protein (GFP)-based Materials
3. Goodbye, fluorescent light bulbs! See your office in a new light
4. Research on China's FISH Probe (Fluorescent in Situ Hybridization Probe) Industry, 2013-2017
5. UCLA team develops highly efficient method for creating flexible, transparent electrodes
6. Stanford engineers perfecting carbon nanotubes for highly energy-efficient computing
7. Researchers create highly conductive and elastic conductors using silver nanowires
8. Highly efficient production of advanced biofuel by metabolically engineered microorganism
9. Highly lethal Ebola virus has diagnostic Achilles heel for biothreat detection, scientists say
10. Medrio, a Leading EDC and eClinical SaaS Provider for Clinical Research, Announces the Highly Anticipated Release of Medrio 8.1 with iPad Support
11. New Resource Provides Herb Industry With Sources of Quality Specifications
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/21/2017)... ... August 21, 2017 , ... Today Aether announced that ... funded by a Bill and Melinda Gates Foundation grant, to pursue a ... Melinda Gates Foundation, also known as the Gates Foundation, is said to be the ...
(Date:8/17/2017)... Westlake Village, CA (PRWEB) , ... August 17, ... ... biopsy technology for cancer research and personalized medicine, today announced the launch of ... in Kansas City, Missouri. The study’s goal is to evaluate the potential for ...
(Date:8/16/2017)... ... 2017 , ... While art and science are often thought of as two ... think. A Mesh Is Also a Snare, a group exhibition presented by the ... Klein Gallery (EKG) on August 17 and run through September 30. An opening reception ...
(Date:8/16/2017)... England , Aug. 16, 2017  Kingfisher Talent, the ... and leadership development, and Virdis Group, global executive search specialists ... alliance that enables clients to leverage the expertise and reach ... our clients here in the Boston ... population of leadership talent throughout the US, Canada ...
Breaking Biology Technology:
(Date:4/11/2017)... GARDENS, Fla. , April 11, 2017 /PRNewswire/ ... management and secure authentication solutions, today announced that ... by Intelligence Advanced Research Projects Activity (IARPA) to ... IARPA,s Thor program. "Innovation has been ... and IARPA,s Thor program will allow us to ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
(Date:4/6/2017)... 2017 Forecasts by Product Type ... by End-Use (Transportation & Logistics, Government & Public Sector, ... Fossil Generation Facility, Nuclear Power), Industrial, Retail, Business Organisation ... Are you looking for a definitive report on the ... ...
Breaking Biology News(10 mins):