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pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies

ProductspWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies
Company EPICENTRE Biotechnologies
Item pWEB::TNC Deletion Cosmid Transposition Kit
Price $350.00
Description The pWEB::TNC™ Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy and highly reproducible kit protocols. First, genomic DNA is randomly sheared by passing it through a syringe needle. Next, the sheared DNA is end-repaired using reagents in the kit, and then size selected on a gel by comparison with a supplied 40-kb standard. Finally, the size-selected DNA is ligated into the supplied linearized and dephosphorylated pWEB::TNC Cosmid Vector, packaged using ultra-high efficiency MaxPlax™ Lambda Packaging Extracts (>109 pfu/mg for phage lambda), also in the kit, and plated on the supplied EPBOS™ plating cells.
Info EPICENTRE BiotechnologiesEPICENTRE Biotechnologies
726 Post Road
Madison, WI 53713
USA
Customer Service: 800-284-8474
Tech Support: 800-284-8474
Fax Number: 608-442-6114
Web Site: http://www.epibio.com
NOTE: Price information is approximate list price and actual prices may vary.

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Prepared by multi-temperature ethanol fractionation with extensive charcoal treatment and dialysis.• Assay: ≥ 95 % (HPCE)• pH ~5.2 (10 mg/mL in 0.15 M NaCl)...
Collected from animals that are 16 to 18 months old. Sterile, Cell culture tested, ID clarifier: USA Origin...
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