Navigation Links
pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation

ProductspMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
Company Mirus Bio Corporation
Item pMIR-hU6-Luc, Human U6 Luciferase Control Vector
Price $147.00
Description The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemically or enzymatically, and then transfected into the target cells in vitro or in vivo. A convenient alternative to introduce siRNAs into mammalian cells is transient or stable transfection of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters. An expression cassette is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette can be in a linear fragment of DNA, in a plasmid [2,3,5-8], in a viral vector, or integrated into the genome. The expression cassettes that have been used for generating siRNA in mammalian cells have utilized the human U6, human H1, or mouse U6 RNA polymerase III promoters. These promoters drive expression of short RNA transcripts that can become siRNA.

An siRNA molecule is formed from short complementary sense and anti-sense strands of RNA. The sense strand of the siRNA has the same sequence as a short region of the mRNA targeted for RNAi. Each siRNA strand may originate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure. The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) or on separate fragments of DNA (sense and anti-sense strand expression cassettes).

PCR Expression Cassettes can be cloned using either the Hind III or EcoR I site upstream of the promoter and Xho I or BamH I site downstream of the siRNA and termination sequence. The cloning sites used must be unique in the PCR product. Hind III and Xho I are recommended because these restriction enzymes function with compatible buffers.

Info Mirus Bio CorporationMirus Bio Corporation
505 S. Rosa Rd.
Madison, WI 53719

Call Mirus Bio Corporation to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-438-6816
Customer Service: (888) 530-0801 or (608) 441-2852
Fax Number: (608) 441-2849
Web Site: http://www.mirusbio.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
5. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
6. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
7. Mouse Anti-Human Procollagen type I C-terminal propeptide (PICP) Monoclonal Antibody, Unconjugated from AntibodyShop A/S
8. Mouse Anti-Human Acetylcholinesterase, brain (AChE) Monoclonal Antibody, Unconjugated, Clone 12B4 from AntibodyShop A/S
9. Mouse Anti-Human a1-Antitrypsin (alpha-1 AT) Monoclonal Antibody, Unconjugated, Clone 4E5 from AntibodyShop A/S
10. Human and Mouse RAR Neutralizing Peptide from ABR-Affinity BioReagents
11. Rabbit Anti-Human Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7 (SIRT7) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
... efficient enrichment of functional endoplasmic reticulum ... culture. The isolated ER are useful ... pathway analysis. Metabolic abnornalities of ER ... cystic fibrosis and many more ER ...
Blasticidin resistance gene (bsr), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
... for stabilizing 400g of purified RNA samples., ... the stabilization of aqueous RNA samples that ... as guanidine, phenol/chloroform, silica, oligo dT etc ... contain all the necessary components for the ...
Hygromycin resistance gene (hph), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
Biology Products:
(Date:6/19/2019)... ... , ... Dahryn Trivedi, today released research results on the beneficial impact of ... to be beneficial for treating multiple bacterial diseases. , The preclinical trial shows:, ... 68% decrease in weight , Over 8% alteration in melting point ...
(Date:6/16/2019)... ... 14, 2019 , ... Emtec, Inc.®, a global IT and ... with Versiti’s CIO at the Healthcare Industry User Group (HIUG) Interact 2019 ... Versiti Vice President and Chief Information Officer, Lynne Briggs, will present Versiti’s Oracle ...
(Date:6/13/2019)... ... 2019 , ... Global Clinical and Ancillary Supply Chain leader ... ancillary supply chain management needs of large- and middle-market pharmaceutical, biotechnology, and medical ... will sponsor a summer education program offered by the Young Men and Women ...
Breaking Biology News(10 mins):
(Date:5/31/2019)... ... , ... World Compliance Seminars today announced a live seminar ... MA. This peer recommended interactive workshop is always selected by professionals at global ... with a compendial treatment of Data Integrity fundamentals. After laying the groundwork, the ...
(Date:5/22/2019)... ... 22, 2019 , ... Artemis (formerly Agrilyst), the leading enterprise Cultivation ... round co-led by Astanor Ventures and Talis Capital with participation ... iSelect Fund . The company has raised $11.75m to date and will use the ...
(Date:5/15/2019)... ... May 16, 2019 , ... Emulsifiers are the ... and beverage products rely on these ingredients to create stable quality food that ... This paradigm shift triggers food formulators to seek out alternatives for starches, gums ...
(Date:5/8/2019)... ... ... hands, Atlas is on the large side for a Quarter Horse. He and his ... When loping around a corner in late 2017, Atlas tripped and partially tore his ... able to walk. Fortunately Atlas’ owner, Kelly, sought the opinion of her veterinarian, Dr. ...
Breaking Biology Technology: