Navigation Links
A knockout resource for mouse genetics
Date:6/15/2011

r mouse mutagenesis - to provide resources efficiently and consistently and to release them freely. Previously attempted strategies to develop mouse models on a large scale suffered the twin disadvantages of not producing precise genetic changes and favouring only the genes that were active during the experiment, leaving the remainder unaltered.

The present work solves these problems. The team exploited a system called homologous recombination within mouse embryonic stem cells, which can deliver very precise alteration of any gene in the genome. It is founded on choosing the correct recombinant DNA molecules (vectors) to target genes efficiently.

However, some genes are essential to life of the cell or organism: disruption of these might cause the cell to die and so the mutation would be 'lost' from the project. Crucially, to ensure that all genes can be disrupted, the team developed DNA vectors that create a mutation only when required: gene targeted by the mutation can be identified, but the mutation activated only when it is to be studied.

But in the essential step to realize its ambitions of a comprehensive, freely available resource, the team designed and delivered a 'pipeline' that systematically designs and constructs the vectors, and efficiently introduces the engineered DNA molecules into the mouse embryonic stem cell line developed specifically for these projects.

Finally, by employing a modular approach to the vector design, a number of other valuable resources are created en route to the generation of targeted ES cells: the paper reports that the consortium had produced vectors for more than half of the genes in the mouse genome. All of these outputs are being made available to the mouse research community through the consortium's web portal at http://www.knockoutmouse.org/

"We are producing mutations in embryonic stem cells with greater efficiency and speed than w
'/>"/>

