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Metabolic engineer synthesizes key breast milk ingredient, makes research possible
Date:9/10/2012

URBANA - A University of Illinois microbial engineer has synthesized a sugar in human milk that is thought to protect babies from pathogens. That's important because 2FL, the shorthand scientists use to describe this human milk oligosaccharide (HMO), has not been added to infant formula because HMOs are incredibly expensive.

"We know these oligosaccharides play a vital role in developing a breast-fed baby's gut microbiota and in strengthening their immunity. 2FL (2-fucosyllactose) is the most abundant HMO in breast milk," said Michael Miller, a U of I professor of food microbiology.

To learn more about the HMO's function, Miller would like to do research with 2FL in newborn piglets, an excellent model for the human infant.

Unfortunately, 1 milligram of 2FL costs $100, meaning a single study would require $1 million for the HMO alone, he said.

Microbiologist, meet metabolic engineer. Yong-Su Jin, a departmental colleague and professor in the U of I's Institute for Genomic Biology, believed he could synthesize this oligosaccharide found in breast milk using a strain of E. coli engineered for that purpose.

A new postdoctoral researcher in Jin's laboratory had done some of the legwork for such a project in Korea, and they used their combined experience and expertise to engineer an HMO that can be produced very cheaply and quickly: 1 gram of 2FL per liter of E. coli broth. That means it's possible to produce 2FL in the lab, making Miller's piglet research feasible, he said.

"E. coli makes a starting material for 2FL as part of its normal metabolism, and that suggested to us that it was possible to use E. coli to produce 2FL," Jin said.

"The trick is to get the E. coli cells to increase their production of the starting material (GDP-fucose), which we did by overexpressing the pre-existing biosynthetic pathway. Then we had to give it the ability to transfer GDP-fucose
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Contact: Phyllis Picklesimer
p-pickle@illinois.edu
217-244-2827
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

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