Navigation Links
pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation

ProductspMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation
Company Mirus Bio Corporation
Item pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector
Price $147.00
Description The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemically or enzymatically, and then transfected into the target cells in vitro or in vivo. A convenient alternative to introduce siRNAs into mammalian cells is transient or stable transfection of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters. An expression cassette is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette can be in a linear fragment of DNA, in a plasmid [2,3,5-8], in a viral vector, or integrated into the genome. The expression cassettes that have been used for generating siRNA in mammalian cells have utilized the human U6, human H1, or mouse U6 RNA polymerase III promoters. These promoters drive expression of short RNA transcripts that can become siRNA.

An siRNA molecule is formed from short complementary sense and anti-sense strands of RNA. The sense strand of the siRNA has the same sequence as a short region of the mRNA targeted for RNAi. Each siRNA strand may originate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure. The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) or on separate fragments of DNA (sense and anti-sense strand expression cassettes).

Although PCR based siRNA expression cassettes have the advantages of being fast to produce and test, some users may choose to transfect the siRNA expression cassette in the form of a plasmid or to have a large supply of expression cassette DNA readily available. Any of the PCR products generated in the previous sections can be cloned into the siXpress(TM) Template if the target-specific downstream primer used to generate the PCR products include an Xho I or BamH I restriction site. For additional instruction on molecular biology procedures, refer to Sambrook et al.

Info Mirus Bio CorporationMirus Bio Corporation
505 S. Rosa Rd.
Madison, WI 53719

Call Mirus Bio Corporation to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-438-6816
Customer Service: (888) 530-0801 or (608) 441-2852
Fax Number: (608) 441-2849
Web Site: http://www.mirusbio.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
... OrientExpress™ System Modules ... synthesis and directional cloning ... T7 expression vectors ... configuring OrientExpress™ Cloning Systems, ...
...
... While antigen and antibody-based immunoaffinity ... been reused many times without compromising ... extremes and chaotropic agents has precluded ... Western blotting. The CHEMICON Re-Blot ...
Applications: Western blotting ...
Biology Products:
(Date:4/13/2017)... 2017 According to a new market research report ... Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region ... expected to grow from USD 14.30 Billion in 2017 to USD 31.75 ... ... MarketsandMarkets Logo ...
(Date:4/6/2017)... LONDON , April 6, 2017 ... Control, RFID, ANPR, Document Readers, by End-Use (Transportation & ... Energy Facility, Oil, Gas & Fossil Generation Facility, Nuclear ... Healthcare, Educational, Other) Are you looking for ... Authentication sector? ...
(Date:4/3/2017)... 3, 2017  Data captured by IsoCode, ... detected a statistically significant association between the ... treatment and objective response of cancer patients ... predict whether cancer patients will respond to ... well as to improve both pre-infusion potency testing ...
Breaking Biology News(10 mins):
(Date:6/20/2017)... ... , ... Do More with OHAUS , With the launch of the new ... weighing industry, to extending its expertise across the entire laboratory to a range of ... allowing for its customers to 'Do More' in the lab. , Efficiency ...
(Date:6/19/2017)... ... June 19, 2017 , ... A colony of healthy honey ... and tissues by delivering pollen and nectar containing nutrients necessary for growth and survival. ... healthy. , Many recent indicators point to a decline in honey bee health. Sick ...
(Date:6/15/2017)... ... June 15, 2017 , ... Cybrexa Therapeutics, a start-up ... of financing in the amount of $6 million. An investment vehicle affiliated with ... round. , The Series B funding will enable Cybrexa to complete the build-out ...
(Date:6/14/2017)... ... June 14, 2017 , ... Slone Partners welcomed a panel ... the executive search firm, “Building Value in Precision Medicine: Can We Overcome the ... Medicine, led an open discussion with expert panelists Troy Cox, CEO of Foundation ...
Breaking Biology Technology: