Navigation Links
pET GST Fusion System 42 from Novagen

ProductspET GST Fusion System 42 from Novagen
Company Novagen
Item pET GST Fusion System 42
Price $373.00
Description Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 41 plus Competent Cells from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... Anti-phospho-Hck [pTyr 209/ pSer 211 ] affinity ... in Dulbecco's phosphate buffered saline (without Mg 2+ ... 1.0 mg/ml BSA (IgG and protease free) and ... from the region of mouse Hck that contains ...
... provides a familiar interface for data ... warehousing. The transition from paper to ... LABTrack contains an integrated LIMS, ... rules mechanism. These functions allow LABTrack ...
... a positive control for ChIP assays. Efficient ... vivo is a crucial step for the ... is probably the most important parameter. Two ... should be taken into account: first, an ...
Mouse monoclonal [M2II-12] to MRP2 ( Abpromise for all tested applications). entrezGeneID: 1244 SwissProtID: Q92887...
Biology Products:
(Date:8/14/2018)... ... 14, 2018 , ... Cognition Corporation ®, a software ... their NAVIGATE2018 conference. , Steve McRoberts, Group Head of Quality and ... Cognition’s annual user conference. An experienced quality and regulatory professional for over 20 ...
(Date:8/9/2018)... ... August 08, 2018 , ... Plum Alley Investments, an ... and healthcare companies, has invested in a sought-after $23 million Series A round ... Technology and Mammoth Biosciences , The biotech company is bringing to market the ...
(Date:8/7/2018)... ... August 06, 2018 , ... Passersby can’t help but notice ... University Edwardsville’s University Park. SIUE students are working the “Feedstock Diversity Showcase” that ... feedstocks represent conventional biofuels production, advanced biofuels production and cellulosic biofuels production. , ...
Breaking Biology News(10 mins):
(Date:8/9/2018)... ... ... InTouchMD , the leading provider of data services, outreach and marketing ... Biotech Week Boston , September 4th – 7th at Hynes Convention Center in Boston, ... powerful all in one marketing solution. Pulse is a multi-use platform that helps biotech ...
(Date:8/7/2018)... ... August 06, 2018 , ... ... companies, announces the placement of Dr. Paul Harney as President, North America at ... growth strategy and enhancing client satisfaction. He was also named to Prescient’s Board ...
(Date:7/31/2018)... Ill. (PRWEB) , ... July 31, 2018 , ... ... cooking oil for 65 years, announces the launch of their new website. The ... the user experience throughout the site. Mahoney partnered with Proceed Innovative to build ...
(Date:7/25/2018)... ... July 25, 2018 , ... Vium, Inc. ... Digital Vivarium™ and Vium Cloud approach to accelerate preclinical drug discovery and development, ... the company announced the appointment of Jeff Caron to the newly formed role ...
Breaking Biology Technology: