Navigation Links
pET GST Fusion System 41 from Novagen

ProductspET GST Fusion System 41 from Novagen
Company Novagen
Item pET GST Fusion System 41
Price $373.00

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 plus Competent Cells from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... syringe pump series combines Harvard Apparatuss best pump ... a wide range of configuration options. A lookup ... of syringes from all the major manufacturers. A ... the pertinent information from across the lab. All ...
ACS Reagent Grade C 19 H 14 O 5 S Formula Weight: 354.38 PRODUCT SPECIFICATIONS Form: Rust/red crystalline powder Clarity of Solution: Passes test Visual Transition Interval: pH 6.8 - Yellow pH 8.2...
... Vector NTI allows you to store and ... data in a secure central relational database. ... creates an invaluable resource for clone tracking ... easily for immediate analysis. LabShare software integrates ...
... Protein A and Protein G bind ... immunoglobulins. Protein A and Protein G conjugates ... purify immunoglobulins (antibodies) and immunoglobulin subtypes from ... and other biological fluids. These reagents are ...
Biology Products:
(Date:5/19/2015)... , May 19, 2015  Technology is rapidly ... internally and in the cloud. Passwords and their management ... of OTP and standards-based specifications such as those developed ... outmoded use of passwords presents for BYOD, COPE, IoT, ... unified biometric identity protocol. In response ...
(Date:5/14/2015)... 2015  Verificient Technologies, Inc., a technology ... remote proctoring, announced a new alliance partnership ... creator of the Canvas Learning Management System ... will benefit from the seamless integration of ... a fully integrated multifactor biometrics behavioral application, ...
(Date:5/10/2015)... GOTHENBURG, Sweden , May 11, 2015 ... touch fingerprint sensors FPC1025 and FPC1155 from the distributor World ... one of FPC,s distributors in Asia . ... the sensors will be used by smartphone manufacturers in ... is included in the communicated revenue guidance of + 1 000 ...
Breaking Biology News(10 mins):HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Verificient and Instructure Announce Alliance Partnership to Provide Online Proctoring for K-12 and Higher Education Institutions 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2
... an international group of colleagues to delay their race ... 7,000-hour slog through a compendium of 50,000 scientific articles ... that might herald the furtive presence of pancreatic cancer. ... and expensive testing that needs to be done before ...
... Ky. (April 6, 2009) The side effects ... being investigated for a variety of diseases are ... a study by a University of Kentucky researcher. ... in the journal Nature, Dr. Jayakrishna Ambati, a ...
... USA (April 6, 2009) Cell Press, an imprint of ... as well as Current Biology and The ... Special Library Association,s (SLA) Top 100 Journals in Biology and ... is compiled by the 680-plus members of the SLA,s Biomedical ...
Cached Biology News:Compendium of pancreatic cancer biomarkers established as strategic approach to early-detection 2Compendium of pancreatic cancer biomarkers established as strategic approach to early-detection 3Ambati study published in PNAS 2Ambati study published in PNAS 3Three prominent Cell Press journals named among the 100 most influential journals in past 100 years 2
(Date:6/3/2015)... and BRUSSELS, Belgium (PRWEB) June 03, 2015 ... beyond, photonics plays an important part in enabling success ... European Commission. Innovation and technology leaders helped bring focus ... thematic sessions during the Photonics21 annual meeting ... a sponsor of the event, held 28 and 29 ...
(Date:6/2/2015)... Research and Markets ( ) has ... by Product , Test, Application & End User - ... The bioburden testing market is expected to reach ... growing at a CAGR of 9.8% from 2014 to ... limit testing market is segmented into consumables and instruments. ...
(Date:6/2/2015)... Research and Markets( ) has announced ... Application & by Products - Global Forecast to 2020" ... genotyping market is expected to reach $17.0 Billion in ... of 22.3% during the forecast period of 2015 to ... the availability of a range of products for wide ...
(Date:6/2/2015)... , June 2, 2015   BioClinica ® , ... provider, today announced that David Peters , an ... Executive Vice President and Chief Financial Officer (CFO) and ... Peters brings more than 25 years of financial ... "David has extensive and deep knowledge in all areas ...
Breaking Biology Technology:Photonics21 Annual Meeting Focuses on Solutions for ‘Societal Challenges’ 2Photonics21 Annual Meeting Focuses on Solutions for ‘Societal Challenges’ 3Bioburden Testing Market 2015 - Global Forecasts to 2019 2Genotyping Market Report 2015 by Technology, Application & by Products - Global Forecast to 2020 for the $17 Billion Market 2BioClinica Names David Peters New Chief Financial Officer 2