Navigation Links
AMRESCO’s New Fluorescent DNA Quantitation Kit Uses a Highly Specific DNA-binding Dye that Allows Accurate Quantitation Even in Crude Extracts
Date:5/6/2013

Solon, Ohio (PRWEB) May 06, 2013

DNA quantitation is crucial to experimental design and interpretation of results, especially in sensitive molecular biology applications such as PCR, or for any comparative analysis of multi-source DNA samples. Although UV spectrophotometric measurement at 260 nm is the most widely practiced method of DNA quantitation, it is limited by high background interference from common impurities, such as RNA and protein. Better accuracy and sensitivity can be achieved using AMRESCO’s new Fluorescent DNA Quantitation Kit. The kit utilizes the fluorescent DNA intercalator, bisBenzimide H33258, otherwise known as Hoechst dye, and a simple method that may be easily scaled for a single cuvette or for high throughput in multi-well plates.

AMRESCO's Fluorescent DNA Quantitation kit includes Fluorescent DNA Dye Solution, Fluorescent DNA Standard and Fluorescent DNA Assay Buffer, 10X in volumes sufficient to perform up to 750 reactions. Assays are performed by fluorometric reading of a DNA standard series and unknown samples prepared in diluted Fluorescent DNA Dye Solution. The selective binding of the bisBenizimide H33258 dye to A-T base pairs results in significant fluorescent emission at 460 nm in the presence of DNA. The dye has low affinity for RNA, which improves specificity and sensitivity of the assay results when using impure samples. The assay also tolerates protein contamination that may be present in crude extracts and various buffer compositions commonly used in DNA extraction. The sensitivity of DNA detection with AMRESCO's Fluorescent DNA Quantitation Kit ranges from the low nanogram range (10 ng/mL) up to 5 micrograms per mL.

For more information about the Fluorescent DNA Quantitation Kit or any other AMRESCO products, visit http://www.amresco-inc.com or call 1-800-448-4442.

AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for molecular biology, life sciences, proteomics, histology and clinical areas of research. The company is ISO certified to ensure customers receive the highest quality products.

Read the full story at http://www.prweb.com/releases/2013/5/prweb10697540.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. AMRESCO’s New VisiGlo™ Prime and VisiGlo™ Select HRP Chemiluminescent Substrate Kits Offer Long-lasting, High Sensitivity Detection
2. ATCC Announces Agreement with Life Technologies Corporation to Sell and Distribute Green Fluorescent Protein (GFP)-based Materials
3. Goodbye, fluorescent light bulbs! See your office in a new light
4. Research on China's FISH Probe (Fluorescent in Situ Hybridization Probe) Industry, 2013-2017
5. UCLA team develops highly efficient method for creating flexible, transparent electrodes
6. Stanford engineers perfecting carbon nanotubes for highly energy-efficient computing
7. Researchers create highly conductive and elastic conductors using silver nanowires
8. Highly efficient production of advanced biofuel by metabolically engineered microorganism
9. Highly lethal Ebola virus has diagnostic Achilles heel for biothreat detection, scientists say
10. Medrio, a Leading EDC and eClinical SaaS Provider for Clinical Research, Announces the Highly Anticipated Release of Medrio 8.1 with iPad Support
11. New Resource Provides Herb Industry With Sources of Quality Specifications
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/27/2016)... -- At present, the Biotech sphere is in ... that volatility is what makes this industry interesting to consider. ... Corp. (NASDAQ: SNTA ), CTI BioPharma Corp. (NASDAQ: ... ), and Heat Biologics Inc. (NASDAQ: HTBX ). ... for these stocks at: http://www.activewallst.com/register/ ...
(Date:5/26/2016)... ... 2016 , ... After several promising treatments in Panama using ... of Knowledge in Panama, a 6 year-old Duchenne’s muscular dystrophy patient received his ... following FDA approval of a second application for a single patient, investigational new ...
(Date:5/25/2016)... ... May 25, 2016 , ... The Ankle Plating ... options designed to address fractures of the distal tibia and fibula. This system ... Ankle Plating System 3 is composed of seven plate families that span the ...
(Date:5/25/2016)... ... ... Lady had been battling arthritis since the age of two and at the ... Hannah sought the help of Dr Jeff Christiansen of Superior Veterinary Surgical Solutions ... help with the pain of Lady’s arthritis. Dr Christiansen suggested that in conjunction with ...
Breaking Biology Technology:
(Date:3/29/2016)... March 29, 2016 LegacyXChange, Inc. ... "LEGX" and SelectaDNA/CSI Protect are pleased to announce our ... in a variety of writing instruments, ensuring athletes signatures ... created collectibles from athletes on LegacyXChange will be assured ... the DNA. Bill Bollander , CEO ...
(Date:3/22/2016)... 2016 According to ... for Consumer Industry by Type (Image, Motion, Pressure, ... & IT, Entertainment, Home Appliances, & Wearable ... 2022", published by MarketsandMarkets, the market for ... USD 26.76 Billion by 2022, at a ...
(Date:3/21/2016)... Massachusetts , March 22, 2016 ... facial recognition with passcodes for superior security   ... ), a leading provider of secure digital communications services, ... their biometric technology and offer enterprise customers, particularly those ... secure facial recognition and voice authentication within a mobile ...
Breaking Biology News(10 mins):