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pET GST Fusion System 42 plus Competent Cells from Novagen

ProductspET GST Fusion System 42 plus Competent Cells from Novagen
Company Novagen
Item pET GST Fusion System 42 plus Competent Cells
Price $450.00
Description Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

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... form culture flask is designed for culturing organisms requiring ... has a heavy beaded rim to protect against chipping ... is 38mm and takes a No. 6.5 rubber stopper. ... heat source smaller than the base of the flask. ...
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... This kit is designed for the fast ... vector plasmids at any position (for the ... FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin ... cells. It combines a prokaryotic promoter (gb2) ...
... a magnetic bead-based PCR purification ... transfer, centrifugation or filtration steps. ... large PCR extension products in ... AMPure can easily be performed ...
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