Contact: Don Powell
press.office@sanger.ac.uk
44-122-349-6928
Wellcome Trust Sanger Institute
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. Crescendo Biologics Announces First Triple Knockout Mouse
2. Sigma® Life Science Launches Pre-designed CompoZr® Knockout Zinc Finger Nucleases for Entire Human Genome
3. Sigma® Life Science Launches Suite of Knockout Rats to Model Parkinsons Disease
4. Physicians Expect to Shift More Time to Online Professional Resources in the Coming Years
5. The Michigan Life Sciences Pipeline Adds More Resources On Its Website
6. Ikaria(R) Strengthens Commercial Operations and Human Resources Leadership
7. BioSpace Launches Life Science Career Content and Resources from the Biotech Work Portal
8. Simcere Pharmaceutical Group Announces Two Senior Promotions and Appointment of New Vice President of Human Resources
9. New Safe Lifting Patient Safety Resource for Nursing School Educators and Students
10. The Michigan Life Sciences Pipeline Announces The Funding Place(TM) and Other Resources to Connect Investors and Startups
11. PharmSource Introduces Proprietary Resource for Identifying New Business Opportunities in Bio/Pharma
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/27/2015)... GlassesOff (OTCBB: GLSO), a visual neuroscience ... human vision system, announced today a partnership with eight-time All-Star, ... Paul to develop a new mobile app designed for ... real-life on-court performance. Vision is the inception of ... a ball or blocking a pass. The critical impact vision ...
(Date:8/26/2015)... ... August 26, 2015 , ... MediVet Biologics, ... second US laboratory is to open in Manhattan, Kansas in early October, 2015. ... long-term growth of research and development through collaboration with researchers from Kansas State ...
(Date:8/26/2015)... 26, 2015  Platform therapeutic company Symic Biomedical, ... a $1.5M Phase II SBIR grant from the ... its therapeutic agent to reduce arteriovenous fistula (AVF) ... stage renal disease (ESRD) patients undergoing hemodialysis. The ... Diabetes and Digestive and Kidney Diseases (NIDDK), will ...
(Date:8/26/2015)... Florida , August 26, 2015 ... the biggest factors fueling the optimism in biotech,s ... M&A,s, collaborations and life sciences advancements.  Active biotechnology ... are Pressure BioSciences, Inc. (OTCQB: PBIO), Sequenom, Inc. ... SRPT ), Raptor Pharmaceutical Corp. (NASDAQ: ...
Breaking Biology Technology:Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 2Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 3New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 2New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 3New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 4New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 5New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 6New Collaborations & Mergers For Research of Molecular Biomarkers in Human Diseases & Clinical Trials for Cancer Therapies Fueling Growth in Multi-Billion Dollar Life Sciences Market 7
... to the community and its biotech ... ... LLY ) announced today completion of the final phase of its $1 ... a ceremony at its Indianapolis operations, Lilly officials, along,with participation by Indianapolis ...
... Market Open on Monday, ... May 19th ... a clinical stage pharmaceutical company committed to,discovering, developing and commercializing novel drugs ... to join the,NASDAQ Biotechnology Index effective with the market open on May ...
... will highlight choice of different modalities ... on single integrated imaging platform., SAN DIEGO, and ... ), a leading manufacturer and developer of,intravascular ultrasound (IVUS), ... to enhance the diagnosis and,treatment of coronary and peripheral ...
Cached Biology Technology:Lilly Completes Final Phase of Its Indianapolis Biotechnology Complex 2Lilly Completes Final Phase of Its Indianapolis Biotechnology Complex 3Pharmasset Selected to Join the NASDAQ Biotechnology Index 2Pharmasset Selected to Join the NASDAQ Biotechnology Index 3Volcano Announces Placement of 1,000th s5 and s5i IVUS Imaging System 2Volcano Announces Placement of 1,000th s5 and s5i IVUS Imaging System 3Volcano Announces Placement of 1,000th s5 and s5i IVUS Imaging System 4
(Date:8/20/2015)... Calif. , Aug. 20, 2015 ... on consumers that are active and healthy. However, ... in the hospital environment to help improve diagnostic ... a video from the Wearable Technologies Conference ... discussed new applications for wearables in healthcare.    ...
(Date:8/18/2015)... 18, 2015  Navitas today announced that ... the Submission Content Management webinar scheduled on ... forum. Dedicated to the Implementation of Regulatory Submission ... for industry, vendors, health authorities, consultants and ... towards a standard method of implementing electronic ...
(Date:8/12/2015)... SPRINGS, Florida , August 12, 2015 ... the pace of mobile payment innovation and advanced biometrics ... to replace the way consumers rely on using their ... ahead with reinventing the future for payment services led ... (NASDAQ: NXTD ), :  Google, Inc. (NASDAQ: ...
Breaking Biology News(10 mins):Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3Navitas, a Key Contributor in IRISS Forum Webinar 2Navitas, a Key Contributor in IRISS Forum Webinar 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 4Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 6
... is one of the hottest ideas in science but has ... Scientists with the U.S. Department of Energy,s Lawrence Berkeley National ... pearl to create what may well be the toughest ceramic ... water of an aluminum oxide (alumina) and the addition of ...
... the Babraham Institute near Cambridge, supported by the ... Sciences Research Council (BBSRC), have discovered that the ... diseases of ageing such as Alzheimer,s disease. The ... Brain . Alzheimer,s disease causes nerve cells ...
... world,s leading publisher of scientific information, announced today the ... Award. Prof. J J Theo Groothuizen, S&T Counsellor, ... at Le Meridien in New Delhi. The award ... researchers in India who have made significant contributions to ...
Cached Biology News:Scientists create tough ceramic that mimics mother of pearl 2Scientists create tough ceramic that mimics mother of pearl 3Maintaining the brain's wiring in aging and disease 2Elsevier announces winners of 3rd Annual Scopus Young Indian Scientist Award 2
Mouse Anti-Human Killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4 (KIR2DL4 Monoclonal Antibody Family: New...
Mouse monoclonal [7B9] to beta II Tubulin ( Abpromise for all tested applications). Antigen: Synthetic peptide, CEEEEGEDEA found at the c-terminus of beta II tubulin. Entrez GeneID: 10383 ...
Gridded Cell Culture Petri Dish (20 mm molded in base), tissue-culture treated...
...
Biology Products: