Navigation Links
pMIR-mU6, Mouse U6Template (Vector) from Mirus Bio Corporation

ProductspMIR-mU6, Mouse U6Template (Vector) from Mirus Bio Corporation
Company Mirus Bio Corporation
Item pMIR-mU6, Mouse U6Template (Vector)
Price $147.00
Description The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemically or enzymatically, and then transfected into the target cells in vitro or in vivo [1-4]. A convenient alternative to introduce siRNAs into mammalian cells is transient or stable transfection of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters [2,3,5-11]. An expression cassette is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette can be in a linear fragment of DNA [12], in a plasmid [2,3,5-8], in a viral vector [9-11], or integrated into the genome [13]. The expression cassettes that have been used for generating siRNA in mammalian cells have utilized the human U6, human H1, or mouse U6 RNA polymerase III promoters. These promoters drive expression of short RNA transcripts that can become siRNA.

An siRNA molecule is formed from short complementary sense and anti-sense strands of RNA. The sense strand of the siRNA has the same sequence as a short region of the mRNA targeted for RNAi. Each siRNA strand may originate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure [2] (Figure 1). The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) or on separate fragments of DNA (sense and anti-sense strand expression cassettes; Figures 1 and 2).

Although PCR based siRNA expression cassettes have the advantages of being fast to produce and test, some users may choose to transfect the siRNA expression cassette in the form of a plasmid or to have a large supply of expression cassette DNA readily available. Any of the PCR products generated in the previous sections can be cloned into the siXpress(TM) Template if the target-specific downstream primer used to generate the PCR products include an Xho I or BamH I restriction site. For additional instruction on molecular biology procedures, refer to Sambrook et al.

Info Mirus Bio CorporationMirus Bio Corporation
505 S. Rosa Rd.
Madison, WI 53719

Call Mirus Bio Corporation to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-438-6816
Customer Service: (888) 530-0801 or (608) 441-2852
Fax Number: (608) 441-2849
Web Site: http://www.mirusbio.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
COMING SOON: Anti-Mouse C5/C5b, Clone BB5.1. Monoclonal Antibody...
Vesicle Associated Membrane Protein (VAMP 1/2)...
synphilin-1 (V-19)...
Rabbit polyclonal to Presenilin 1, prediluted ( Abpromise for all tested applications). entrezGeneID: 5663 SwissProtID: P49768...
Biology Products:
(Date:11/14/2018)... ... November 14, 2018 , ... In recognition of World Diabetes Day, Diopsys is ... advocacy. The company is reaching out to help educate patients and the healthcare community ... of vision testing for people with the disease. , The American Diabetes Association (ADA) ...
(Date:11/13/2018)... Conn. (PRWEB) , ... November 13, 2018 , ... ... modern gum surgery alternatives with less invasive techniques, less discomfort and ... gum disease symptoms like receding, swollen and bleeding gums, and bad breath, this ...
(Date:11/13/2018)... ... November 13, 2018 , ... ... nation’s largest Phase I-IV clinical trial site service companies in North America, ... practices in North Carolina, and a leading provider of innovative, quality, comprehensive ...
Breaking Biology News(10 mins):
(Date:11/13/2018)... ... November 13, 2018 , ... Sharon Kleyne, founder and new ... Pass, Oregon believes in the power of talk radio education Power of Water® ... the Power of Water® on talk radio every week with the “Sharon Kleyne ...
(Date:11/7/2018)... ... November 07, 2018 , ... ... leading a campaign to increase awareness of the adult tissue stem cell counting ... month of November, the company is intensifying this effort with new educational publications ...
(Date:11/5/2018)... ... November 05, 2018 , ... GlycoMark, Inc., the exclusive provider ... its website, http://www.glycomark.com , as part of National Diabetes Month. The ... the website in November from 9 a.m. – 5 p.m. ET weekdays for ...
(Date:10/31/2018)... ... October 30, 2018 , ... Myoscience, Inc. announced today that ... in Memphis, Tennessee. This study enrolled 125 patients and compares the effect ... pain after total knee replacement surgery (TKA). , The Myoscience iovera° system is ...
Breaking Biology Technology: