Navigation Links
pET GST Fusion System 41 from Novagen

ProductspET GST Fusion System 41 from Novagen
Company Novagen
Item pET GST Fusion System 41
Price $373.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 plus Competent Cells from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... This CE-based system provides clear advantages ... (viral load, gene expression), mutation analysis, ... easy-to-use system with microplate connectivity is ... and quality control. The P/ACE ...
The imaging screen-K (Kodak), is a 35 x 43 cm storage phosphor screen that is used for the quantitation and imaging of 32P, 33P, 35S, 14C, and 3H....
The filter 695RDF55 Reflect 635 for the Molecular Imager FX is a band pass filter for Cy5 and Alexa Fluor 635 dyes....
... Kit is a 2-color staining method for ... to detect apoptotic cells by flow cytometry. ... required for measuring apoptosis in cells, including ... reagent performance; washing, reaction and rinsing buffers ...
Biology Products:
(Date:1/8/2019)... ... , ... Join industry experts Julie Peacock, Client Services at Comprehend and Heather ... January 17, 2019 at 1pm EST. , This webinar will dive into best ... Featured speakers will also present a case study of a leading pharmaceutical company that ...
(Date:1/7/2019)... Calif. (PRWEB) , ... January 07, 2019 , ... ... Intelligence (AI) and leading supplier of Graph Database technology for Knowledge Graphs solutions, ... as a 2019 ‘Trend-Setting’ Product . DBTA’s data and information management ...
(Date:1/4/2019)... ... 2019 , ... Stay on top of current hot topics through free webinars ... all webinars is free, so be sure to register today to save your place! ... to check out our upcoming webinars, or click below to learn more about each ...
Breaking Biology News(10 mins):
(Date:12/14/2018)... ... December 14, 2018 , ... NOVA OCULUS ... given the green light to immediately begin a clinical patient trial in Edmonton ... devices bureau of Health Canada has granted what is called an Investigational Testing ...
(Date:12/10/2018)... DIEGO (PRWEB) , ... December ... ... a fully integrated Contract Development and Manufacturing Organization (CDMO) with sites in ... conjugation optimization, cGMP manufacturing, and aseptic fill finish solutions for monoclonal antibody ...
(Date:11/29/2018)... ... November 29, 2018 , ... RAGS, today announced the ... Kickstart Seed Fund, with participation from existing investor and board member Jeremy Andrus, CEO ... of Cotopaxi. The additional funding will enable the company to accelerate its growth and ...
(Date:11/20/2018)... ... November 19, 2018 , ... Patients with missing teeth in Sandy Springs, ... from Dr. Dan Myers at THE DENTIST Centers without a referral. Dr. Myers is ... the last 20 years. He now offers skilled dental care in two practice locations ...
Breaking Biology Technology: