Navigation Links
pET GST Fusion System 42 from Novagen

ProductspET GST Fusion System 42 from Novagen
Company Novagen
Item pET GST Fusion System 42
Price $373.00
Description Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 41 plus Competent Cells from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
3'-O-Methylguanosine 5'-Triphosphate, Sodium, 1 umol. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
Natural Mouse Laminin is purified from the Engelbreth-Holm-Swarm (EHS) sarcoma and is provided in Tris-buffered saline....
EPH electrode, cathode, 1. Category: Electrophoresis Systems & Standards & Reagents, IEF & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
... adult normal paraffin tissue array is ... proteins of interest in extensive panels ... Rat brain, heart, kidney, liver, lung, ... arrayed on positively charged glass slide. ...
Biology Products:
(Date:1/30/2019)... NEW YORK (PRWEB) , ... ... ... a nonprofit organization that identifies, counsels, and supports high-growth, high-impact life science ... investment professionals representing 10 national nonprofit disease foundations and disease-focused venture funds ...
(Date:1/24/2019)... ... January 23, 2019 , ... rHEALTH ... a Notice of Allowance from the United States Patent and Trademark Office (USPTO) ... company, DNA Medicine Institute (DMI). This application covers the addition of a length ...
(Date:1/20/2019)... DIEGO (PRWEB) , ... January 16, 2019 , ... January ... and cooking events company. Not only has the company already hosted several of ... host Fortune 500 companies, Amazon and Janssen Pharmaceuticals, later this month. , Since ...
Breaking Biology News(10 mins):
(Date:2/12/2019)... ... February 12, 2019 , ... ... finalized a deal to extend their use of Medrio, the leading provider of ... The CRO has been a regular Medrio customer for over nine years, primarily ...
(Date:2/2/2019)... ... February 01, 2019 , ... ... announce the appointment of its new CEO, Julien Dert, being effective on February ... international experience in finance and management, in which more than 12 years in ...
(Date:1/30/2019)... ... , ... K&R Negotiations announced today that Mladen Kresic, CEO and founder of ... Six Ways to Shorten the Sales Cycle : Reduce Complexity and Increase Win Rates, ... , Negotiation expert Kresic will discuss the realities of today’s complex selling environment ...
(Date:1/28/2019)... ... January 28, 2019 , ... ... low molecular weight compounds. In this early stage, false positives can be problematic ... time, it is vital that screening techniques are highly sensitive to detect weak ...
Breaking Biology Technology: